AB317224

Anti-Calpain 5 antibody [HL2432]

Name: Anti-Calpain 5 antibody [HL2432] (ab317224) | Abcam
Brand: Abcam Limited
SKU: AB317224

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Rabbit Monoclonal Calpain 5 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CAPN5.

View Alternative Names

NCL3, CAPN5, Calpain-5, Calpain htra-3, New calpain 3, nCL-3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 5 antibody [HL2432] (AB317224)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 5 antibody [HL2432] (AB317224)

ab317224 detects Calpain 5 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. Calpain 5 stained by Calpain 5 antibody ab317224 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 5 antibody [HL2432] (AB317224)

ab317224 detects secreted Calpain 5 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat stomach. Calpain 5 stained by ab317224 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Supplier Data

Western blot - Anti-Calpain 5 antibody [HL2432] (AB317224)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317224 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Calpain 5 antibody [HL2432] (ab317224) at 1/1000 dilution

Lane 1:

RMS 13 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-Calpain 5 antibody [HL2432] (AB317224)

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317224 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Calpain 5 antibody [HL2432] (ab317224) at 1/1000 dilution

Lane 1:

Mouse uterus tissue extracts at 50 µg

Lane 2:

Mouse stomach tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2432

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CAPN5.

O15484

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 5 also known as PalBH is a non-classical member of the calpain family with a mass of approximately 80 kilodaltons. Calpain 5 is made from a calcium-dependent cysteine protease showing expression in tissues like the retina and neurons. Known for its calpain-like protease domain and an extended C-terminal region Calpain 5 lacks the typical EF-hand motifs found in other calpains.

Biological function summary

Calpain 5 acts without forming complexes with calmodulin or calpastatin which is common in other calpain family members. It modulates cellular processes such as cytoskeleton remodeling and cellular signaling. It plays roles in cell cycle progression and apoptosis by regulating molecules involved in these processes. Calpain 5 interacts with cytoskeletal proteins and promotes degradation or remodeling facilitating normal cellular function and adaptation to stress.

Pathways

Calpain 5 functions are relevant in signal transduction and apoptosis pathways. In signal transduction it participates in protein kinase C-related pathways where it modulates protein activity critical for cell communication. Regarding apoptosis Calpain 5 interacts with BCL-2 family proteins which are key regulators of cell death influencing cell survival outcomes.

Mutations or dysregulation of Calpain 5 associate with retinal disorders like vitelliform macular dystrophy and cone-rod dystrophy. These conditions involve Calpain 5 in the deterioration of retinal cells likely through interactions with retinal proteins such as CRX and RDH12. Understanding Calpain 5's role in these disorders may offer insights into new therapeutic approaches for degeneration of retinal cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-regulated non-lysosomal thiol-protease.

See full target information CAPN5

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Product promise

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