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AB28280

Anti-Calpain 5 antibody

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(3 Publications)

Rabbit Polyclonal Calpain 5 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN5 aa 1-50.

View Alternative Names

NCL3, CAPN5, Calpain-5, Calpain htra-3, New calpain 3, nCL-3

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Calpain 5 antibody (AB28280)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 5 antibody (AB28280)

ICC/IF image of ab28280 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human CAPN5 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O15484

Specificity

Does not cross react with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.).

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Complementary Products

Primary Antibodies

AB108400

Anti-Calpain 1 antibody [EPR3319]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 1 antibody [EPR3319] (AB108400)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB126600

Anti-Calpain 2 antibody [EPR5977]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Calpain 2 antibody [EPR5977] (AB126600)

  • 0
  • 1
Primary Antibodies

AB76020

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (AB76020)

  • 5
  • 18
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.

ab28280 does not recognize amino-processed Calpain 5, and can be used to discriminate between latent and amino-processed forms. ab28280 will also bind to the non-reduced protein, and thus may have application for immunoprecipitation, immunohistochemistry, and ELISA.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody has been peptide-affinity purified.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 5 also known as PalBH is a non-classical member of the calpain family with a mass of approximately 80 kilodaltons. Calpain 5 is made from a calcium-dependent cysteine protease showing expression in tissues like the retina and neurons. Known for its calpain-like protease domain and an extended C-terminal region Calpain 5 lacks the typical EF-hand motifs found in other calpains.
Biological function summary

Calpain 5 acts without forming complexes with calmodulin or calpastatin which is common in other calpain family members. It modulates cellular processes such as cytoskeleton remodeling and cellular signaling. It plays roles in cell cycle progression and apoptosis by regulating molecules involved in these processes. Calpain 5 interacts with cytoskeletal proteins and promotes degradation or remodeling facilitating normal cellular function and adaptation to stress.

Pathways

Calpain 5 functions are relevant in signal transduction and apoptosis pathways. In signal transduction it participates in protein kinase C-related pathways where it modulates protein activity critical for cell communication. Regarding apoptosis Calpain 5 interacts with BCL-2 family proteins which are key regulators of cell death influencing cell survival outcomes.

Mutations or dysregulation of Calpain 5 associate with retinal disorders like vitelliform macular dystrophy and cone-rod dystrophy. These conditions involve Calpain 5 in the deterioration of retinal cells likely through interactions with retinal proteins such as CRX and RDH12. Understanding Calpain 5's role in these disorders may offer insights into new therapeutic approaches for degeneration of retinal cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-regulated non-lysosomal thiol-protease.
See full target information CAPN5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular brain 12:54 PubMed31164142

2019

Olfactory receptor neurons express olfactory marker protein but not calpain 5 from the same genomic locus.

Applications

Unspecified application

Species

Unspecified reactive species

Noriyuki Nakashima,Kie Nakashima,Akiko Takaku-Nakashima,Makoto Takano

Investigative ophthalmology & visual science 57:2509-21 PubMed27152965

2016

Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Kellie A Schaefer,Marcus A Toral,Gabriel Velez,Allison J Cox,Sheila A Baker,Nicholas C Borcherding,Diana F Colgan,Vimala Bondada,Charles B Mashburn,Chen-Guang Yu,James W Geddes,Stephen H Tsang,Alexander G Bassuk,Vinit B Mahajan

The Journal of biological chemistry 289:19383-94 PubMed24838245

2014

Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies.

Applications

WB, WB

Species

Human, Rat

Ranjana Singh,M Kathryn Brewer,Charles B Mashburn,Dingyuan Lou,Vimala Bondada,Brantley Graham,James W Geddes
View all publications
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Product promise

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