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AB28237

Anti-Calpain small subunit 1 antibody

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(8 Publications )

Rabbit Polyclonal Calpain small subunit 1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CAPNS1.

View Alternative Names

CAPN4, CAPNS, CAPNS1, Calpain small subunit 1, CSS1, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calcium-dependent protease small subunit 1, Calpain regulatory subunit, CANP small subunit, CDPS

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody (AB28237)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody (AB28237)

ab28237 staining Human normal renal medulla. Staining is localized the nucleus and cytoplasm.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-Calpain small subunit 1 antibody (AB28237)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calpain small subunit 1 antibody (AB28237)

ICC/IF image of ab28237 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human CAPNS1. The exact immunogen used to generate this antibody is proprietary information.

P04632

Specificity

ab28237 binds to calpain S-1, does not cross react with the other calpain family members (calpain S-2, calpain-1, calpain-2, calpain-3, etc.).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain small subunit 1 also known as CSS1 or CAPNS1 is a small protein with a molecular mass of approximately 28 kDa. CSS1 forms an essential part of the calpain protease system which includes both the catalytic large and regulatory small subunits. CSS1 is important for the stability and function of the calpain holoenzyme. It expresses in various tissues including brain muscle and kidney suggesting its widespread functional role in cellular processes.
Biological function summary

Several cellular processes involve the calpain protease system to which Calpain small subunit 1 belongs. CSS1 acts by regulating the proteolytic activity of calpain an enzyme complex critical for cellular signaling cytoskeletal remodeling and apoptosis. The small subunit plays an important role within the calpain complex facilitating the proper folding and activation of the catalytic subunit. This process ensures that calpain conforms to appropriate physiological conditions to exert its regulatory functions.

Pathways

The involvement of Calpain small subunit 1 in intracellular signaling pathways is significant. CSS1 plays a role in the calcium-dependent proteolytic pathway helping modulate the activity of proteins such as calpastatin the endogenous inhibitor of calpains. Calpain activity impacts many pathways including the cell cycle and apoptotic pathways. Through these pathways CSS1 contributes to cell proliferation differentiation and programmed cell death highlighting its multifunctional role in cellular homeostasis.

Calpain small subunit 1 has a notable association with certain pathological conditions. Dysregulation of calpain activity due to alterations in CSS1 can contribute to neurodegenerative diseases like Alzheimer's disease and muscular disorders such as limb-girdle muscular dystrophy. In Alzheimer's disease abnormal calpain activation affects tau protein processing linking CSS1 indirectly with tau-related pathologies. Moreover in muscular disorders calpain plays a role in muscle fiber degradation and repair where anomalies in CSS1 may impair muscle integrity and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity).
See full target information CAPNS1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 12:e025867 PubMed36789858

2023

Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Kurt D Marshall,Paula J Klutho,Lihui Song,Rajika Roy,Maike Krenz,Christopher P Baines

Environmental health perspectives 127:77003 PubMed31274337

2019

Use of a Mouse Model and Human Umbilical Vein Endothelial Cells to Investigate the Effect of Arsenic Exposure on Vascular Endothelial Function and the Associated Role of Calpains.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Cai,Yanqing Zhang,Yutian Zhang,Xiaofeng Miao,Shu Li,Hui Yang,Qinjie Ling,Peter R Hoffmann,Zhi Huang

Oncology letters 17:564-570 PubMed30655802

2019

Capn4 regulates migration and invasion of ovarian carcinoma cells via targeting osteopontin-mediated PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Chen,Gang Wang,Yingmei Wang,Xiaoli Gao,Kan Wang,Jie Li,Fengxia Xue

Journal of innate immunity 8:97-107 PubMed26228026

2015

Receptor-Interacting Protein 140 Orchestrates the Dynamics of Macrophage M1/M2 Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wei Lin,Bomi Lee,Pu-Ste Liu,Li-Na Wei

Biochimica et biophysica acta 1822:1783-95 PubMed22885154

2012

Intravitreal injection or topical eye-drop application of a μ-calpain C2L domain peptide protects against photoreceptor cell death in Royal College of Surgeons' rats, a model of retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Taku Ozaki,Mitsuru Nakazawa,Tetsuro Yamashita,Hiroyuki Sorimachi,Shoji Hata,Hiroshi Tomita,Hitomi Isago,Ayaka Baba,Sei-Ichi Ishiguro

The Journal of biological chemistry 285:40624-34 PubMed20947498

2010

Annexin 2 regulates endothelial morphogenesis by controlling AKT activation and junctional integrity.

Applications

WB

Species

Human

Shih-Chi Su,Steve A Maxwell,Kayla J Bayless

Journal of cell science 123:51-61 PubMed20016066

2009

Integrin activation by Fam38A uses a novel mechanism of R-Ras targeting to the endoplasmic reticulum.

Applications

WB

Species

Human

Brian J McHugh,Robert Buttery,Yatish Lad,Stephen Banks,Christopher Haslett,Tariq Sethi

Biochimica et biophysica acta 1793:1848-59 PubMed19833151

2009

Mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria.

Applications

WB

Species

Rat

Taku Ozaki,Tetsuro Yamashita,Sei-Ichi Ishiguro
View all publications
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Product promise

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