Mouse Multiclonal Calponin 1 antibody. Carrier free. Suitable for ICC, IHC-P and reacts with Human, Rat samples. Immunogen corresponding to Tissue preparation containing CNN1 protein.
Images
![Immunocytochemistry - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (AB212306), expandable thumbnail](https://content.abcam.com/products/images/calponin-1-antibody-cnn1-832-calp-bsa-and-azide-free-ab212306--immunocytochemistry-img81007.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (AB212306)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (AB212306), expandable thumbnail](https://content.abcam.com/products/images/calponin-1-antibody-cnn1-832-calp-bsa-and-azide-free-ab212306--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104275.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (AB212306)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (AB212306), expandable thumbnail](https://content.abcam.com/products/images/calponin-1-antibody-cnn1-832-calp-bsa-and-azide-free-ab212306--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137943.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (AB212306)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (AB212306), expandable thumbnail](https://content.abcam.com/products/images/calponin-1-antibody-cnn1-832-calp-bsa-and-azide-free-ab212306--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137628.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (AB212306)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- Tissue preparation containing CNN1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P51911
Reactivity data
Select an application| ICC | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Expected | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.5-1 µg/mL | Notes (Primary incubation for 30 minutes at RT). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 0.5-1 µg/mL | Notes (Primary incubation for 30 minutes at RT). |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
Alternative names
Calponin-1, Basic calponin, CNN1
Recommended products
Mouse Multiclonal Calponin 1 antibody. Carrier free. Suitable for ICC, IHC-P and reacts with Human, Rat samples. Immunogen corresponding to Tissue preparation containing CNN1 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- Tissue preparation containing CNN1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P51911
- Carrier free
- Yes
- Clone number
- CNN1/832 + CALP
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
ab212306 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
CNN1/832: IgG1 kappa. CALP: IgG1 kappa.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calponin 1 also known as CNN1 or calponin-a is a 34-kilodalton protein that plays an important role in muscle contraction. Found mainly in smooth muscle tissues calponin 1 interacts with actin and myosin modulating their interactions and contributing to muscle contraction mechanics. It inhibits the ATPase activity of myosin which affects the contractile capacity of smooth muscle. These interactions are important for maintaining the proper function and tone of vascular and visceral smooth muscles.
- Biological function summary
Calponin 1 acts as a regulatory protein in smooth muscle contraction by stabilizing actin filaments and modulating their interaction with other contractile proteins. It forms part of the thin filament-associated complex where it functions to control the conformational state of actin. This complex includes other proteins such as tropomyosin and troponin which together regulate the calcium-sensitive interactions between actin and myosin. The regulatory ability of calponin 1 contributes to the fine-tuning of smooth muscle contraction and relaxation processes.
- Pathways
Calponin 1 plays a role in the RhoA/ROCK signaling pathway and the calcium-sensitive contractile pathway. These pathways are integral to smooth muscle contraction regulation and involve proteins such as Rho kinase (ROCK) and myosin light chain kinase (MLCK). Calponin 1 contributes to the modulation of the phosphorylation status of myosin light chains influencing the contractile response to various stimuli. This points to its involvement in the physiological response mechanism to vasoconstrictive agents.
- Associated diseases and disorders
Calponin 1 is linked to cardiovascular diseases like hypertension and vascular disorders. Its regulatory functions in smooth muscle contraction can influence vascular tone and blood pressure connecting it to conditions like atherosclerosis. Moreover calponin 1 interacts with proteins such as filamin and α-actinin which are also implicated in muscle contraction-related disorders. Researchers investigate these interactions to understand their contributions to the pathogenesis of such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
![Immunocytochemistry - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306), expandable thumbnail](https://content.abcam.com/products/images/calponin-1-antibody-cnn1-832-calp-bsa-and-azide-free-ab212306--immunocytochemistry-img81007.jpg "Immunocytochemistry - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)")
Immunocytochemistry - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)
Immunocytochemistry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Calponin 1 with ab212306 at 2 μg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306), expandable thumbnail](https://content.abcam.com/products/images/calponin-1-antibody-cnn1-832-calp-bsa-and-azide-free-ab212306--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104275.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat uterus tissue labeling Calponin 1 with ab212306 at 1 μg/ml.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306), expandable thumbnail](https://content.abcam.com/products/images/calponin-1-antibody-cnn1-832-calp-bsa-and-azide-free-ab212306--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137943.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling Calponin 1 with ab212306 at 1 μg/ml.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306), expandable thumbnail](https://content.abcam.com/products/images/calponin-1-antibody-cnn1-832-calp-bsa-and-azide-free-ab212306--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137628.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [CNN1/832 + CALP] - BSA and Azide free (ab212306)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling Calponin 1 with ab212306 at 1 μg/ml.
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