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AB184132

Anti-Calponin 3 antibody

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(1 Publication)

Rabbit Polyclonal Calponin 3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CNN3 aa 1-300.

View Alternative Names

Calponin-3, CNN3

1 Images
Western blot - Anti-Calponin 3 antibody (AB184132)
  • WB

Supplier Data

Western blot - Anti-Calponin 3 antibody (AB184132)

10 % SDS-PAGE

All lanes:

Western blot - Anti-Calponin 3 antibody (ab184132) at 1/1000 dilution

Lane 1:

293T cell lysate at 30 µg

Lane 2:

HeLa cell lysate at 30 µg

Lane 3:

HepG2 cell lysate at 30 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human CNN3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q15417

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calponin 3 also known as CNN3 is a protein that regulates actin cytoskeleton dynamics. It is a smaller protein with a mass of approximately 37.5 kDa. Calponin 3 is expressed in various tissues including smooth muscle and certain non-muscle cells. This protein functions by interacting with actin filaments where it plays a role in stabilizing the cytoskeletal structure. CNN3 may also have similar functions to those of other members of the calponin family engaging in processes that influence cellular contractility and motility.
Biological function summary

The functions of calponin 3 extend to cellular processes such as migration and proliferation. It does not work alone but is a part of a network of proteins and may form complexes with other cytoskeletal components. Calponin 3 contributes to cellular activities by modulating actin filament interactions helping maintain cell shape and structural integrity under stress. As part of the calponin family its function resembles that of other family members which bind with actin and affect the contractile apparatus in cells.

Pathways

Calponin 3 integrates into the regulation of the actin cytoskeleton and cell migration pathways. It is associated with the Rho GTPase pathway influencing actin dynamics through these interactions. Another related pathway includes the calcium signaling pathway as calponin family members often show calcium-sensitive binding to actin. In these pathways calponin 3 interacts with proteins like tropomyosin and myosin which are critical to cytoskeletal organization and movement.

Calponin 3 has relevance to certain cancers and cardiovascular disorders. Changes in calponin 3 expression levels or function have been linked to tumor progression as its role in cell motility might influence cancer metastasis. The protein might also impact cardiovascular disease through its involvement in smooth muscle contraction regulation which could affect blood vessel function and contribute to hypertension. Interaction with myosin light chain kinase can further explain these effects as disruptions in this relationship can exacerbate disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
See full target information CNN3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:2164897 PubMed36405249

2022

GTPBP4: A New Therapeutic Target Gene Promotes Tumor Progression in Non-Small Cell Lung Cancer via EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Junlu Wu,Guofei Chen,Weiwei Wang,Yang Yang,Yi Yuan,Anquan Shang,Wenqiang Quan,Lixin Wang
View all publications
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Product promise

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