AB151427

Anti-Calponin 3 antibody

Name: Anti-Calponin 3 antibody (ab151427) | Abcam
Brand: Abcam Limited
SKU: AB151427
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Calponin 3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CNN3 aa 50 to C-terminus.

View Alternative Names

Calponin-3, CNN3

3 Images

Unknown

Western blot - Anti-Calponin 3 antibody (AB151427)

10% SDS-PAGE.

All lanes:

Western blot - Anti-Calponin 3 antibody (ab151427) at 1/5000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Lane 3:

HepG2 whole cell lysate at 30 µg

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-Calponin 3 antibody (AB151427)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-Calponin 3 antibody (ab151427) at 1/1000 dilution

Lane 1:

RMS-13 whole cell extract at 30 µg

Lane 2:

MCF-7 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

false

Unknown

Western blot - Anti-Calponin 3 antibody (AB151427)

10% SDS-PAGE.

All lanes:

Western blot - Anti-Calponin 3 antibody (ab151427) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CNN3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15417

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calponin 3 also known as CNN3 is a protein that regulates actin cytoskeleton dynamics. It is a smaller protein with a mass of approximately 37.5 kDa. Calponin 3 is expressed in various tissues including smooth muscle and certain non-muscle cells. This protein functions by interacting with actin filaments where it plays a role in stabilizing the cytoskeletal structure. CNN3 may also have similar functions to those of other members of the calponin family engaging in processes that influence cellular contractility and motility.

Biological function summary

The functions of calponin 3 extend to cellular processes such as migration and proliferation. It does not work alone but is a part of a network of proteins and may form complexes with other cytoskeletal components. Calponin 3 contributes to cellular activities by modulating actin filament interactions helping maintain cell shape and structural integrity under stress. As part of the calponin family its function resembles that of other family members which bind with actin and affect the contractile apparatus in cells.

Pathways

Calponin 3 integrates into the regulation of the actin cytoskeleton and cell migration pathways. It is associated with the Rho GTPase pathway influencing actin dynamics through these interactions. Another related pathway includes the calcium signaling pathway as calponin family members often show calcium-sensitive binding to actin. In these pathways calponin 3 interacts with proteins like tropomyosin and myosin which are critical to cytoskeletal organization and movement.

Calponin 3 has relevance to certain cancers and cardiovascular disorders. Changes in calponin 3 expression levels or function have been linked to tumor progression as its role in cell motility might influence cancer metastasis. The protein might also impact cardiovascular disease through its involvement in smooth muscle contraction regulation which could affect blood vessel function and contribute to hypertension. Interaction with myosin light chain kinase can further explain these effects as disruptions in this relationship can exacerbate disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

See full target information CNN3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e14802 PubMed38887185

2024

Identification and validation of COL6A1 as a novel target for tumor electric field therapy in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Chen,Yuyang Liu,Jinxin Lan,Hongyu Liu,Qingyun Tang,Ze Li,Xiaoguang Qiu,Wentao Hu,Jiaxin Xie,Yaping Feng,Lilin Qin,Xin Zhang,Jialin Liu,Ling Chen

Frontiers in physiology 12:659272 PubMed34305633

2021

Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling She,Cheng Li,Ting Jiang,Si Lei,Shanyao Zhou,Huacai Shi,Rui Chen

Aging 12:14037-14049 PubMed32667904

2020

Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Dai,Fei Luo,Rui Zhou,Qiang Zhou,Jianzhong Xu,Zehua Zhang,Jun Xiao,Lei Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calponin 3 antibody

Western blot - Anti-Calponin 3 antibody (AB151427)

Western blot - Anti-Calponin 3 antibody (AB151427)

Western blot - Anti-Calponin 3 antibody (AB151427)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise