Mouse Multiclonal Calponin 1 antibody. Suitable for ICC, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Tissue preparation containing CNN1 protein.
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
ICC | IHC-P | |
---|---|---|
Human | Tested | Tested |
Rat | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1-2 µg/mL | Notes (Primary incubation for 30 minutes at RT). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1-2 µg/mL | Notes (Primary incubation for 30 minutes at RT). |
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Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
Calponin-1, Basic calponin, CNN1
Mouse Multiclonal Calponin 1 antibody. Suitable for ICC, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Tissue preparation containing CNN1 protein.
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Purified from Bioreactor Concentrate by Protein A/G.
What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Calponin 1 also known as CNN1 or calponin-a is a 34-kilodalton protein that plays an important role in muscle contraction. Found mainly in smooth muscle tissues calponin 1 interacts with actin and myosin modulating their interactions and contributing to muscle contraction mechanics. It inhibits the ATPase activity of myosin which affects the contractile capacity of smooth muscle. These interactions are important for maintaining the proper function and tone of vascular and visceral smooth muscles.
Calponin 1 acts as a regulatory protein in smooth muscle contraction by stabilizing actin filaments and modulating their interaction with other contractile proteins. It forms part of the thin filament-associated complex where it functions to control the conformational state of actin. This complex includes other proteins such as tropomyosin and troponin which together regulate the calcium-sensitive interactions between actin and myosin. The regulatory ability of calponin 1 contributes to the fine-tuning of smooth muscle contraction and relaxation processes.
Calponin 1 plays a role in the RhoA/ROCK signaling pathway and the calcium-sensitive contractile pathway. These pathways are integral to smooth muscle contraction regulation and involve proteins such as Rho kinase (ROCK) and myosin light chain kinase (MLCK). Calponin 1 contributes to the modulation of the phosphorylation status of myosin light chains influencing the contractile response to various stimuli. This points to its involvement in the physiological response mechanism to vasoconstrictive agents.
Calponin 1 is linked to cardiovascular diseases like hypertension and vascular disorders. Its regulatory functions in smooth muscle contraction can influence vascular tone and blood pressure connecting it to conditions like atherosclerosis. Moreover calponin 1 interacts with proteins such as filamin and α-actinin which are also implicated in muscle contraction-related disorders. Researchers investigate these interactions to understand their contributions to the pathogenesis of such diseases.
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Immunocytochemistry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Calponin 1 with ab233854 at 2 μg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
Formalin-fixed, paraffin-embedded human uterus tissue stained for Calponin 1 using ab233854 at 1 μg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Calponin 1 using ab233854 at 1 μg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat uterus tissue stained for Calponin 1 using ab233854 at 1 μg/ml in immunohistochemical analysis.
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