Goat Polyclonal Calponin 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CNN2 aa 250 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CNN2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99439
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Gorilla | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.50000-3.75000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.03000-0.10000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Target data
Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Alternative names
Calponin-2, Neutral calponin, CNN2
Recommended products
Goat Polyclonal Calponin 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CNN2 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CNN2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99439
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with Calponin 3.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calponin 2 also known as CNN2 or basic calponin is an actin-binding protein with a molecular weight of approximately 33 kDa. It binds to thin filaments in a calcium-dependent manner stabilizing these structures within the cell. Calponin 2 predominantly expresses in smooth muscle tissues and various non-muscle cells where it influences cellular tension and mechanical processes such as contraction and motility. Its ability to interact with actin makes it important for modulating the dynamic behavior of the cytoskeleton.
- Biological function summary
Calponin 2 plays a role in regulating smooth muscle contraction and modulating cellular motility. In smooth muscle cells it is not firm component of a larger regulatory complex or tightly bounded to a specific protein partner but acts individually to mediate these biological actions. It also participates in non-muscle cell activities where it assists in stabilizing actin structures. Such activity is important for the maintenance of cellular architecture and function across various cell types.
- Pathways
Calponin 2 is intimately connected to the regulation of the RhoA/Rho kinase pathway and the actin cytoskeleton remodeling processes. This pathway is important for cellular motility adhesion and contraction as it involves interplay with actin. Calponin 2 interacts with proteins like tropomyosin and myosin linking its mechanical effects on actin filaments to broader cellular functions. Together these interactions contribute to the precise modulation of cellular behaviors essential for tissue formation and repair.
- Associated diseases and disorders
Calponin 2 associates with conditions such as asthma and vascular diseases. In asthma altered expression or function of Calponin 2 within smooth muscle cells can affect bronchial contractions leading to symptoms exacerbation. Similarly in vascular diseases defects in Calponin 2 function could impair smooth muscle contractility affecting blood vessel function. Its expression and interaction with myosin and tropomyosin in these disorders highlight its importance in maintaining smooth muscle cell functionality and contractile health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 2 antibody (ab129331)
ab129331, at 2.5 μg/ml, staining Calponin 2 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.
Western blot - Anti-Calponin 2 antibody (ab129331)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-Calponin 2 antibody (ab129331) at 0.03 µg/mL
All lanes: HepG2 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Downloads
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