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AB250404

Anti-Calpain 9 antibody [EPR13422] - BSA and Azide free

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Rabbit Recombinant Monoclonal Calpain 9 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

NCL4, CAPN9, Calpain-9, Digestive tract-specific calpain, New calpain 4, Protein CG36, nCL-4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Calpain 9 antibody [EPR13422] - BSA and Azide free (AB250404)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calpain 9 antibody [EPR13422] - BSA and Azide free (AB250404)

This data was developed using ab181178, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling Calpain 9 with ab181178 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot - Anti-Calpain 9 antibody [EPR13422] - BSA and Azide free (AB250404)
  • WB

Supplier Data

Western blot - Anti-Calpain 9 antibody [EPR13422] - BSA and Azide free (AB250404)

This data was developed using ab181178, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calpain 9 antibody [EPR13422] (<a href='/en-us/products/primary-antibodies/calpain-9-antibody-epr13422-ab181178'>ab181178</a>) at 1/10000 dilution

All lanes:

Human stomach lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 79 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13422

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250404 is the carrier-free version of ab181178.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 9 also known as CAPN9 is a calcium-dependent cysteine protease enzyme that plays a role in proteolytic processes. It has a molecular mass of approximately 114 kDa. Capable of autolytic activation Calpain 9 becomes functional in the presence of calcium ions. Expression of Calpain 9 occurs mainly in the gastrointestinal tract particularly in the stomach and small intestine. This enzyme has distinct activities compared to other calpains partially due to its unique conserved domains affecting substrate specificity.
Biological function summary

The enzyme assists in maintaining cellular homeostasis through its proteolytic functions. Calpain 9 does not usually exist as part of large protein complexes but it interacts dynamically with substrates and other cellular components. It participates in various cellular processes such as cell cycling differentiation and apoptosis. Essentially Calpain 9 regulates specific protein modifications and degradations which affect cellular structural integrity and signal transduction mechanisms.

Pathways

Calpain 9 influences intracellular signaling and remodeling being particularly linked to the non-canonical Wnt signaling pathway and calcium signaling pathways. Within these pathways Calpain 9 works alongside other related proteins such as GSK-3β in the Wnt pathway and calmodulin in calcium signaling. These pathways are important for the modulation of cellular responses to stimuli and contribute to the fine-tuning of cellular activities impacting tissue development and repair processes.

The dysregulation of Calpain 9 takes part in gastric cancer progression and susceptibility to inflammatory bowel disease (IBD). It contributes to tumorigenesis when proteolytic balance within cellular environments shifts unfavorably. Furthermore alterations in Calpain 9 expression or activity influence interactions with proteins like E-cadherin within cancer progression. Understanding these relationships may provide insights into novel therapeutic targets or diagnostic markers for specific diseases involving impaired protease regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-regulated non-lysosomal thiol-protease.
See full target information CAPN9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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