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AB28252

Anti-Calpastatin antibody

3

(4 Reviews)

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(9 Publications)

Rabbit Polyclonal Calpastatin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Calpastatin.

View Alternative Names

Calpastatin, Calpain inhibitor, Sperm BS-17 component, CAST

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Calpastatin antibody (AB28252)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calpastatin antibody (AB28252)

ICC/IF image of ab28252 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28252, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human Calpastatin. The exact immunogen used to generate this antibody is proprietary information.

P20810

Specificity

ab28252 recognises Calpastatin aminoterminal end (Domain L) testis isoform

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpastatin is an endogenous inhibitor of calpain proteases with popular alternate names including CAST. The mass of calpastatin is approximately 70 kDa. It is expressed in various tissues throughout the body such as the brain heart and muscle. Calpastatin regulates the activity of the calpain family of cysteine proteases by binding to their catalytic domains. By modulating calpain activity calpastatin plays a role in several cellular processes including cytoskeletal remodeling and signal transduction.
Biological function summary

Calpastatin regulates intracellular protein degradation by inhibiting calpain activity. This inhibition prevents uncontrolled proteolysis of specific substrate proteins. Calpastatin is not part of a larger protein complex but interacts independently with multiple isoforms of calpain. It helps maintain cellular homeostasis by influencing autophagy apoptosis and cell migration integrating signals that drive various physiological processes.

Pathways

Calpain activity modulation through calpastatin takes place in several key biological pathways including apoptosis and the Wnt signaling pathway. Calpain-mediated cleavage affects cell survival and death by modifying Bcl-2 family proteins. In the Wnt signaling pathway calpastatin regulates calpain which influences β-catenin degradation therefore affecting cell proliferation and differentiation pathways. These interactions highlight calpastatin’s central role in managing fundamental cellular events.

Uncontrolled calpain activity due to insufficient calpastatin levels relates to neurodegenerative diseases such as Alzheimer's disease and muscular dystrophies. In Alzheimer's disease reduced calpastatin levels may lead to excessive calpain activity resulting in tau protein hyperphosphorylation and neurofibrillary tangles. Calpastatin-related calpain dysregulation also contributes to muscle cell degeneration seen in muscular dystrophies by affecting structural proteins like dystrophin. These connections highlight the critical balance that calpastatin maintains in cellular processes pertinent to human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
See full target information Calpastatin
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 21:124 PubMed33552245

2021

Inhibition of mitochondrial and cytosolic calpain attenuates atrophy in myotubes co-cultured with colon carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xianliang Zeng,Li Zhao,Sizeng Chen,Xiantao Li

Scientific reports 10:15185 PubMed32938992

2020

Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Popova,Anna Ulanova,Yulia Gritsyna,Nikolay Salmov,Vadim Rogachevsky,Gulnara Mikhailova,Alexander Bobylev,Liya Bobyleva,Yana Yutskevich,Oleg Morenkov,Nadezda Zakharova,Ivan Vikhlyantsev

Cell death & disease 11:487 PubMed32587237

2020

Calpain system is altered in survival motor neuron-reduced cells from in vitro and in vivo spinal muscular atrophy models.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra de la Fuente,Alba Sansa,Iván Hidalgo,Nuria Vivancos,Ricardo Romero-Guevara,Ana Garcera,Rosa M Soler

Experimental and therapeutic medicine 19:2433-2440 PubMed32226486

2020

Calpastatin peptide attenuates early brain injury following experimental subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Teng,Yanxin Yin,Jia Guo,Ming Jiang

Toxicology and applied pharmacology 380:114700 PubMed31398423

2019

Arsenic impairs GLUT1 trafficking through the inhibition of the calpain system in lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Pánico,Adriana Juárez-Nájera,Emilio Iturriaga-Goyon,Patricia Ostrosky-Wegman,Ana María Salazar

Oncotarget 8:72872-72885 PubMed29069832

2017

Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xianliang Zeng,Sizeng Chen,Yang Yang,Zhao Ke

Oncology reports 37:1601-1610 PubMed28112357

2017

Calpain inhibitors ameliorate muscle wasting in a cachectic mouse model bearing CT26 colorectal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Yu Lin,Si-Zeng Chen

Journal of animal science 91:3155-67 PubMed23798514

2013

The effects of Capn1 gene inactivation on skeletal muscle growth, development, and atrophy, and the compensatory role of other proteolytic systems.

Applications

Unspecified application

Species

Unspecified reactive species

C M Kemp,W T Oliver,T L Wheeler,A H Chishti,M Koohmaraie

Journal of applied physiology (Bethesda, Md. : 198 112:1839-46 PubMed22461440

2012

Aerobic exercise training upregulates skeletal muscle calpain and ubiquitin-proteasome systems in healthy mice.

Applications

Unspecified application

Species

Unspecified reactive species

Telma F Cunha,Jose B N Moreira,Nathalie A Paixão,Juliane C Campos,Alex W A Monteiro,Aline V N Bacurau,Carlos R Bueno,Julio C B Ferreira,Patricia C Brum
View all publications
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