AB101684

Anti-Calpastatin antibody [SP82]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Calpastatin antibody. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Calpastatin, Calpain inhibitor, Sperm BS-17 component, CAST

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpastatin antibody [SP82] (AB101684)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpastatin antibody [SP82] (AB101684)

Immunohistochemical analysis of Calpastatin in formalin-fixed, paraffin-embedded Human breast carcinoma using ab101684 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Calpastatin antibody [SP82] (AB101684)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calpastatin antibody [SP82] (AB101684)

Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling Calpastatin with purified ab101684. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-Calpastatin antibody [SP82] (AB101684)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Calpastatin antibody [SP82] (AB101684)

Flow cytometry analysis of HaCaT (human skin keratinocyte) labeling Calpastatin with purified ab101684 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpastatin antibody [SP82] (AB101684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Calpastatin with Purified ab101684 at 1/100 dilution (0.59 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Calpastatin antibody [SP82] (AB101684)

Overlay histogram showing HeLa cells stained with ab101684 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab101684) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Carrier free

Anti-Calpastatin antibody [SP82] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP82

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Incubate for 30 minutes at RT. Antigen retrieval is recommended: boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpastatin is an endogenous inhibitor of calpain proteases with popular alternate names including CAST. The mass of calpastatin is approximately 70 kDa. It is expressed in various tissues throughout the body such as the brain heart and muscle. Calpastatin regulates the activity of the calpain family of cysteine proteases by binding to their catalytic domains. By modulating calpain activity calpastatin plays a role in several cellular processes including cytoskeletal remodeling and signal transduction.

Biological function summary

Calpastatin regulates intracellular protein degradation by inhibiting calpain activity. This inhibition prevents uncontrolled proteolysis of specific substrate proteins. Calpastatin is not part of a larger protein complex but interacts independently with multiple isoforms of calpain. It helps maintain cellular homeostasis by influencing autophagy apoptosis and cell migration integrating signals that drive various physiological processes.

Pathways

Calpain activity modulation through calpastatin takes place in several key biological pathways including apoptosis and the Wnt signaling pathway. Calpain-mediated cleavage affects cell survival and death by modifying Bcl-2 family proteins. In the Wnt signaling pathway calpastatin regulates calpain which influences β-catenin degradation therefore affecting cell proliferation and differentiation pathways. These interactions highlight calpastatin’s central role in managing fundamental cellular events.

Uncontrolled calpain activity due to insufficient calpastatin levels relates to neurodegenerative diseases such as Alzheimer's disease and muscular dystrophies. In Alzheimer's disease reduced calpastatin levels may lead to excessive calpain activity resulting in tau protein hyperphosphorylation and neurofibrillary tangles. Calpastatin-related calpain dysregulation also contributes to muscle cell degeneration seen in muscular dystrophies by affecting structural proteins like dystrophin. These connections highlight the critical balance that calpastatin maintains in cellular processes pertinent to human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

See full target information Calpastatin

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:822 PubMed39537605

2024

SPINK13 acts as a tumor suppressor in hepatocellular carcinoma by inhibiting Akt phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhi Lun,Jie Sun,Ling Wei,Ben Liu,Zhixue Li,Wen Dong,Wenqi Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calpastatin antibody [SP82]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpastatin antibody [SP82] (AB101684)

Immunocytochemistry/ Immunofluorescence - Anti-Calpastatin antibody [SP82] (AB101684)

Flow Cytometry (Intracellular) - Anti-Calpastatin antibody [SP82] (AB101684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpastatin antibody [SP82] (AB101684)

Flow Cytometry (Intracellular) - Anti-Calpastatin antibody [SP82] (AB101684)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise