AB129331

Anti-Calponin 2 antibody

Name: Anti-Calponin 2 antibody (ab129331) | Abcam
Brand: Abcam Limited
SKU: AB129331

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(1 Publication)

Goat Polyclonal Calponin 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CNN2 aa 250 to C-terminus.

View Alternative Names

Calponin-2, Neutral calponin, CNN2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 2 antibody (AB129331)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 2 antibody (AB129331)

ab129331, at 2.5 μg/ml, staining Calponin 2 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Unknown

Western blot - Anti-Calponin 2 antibody (AB129331)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Calponin 2 antibody (ab129331) at 0.03 µg/mL

All lanes:

HepG2 lysate in RIPA buffer at 35 µg

Predicted band size: 34 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CNN2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99439

Specificity

This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with Calponin 3.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calponin 2 also known as CNN2 or basic calponin is an actin-binding protein with a molecular weight of approximately 33 kDa. It binds to thin filaments in a calcium-dependent manner stabilizing these structures within the cell. Calponin 2 predominantly expresses in smooth muscle tissues and various non-muscle cells where it influences cellular tension and mechanical processes such as contraction and motility. Its ability to interact with actin makes it important for modulating the dynamic behavior of the cytoskeleton.

Biological function summary

Calponin 2 plays a role in regulating smooth muscle contraction and modulating cellular motility. In smooth muscle cells it is not firm component of a larger regulatory complex or tightly bounded to a specific protein partner but acts individually to mediate these biological actions. It also participates in non-muscle cell activities where it assists in stabilizing actin structures. Such activity is important for the maintenance of cellular architecture and function across various cell types.

Pathways

Calponin 2 is intimately connected to the regulation of the RhoA/Rho kinase pathway and the actin cytoskeleton remodeling processes. This pathway is important for cellular motility adhesion and contraction as it involves interplay with actin. Calponin 2 interacts with proteins like tropomyosin and myosin linking its mechanical effects on actin filaments to broader cellular functions. Together these interactions contribute to the precise modulation of cellular behaviors essential for tissue formation and repair.

Calponin 2 associates with conditions such as asthma and vascular diseases. In asthma altered expression or function of Calponin 2 within smooth muscle cells can affect bronchial contractions leading to symptoms exacerbation. Similarly in vascular diseases defects in Calponin 2 function could impair smooth muscle contractility affecting blood vessel function. Its expression and interaction with myosin and tropomyosin in these disorders highlight its importance in maintaining smooth muscle cell functionality and contractile health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

See full target information CNN2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e18082 PubMed37539272

2023

Loss of Flot2 expression in deep cerebellar nuclei neurons of mice with Niemann-Pick disease type C.

Applications

Unspecified application

Species

Unspecified reactive species

Tsu-I Chen,Pei-Chun Hsu,Ni-Chung Lee,Yu-Han Liu,Hao-Chun Wang,Yen-Hsu Lu,Yin-Hsiu Chien,Wuh-Liang Hwu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calponin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 2 antibody (AB129331)

Western blot - Anti-Calponin 2 antibody (AB129331)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise