AB92333

Anti-Calpain small subunit 1 antibody [EPR3324]

RabMAb
Recombinant
KO Validated
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(12 Publications)

Rabbit Recombinant Monoclonal Calpain small subunit 1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.

View Alternative Names

CAPN4, CAPNS, CAPNS1, Calpain small subunit 1, CSS1, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calcium-dependent protease small subunit 1, Calpain regulatory subunit, CANP small subunit, CDPS

4 Images

Flow Cytometry (Intracellular) - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

Overlay histogram showing HeLa cells stained with ab92333 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92333, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

Immunohistochemistry staining of Calpain small subunit 1 in formalin-fixed paraffin-embedded Human kidney tissue using 1/100 ab92333.

Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

Lab

Western blot - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : Calpain small subunit 1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : A431 whole cell lysate (20 μg)
Lane 4 : HEK293 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab92333 observed at 28 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab92333 was shown to recognize Calpain small subunit 1 when Calpain small subunit 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Calpain small subunit 1 knockout samples were subjected to SDS-PAGE. ab92333 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Calpain small subunit 1 antibody [EPR3324] (ab92333)

Predicted band size: 28 kDa

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Unknown

Western blot - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

All lanes:

Western blot - Anti-Calpain small subunit 1 antibody [EPR3324] (ab92333) at 1/2000 dilution

Lane 1:

T-47D cell lysate at 10 µg

Lane 2:

Fetal brain tissue lysate at 10 µg

Lane 3:

Fetal spleen tissue lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Carrier free

Anti-Calpain small subunit 1 antibody [EPR3324] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3324

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain small subunit 1 also known as CSS1 or CAPNS1 is a small protein with a molecular mass of approximately 28 kDa. CSS1 forms an essential part of the calpain protease system which includes both the catalytic large and regulatory small subunits. CSS1 is important for the stability and function of the calpain holoenzyme. It expresses in various tissues including brain muscle and kidney suggesting its widespread functional role in cellular processes.

Biological function summary

Several cellular processes involve the calpain protease system to which Calpain small subunit 1 belongs. CSS1 acts by regulating the proteolytic activity of calpain an enzyme complex critical for cellular signaling cytoskeletal remodeling and apoptosis. The small subunit plays an important role within the calpain complex facilitating the proper folding and activation of the catalytic subunit. This process ensures that calpain conforms to appropriate physiological conditions to exert its regulatory functions.

Pathways

The involvement of Calpain small subunit 1 in intracellular signaling pathways is significant. CSS1 plays a role in the calcium-dependent proteolytic pathway helping modulate the activity of proteins such as calpastatin the endogenous inhibitor of calpains. Calpain activity impacts many pathways including the cell cycle and apoptotic pathways. Through these pathways CSS1 contributes to cell proliferation differentiation and programmed cell death highlighting its multifunctional role in cellular homeostasis.

Calpain small subunit 1 has a notable association with certain pathological conditions. Dysregulation of calpain activity due to alterations in CSS1 can contribute to neurodegenerative diseases like Alzheimer's disease and muscular disorders such as limb-girdle muscular dystrophy. In Alzheimer's disease abnormal calpain activation affects tau protein processing linking CSS1 indirectly with tau-related pathologies. Moreover in muscular disorders calpain plays a role in muscle fiber degradation and repair where anomalies in CSS1 may impair muscle integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Essential for embryonic development (By similarity).

See full target information CAPNS1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:516-526 PubMed40681898

2025

Pathology-oriented multiplexing enables integrative disease mapping.

Applications

Unspecified application

Species

Unspecified reactive species

Malte Kuehl,Yusuke Okabayashi,Milagros N Wong,Lukas Gernhold,Gabriele Gut,Nico Kaiser,Maria Schwerk,Stefanie K Gräfe,Frank Y Ma,Jovan Tanevski,Philipp S L Schäfer,Sam Mezher,Jacobo Sarabia Del Castillo,Thiago Goldbeck-Strieder,Olga Zolotareva,Michael Hartung,Fernando M Delgado Chaves,Lukas Klinkert,Ann-Christin Gnirck,Marc Spehr,David Fleck,Mehdi Joodaki,Victor Parra,Mina Shaigan,Martin Diebold,Marco Prinz,Jennifer Kranz,Johan M Kux,Fabian Braun,Oliver Kretz,Hui Wu,Florian Grahammer,Sven Heins,Marina Zimmermann,Fabian Haas,Dominik Kylies,Nicola Wanner,Jan Czogalla,Bernhard Dumoulin,Nikolay Zolotarev,Maja Lindenmeyer,Pall Karlson,Jens R Nyengaard,Marcial Sebode,Sören Weidemann,Thorsten Wiech,Hermann-Josef Groene,Nicola M Tomas,Catherine Meyer-Schwesinger,Christoph Kuppe,Rafael Kramann,Alexandre Karras,Patrick Bruneval,Pierre-Louis Tharaux,Diego Pastene,Benito Yard,Jennifer A Schaub,Phillip J McCown,Laura Pyle,Ye Ji Choi,Takashi Yokoo,Jan Baumbach,Pablo J Sáez,Ivan Costa,Jan-Eric Turner,Jeffrey B Hodgin,Julio Saez-Rodriguez,Tobias B Huber,Petter Bjornstad,Matthias Kretzler,Olivia Lenoir,David J Nikolic-Paterson,Lucas Pelkmans,Stefan Bonn,Victor G Puelles

Frontiers in cellular neuroscience 18:1344853 PubMed38515790

2024

Cell-free fat extract promotes axon regeneration and retinal ganglion cells survival in traumatic optic neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyu Sun,Di Chen,Tao Dai,Ziyou Yu,Hui Xie,Xiangsheng Wang,Wenjie Zhang

Genetics in medicine open 1:100811 PubMed38230350

2024

Biallelic variants in the calpain regulatory subunit cause pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Alex V Postma,Christina K Rapp,Katrin Knoflach,Alexander E Volk,Johannes R Lemke,Maximilian Ackermann,Nicolas Regamey,Philipp Latzin,Lucas Celant,Samara M A Jansen,Harm J Bogaard,Aho Ilgun,Mariëlle Alders,Karin Y van Spaendonck-Zwarts,Danny Jonigk,Christoph Klein,Stefan Gräf,Christian Kubisch,Arjan C Houweling,Matthias Griese

The Biochemical journal 478:3539-3553 PubMed34524407

2021

Calpain-2 specifically cleaves Junctophilin-2 at the same site as Calpain-1 but with less efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxi Wang,Grace Ciampa,Dong Zheng,Qian Shi,Biyi Chen,E Dale Abel,Tianqing Peng,Duane D Hall,Long-Sheng Song

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:701-709 PubMed34118141

2021

MiR-124 protects against cognitive dysfunction induced by sevoflurane anesthesia in vivo and in vitro through targeting calpain small subunit 1 via NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zijun Zhao,Li Ma,Yishuai Li,Qi Zhang,Ying Wang,Yanlei Tai,Qiujun Wang

Cell reports 34:108887 PubMed33761363

2021

Gasdermin D mediates the maturation and release of IL-1α downstream of inflammasomes.

Applications

Unspecified application

Species

Unspecified reactive species

Kohsuke Tsuchiya,Shoko Hosojima,Hideki Hara,Hiroko Kushiyama,Mamunur Rashid Mahib,Takeshi Kinoshita,Takashi Suda

Endocrine-related cancer 25:879-891 PubMed29895528

2018

Proteomics study of serum exosomes from papillary thyroid cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Luo,Shaohua Zhan,Wenchao Xia,Liang Huang,Wei Ge,Tianxiao Wang

Endocrinology 156:3239-52 PubMed26110916

2015

GH-Releasing Hormone Promotes Survival and Prevents TNF-α-Induced Apoptosis and Atrophy in C2C12 Myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Davide Gallo,Iacopo Gesmundo,Letizia Trovato,Giulia Pera,Eleonora Gargantini,Marco Alessandro Minetto,Ezio Ghigo,Riccarda Granata

Biochimica et biophysica acta 1852:1796-804 PubMed26071646

2015

Crosstalk between calpain activation and TGF-β1 augments collagen-I synthesis in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Zhi Li,Peng-Cheng Cai,Lin-Jie Song,Li-Ling Zhou,Qian Zhang,Shan-Shan Rao,Yu Xia,Fei Xiang,Jian-Bao Xin,Peter A Greer,Huan-Zhong Shi,Yunchao Su,Wan-Li Ma,Hong Ye

Cell death & disease 6:e1599 PubMed25590809

2015

NAMPT inhibition sensitizes pancreatic adenocarcinoma cells to tumor-selective, PAR-independent metabolic catastrophe and cell death induced by β-lapachone.

Applications

Unspecified application

Species

Unspecified reactive species

Z Moore,G Chakrabarti,X Luo,A Ali,Z Hu,F J Fattah,R Vemireddy,R J DeBerardinis,R A Brekken,D A Boothman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calpain small subunit 1 antibody [EPR3324]

Flow Cytometry (Intracellular) - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

Western blot - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

Western blot - Anti-Calpain small subunit 1 antibody [EPR3324] (AB92333)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise