Name: Anti-Calponin 1 antibody [EP798Y]
SKU: ab46794
Rating: 5 (20 reviews)

Anti-Calponin 1 antibody [EP798Y] (ab46794) is a rabbit monoclonal antibody that is used to detect Calponin 1 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Pig, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 250 publications

-Over 250 publications

Images

Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (AB46794), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	WB	ICC/IF	IHC-P
Human	Not recommended	Tested	Tested	Tested
Mouse	Not recommended	Tested	Tested	Tested
Rat	Not recommended	Tested	Expected	Tested
Pig	Not recommended	Tested	Expected	Expected
Sheep	Not recommended	Predicted	Predicted	Predicted

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Pig	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -
Species Human	Dilution info -	Notes -
Species Sheep	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes For unpurified use at 1/20,000.
Species Rat	Dilution info 1/5000	Notes For unpurified use at 1/20,000.
Species Pig	Dilution info 1/5000	Notes For unpurified use at 1/20,000.
Species Human	Dilution info 1/5000	Notes For unpurified use at 1/20,000.

Predicted
Predicted

Species	Dilution info	Notes
Species Sheep	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes For unpurified use at 1/100 - 1/250.
Species Human	Dilution info 1/500	Notes For unpurified use at 1/100 - 1/250.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Pig	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Sheep	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Pig	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Sheep	Dilution info -	Notes -

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

See full target information CNN1

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Alternative names

Calponin-1, Basic calponin, CNN1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP798Y
Purification technique: Affinity purification Protein A
Dissociation constant: 1.73 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Calponin 1 antibody [EP798Y] (ab46794) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Pig, Rat samples.

Recommended positive controls

ICC/IF: C2C12 and HeLa cells; Pig and mouse aortic smooth muscle cells.IHC-P: Rat lung tissue; Mouse cardiac muscle tissue; Human lung carcinoma, kidney, lung, tonsil, uterus, smooth muscle and skeletal muscle tissues.WB: Human bladder lysates, Pig heart lysates, Mouse bladder lysates and Rat bladder lysates.

Trusted by the scientific community

Anti-Calponin 1 [EP798Y] (ab46794) was first used in a scientific publication in 2007 and has been cited over 250 times in peer-reviewed journals.

Reviewed by scientists

Anti-Calponin 1 [EP798Y] (ab46794) has over 15 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products

We have a range of other formats of antibody clone [EP798Y] also available for your convenience:
ab46794, Alexa Fluor® 647 - ab197640, Carrier free - ab216651

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Calponin 1 also known as CNN1 or calponin-a is a 34-kilodalton protein that plays an important role in muscle contraction. Found mainly in smooth muscle tissues calponin 1 interacts with actin and myosin modulating their interactions and contributing to muscle contraction mechanics. It inhibits the ATPase activity of myosin which affects the contractile capacity of smooth muscle. These interactions are important for maintaining the proper function and tone of vascular and visceral smooth muscles.

Biological function summary

Calponin 1 acts as a regulatory protein in smooth muscle contraction by stabilizing actin filaments and modulating their interaction with other contractile proteins. It forms part of the thin filament-associated complex where it functions to control the conformational state of actin. This complex includes other proteins such as tropomyosin and troponin which together regulate the calcium-sensitive interactions between actin and myosin. The regulatory ability of calponin 1 contributes to the fine-tuning of smooth muscle contraction and relaxation processes.

Pathways

Calponin 1 plays a role in the RhoA/ROCK signaling pathway and the calcium-sensitive contractile pathway. These pathways are integral to smooth muscle contraction regulation and involve proteins such as Rho kinase (ROCK) and myosin light chain kinase (MLCK). Calponin 1 contributes to the modulation of the phosphorylation status of myosin light chains influencing the contractile response to various stimuli. This points to its involvement in the physiological response mechanism to vasoconstrictive agents.

Associated diseases and disorders

Calponin 1 is linked to cardiovascular diseases like hypertension and vascular disorders. Its regulatory functions in smooth muscle contraction can influence vascular tone and blood pressure connecting it to conditions like atherosclerosis. Moreover calponin 1 interacts with proteins such as filamin and α-actinin which are also implicated in muscle contraction-related disorders. Researchers investigate these interactions to understand their contributions to the pathogenesis of such diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

20 product images

Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Paraformadehyde-fixed, 0.25% Triton X-100 permeabilized mouse thoracic aortic smooth muscle cells labeling Calponin 1 using ab46794 at 1/100 dilution in ICC/IF, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/400 dilution.
1.5% BSA used used as blocking agent for 30 minutes at 25°C. Incubated with primary antibody for 24 hours at 4°C.
VSMCs were seeded to 35-mm plates in a low density avoiding overlapping of cells. After fixation, VSMCs were treated with 0.25% Triton X-100 for 20 minutes.
Image courtesy of an anonymous customer review.
Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
All lanes: Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/20000 dilution
All lanes: Whole tissue lysate prepared from bovine lymph vessels at 10 µg
Secondary
All lanes: HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 33 kDa
Observed band size: 34 kDa
Exposure time: 1min
Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/50000 dilution
Lane 1: Human bladder lysates at 15 µg
Lane 2: Mouse bladder lysates at 15 µg
Lane 3: Rat bladder lysates at 15 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 33 kDa
Image from Herington et al PLoS One. 2015 Nov 24;10(11):e0143243. doi: 10.1371/journal.pone.0143243. eCollection 2015. Fig 1.
Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Representative photomicrograph of mouse UT-myo cells (Left panel) and murine uterine myometrium (Right panel) stained with smooth muscle cell markers, alpha-SMA (red) and ab46794 (green) and DAPI (blue). UT-myo cells and whole-mount uterine tissue were collected from day 19 of mouse pregnancy. The placenta and embryo were removed from whole-mount tissue sections.
For full details please see paper.
Torrado M et al. Targeted gene-silencing reveals the functional significance of myocardin signaling in the failing heart. PLoS One 6:e26392 (2011). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Calponin 1 Western blot staining using rabbit Anti-Calponin 1 antibody
ab46794 was used to stain Calponin 1 in Neonatal piglets injected with Dox (Failing LV myocardium) and PBS (Non-Failing LV myocardium). At the protein level a more than twofold increase in Calponin 1 was observed in Dox-injected animals compared to controls (PBS).
All lanes: Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Lanes 1 - 2: Non-Failing pig LV-myocardium tissue lysate
Lanes 3 - 4: Failing pig LV-myocardium tissue lysate injected with DOX
Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunocytochemistry/ Immunofluorescence analysis of C2C12 (Mouse myoblasts myoblast) cells labeling Calponin 1 with purified ab46794 at 1/500 dilution. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody.
PBS instead of the primary antibody was used as the negative control (inset).
Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/5000 dilution
All lanes: Pig heart lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.
PBS instead of the primary antibody was used as the negative control (inset).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling Calponin 1 with purified ab46794 at a 1:1000 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.
PBS instead of the primary antibody was used as the negative control (inset).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Immunohistochemical staining of paraffin-embedded human smooth muscle using unpurified ab46794 at 1/100 dilution
This image is courtesy of a customer review submitted by Jordan Carbary.
Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 staining Calponin 1 in porcine aortic smooth muscle cells by Immunocytochemistry/ Immunofluorescence.
The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100. Samples were then incubated with primary antibody at 1/50 for 1 hour at 25°C. The secondary antibody used was Goat Anti-Rabbit IgG H&L (FITC) ab6717 Goat polyclonal to Rabbit IgG - H&L (FITC) (green) used at a 1/400 dilution.
Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794)
All lanes: Western blot - Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/20000 dilution
All lanes: Human bladder lysate at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Calponin 1 antibody [EP798Y] (ab46794)
ICC/IF image of unpurifiedab46794 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
Cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46794, 5μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight^® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing positive staining in normal kidney vessels tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing positive staining in normal lung vessel tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing positive staining in normal tonsil vessel tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing positive staining in normal uterus tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [EP798Y] (ab46794)
Unpurified ab46794 showing negative staining in skeletal muscle tissue.
OI-RD Scanning - Anti-Calponin 1 antibody [EP798Y] (ab46794)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.