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AB76974

Anti-Calpain 6 antibody

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(3 Publications)

Rabbit Polyclonal Calpain 6 antibody. Suitable for WB and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN6.

View Alternative Names

CALPM, CANPX, CAPN6, Calpain-6, Calpain-like protease X-linked, Calpamodulin, CalpM

1 Images
Western blot - Anti-Calpain 6 antibody (AB76974)
  • WB

Unknown

Western blot - Anti-Calpain 6 antibody (AB76974)

Lane 1:

Western blot - Anti-Calpain 6 antibody (ab76974) at 0.5 µg/mL

Lane 2:

Western blot - Anti-Calpain 6 antibody (ab76974) at 1 µg/mL

All lanes:

rat lung tissue lysate

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CAPN6. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6Q1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Affinity chromatography purified via peptide column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 6 also known as CAPN6 is a protease without proteolytic activity and belongs to the calpain family. This protein weighs around 75 kDa and expresses mainly in the placenta embryonic tissues and a variety of tumor cells. Unlike other calpains CAPN6 does not have a conserved catalytic triad and acts as a scaffold rather than an proteolytic enzyme. It participates in cytoskeletal remodeling due to its strong interactions with microtubules.
Biological function summary

CAPN6 is involved in cell proliferation migration and growth regulation processes. The protein associates with microtubules contributing to the regulation of cellular mechanics and morphology. It interacts with other cellular components to support functions in tissue development and is important in maintaining cytoskeleton dynamics. CAPN6's engagement in cellular processes indicates its potential role as a cellular signaling mediator.

Pathways

CAPN6 is significantly involved in the Wnt signaling and cytoskeletal remodeling pathways. These pathways are essential for cellular growth and differentiation where CAPN6 modulates cytoskeletal rearrangements and influences these biological processes. In the Wnt pathway CAPN6 interacts with proteins like Dishevelled and influences the cellular structure and signaling outcomes involved in the pathway’s regulation.

CAPN6 is associated with various types of cancer and fibrosis processes. The aberrant expression of CAPN6 can lead to dysregulation in cell proliferation and mobility which connects directly to cancer progression especially in embryonic tumors and other malignancies. In cancer contexts CAPN6 associates with proteins such as β-catenin amplifying oncogenic signals through Wnt signaling making it a potential target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues (By similarity).
See full target information CAPN6
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:378 PubMed38939621

2024

Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wu-Fu Chen,Jimmy Ming-Jung Chuang,San-Nan Yang,Nan-Fu Chen,Manojit Bhattacharya,Hsin-Tzu Liu,Kuldeep Dhama,Chiranjib Chakraborty,Zhi-Hong Wen

International journal of molecular medicine 48: PubMed34435644

2021

Calpain 6 inhibits autophagy in inflammatory environments: A preliminary study on myoblasts and a chronic kidney disease rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Yue Zhang,Li Jie Gu,Nan Zhu,Ling Wang,Min Chao Cai,Jie Shuang Jia,Shu Rong,Wei Jie Yuan

Veterinary research 44:14 PubMed23497022

2013

Gene and protein patterns of potential prion-related markers in the central nervous system of clinical and preclinical infected sheep.

Applications

IHC-P

Species

Sheep

Hicham Filali,Enric Vidal,Rosa Bolea,Mercedes Márquez,Paola Marco,Antonia Vargas,Martí Pumarola,Inmaculada Martin-Burriel,Juan J Badiola
View all publications
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Product promise

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