AB227667

Anti-Calponin 1 antibody [SP193] - C-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Calponin 1 antibody. C-terminal. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

Calponin-1, Basic calponin, CNN1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

Formalin-fixed, paraffin-embedded human colon tissue stained for Calponin 1 with ab227667 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Calponin 1 with ab227667 at 1/100 dilution (1.26 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling Calponin 1 with ab227667 at 1/100 dilution (1.26 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling Calponin 1 with ab227667 at 1/100 dilution (1.26 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Carrier free

Anti-Calponin 1 antibody [SP193] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP193

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calponin 1 also known as CNN1 or calponin-a is a 34-kilodalton protein that plays an important role in muscle contraction. Found mainly in smooth muscle tissues calponin 1 interacts with actin and myosin modulating their interactions and contributing to muscle contraction mechanics. It inhibits the ATPase activity of myosin which affects the contractile capacity of smooth muscle. These interactions are important for maintaining the proper function and tone of vascular and visceral smooth muscles.

Biological function summary

Calponin 1 acts as a regulatory protein in smooth muscle contraction by stabilizing actin filaments and modulating their interaction with other contractile proteins. It forms part of the thin filament-associated complex where it functions to control the conformational state of actin. This complex includes other proteins such as tropomyosin and troponin which together regulate the calcium-sensitive interactions between actin and myosin. The regulatory ability of calponin 1 contributes to the fine-tuning of smooth muscle contraction and relaxation processes.

Pathways

Calponin 1 plays a role in the RhoA/ROCK signaling pathway and the calcium-sensitive contractile pathway. These pathways are integral to smooth muscle contraction regulation and involve proteins such as Rho kinase (ROCK) and myosin light chain kinase (MLCK). Calponin 1 contributes to the modulation of the phosphorylation status of myosin light chains influencing the contractile response to various stimuli. This points to its involvement in the physiological response mechanism to vasoconstrictive agents.

Calponin 1 is linked to cardiovascular diseases like hypertension and vascular disorders. Its regulatory functions in smooth muscle contraction can influence vascular tone and blood pressure connecting it to conditions like atherosclerosis. Moreover calponin 1 interacts with proteins such as filamin and α-actinin which are also implicated in muscle contraction-related disorders. Researchers investigate these interactions to understand their contributions to the pathogenesis of such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

See full target information CNN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7141 PubMed39164224

2024

CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guillem Pascual-Pasto,Brendan McIntyre,Margaret G Hines,Anna M Giudice,Laura Garcia-Gerique,Jennifer Hoffmann,Pamela Mishra,Stephanie Matlaga,Simona Lombardi,Rawan Shraim,Patrick M Schürch,Mark Yarmarkovich,Ted J Hofmann,Fatemeh Alikarami,Daniel Martinez,Matthew Tsang,Luis Gil-de-Gómez,Timothy T Spear,Kathrin M Bernt,Adam J Wolpaw,Dimiter S Dimitrov,Wei Li,Kristopher R Bosse

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calponin 1 antibody [SP193] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 1 antibody [SP193] - C-terminal (AB227667)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise