Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free

• WB

Lab

Western blot - Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (AB232559)

This data was developed using the same antibody clone in a different buffer formulation (ab126600).

Lanes 1-4 : Merged signal (red and green). Green - ab126600 observed at 80 kDa. Red - loading control ab8245 observed at 36 kDa.

ab126600 Anti-Calpain 2 antibody [EPR5977] was shown to specifically react with Calpain 2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266628 (knockout cell lysate ab257379) was used. Wild-type and Calpain 2 knockout samples were subjected to SDS-PAGE. ab126600 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Calpain 2 antibody [EPR5977] (<a href='/en-us/products/primary-antibodies/calpain-2-antibody-epr5977-ab126600'>ab126600</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

CAPN2 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human CAPN2 (Calpain 2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-capn2-calpain-2-knockout-hek-293t-cell-line-ab266628'>ab266628</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

LNCaP cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (AB232559)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126600).

All lanes:

Western blot - Anti-Calpain 2 antibody [EPR5977] - BSA and Azide free (ab232559) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 80 kDa

false