AB204365

Anti-Calponin 3 antibody - C-terminal

Name: Anti-Calponin 3 antibody - C-terminal (ab204365) | Abcam
Brand: Abcam Limited
SKU: AB204365

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(3 Publications)

Rabbit Polyclonal Calponin 3 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CNN3 aa 250 to C-terminus.

View Alternative Names

Calponin-3, CNN3

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunohistochemical analysis of human testis tissue labeling Calponin 3 in peritubular myoid cells with ab204365 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Calponin 3 antibody - C-terminal (AB204365)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling Calponin 3 in the cytosol and actin filaments with ab204365 at 2 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunohistochemical analysis of human placenta tissue labeling Calponin 3 in epithelial cells with ab204365 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunohistochemical analysis of human skeletal muscle tissue labeling Calponin 3 in myocytes with ab204365 at a 1/1000 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunohistochemical analysis of human kidney tissue labeling Calponin 3 in membrane and cytoplasm of cells in tubules with ab204365 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Calponin 3 antibody - C-terminal (AB204365)

All lanes:

Western blot - Anti-Calponin 3 antibody - C-terminal (ab204365) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CNN3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15417

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calponin 3 also known as CNN3 is a protein that regulates actin cytoskeleton dynamics. It is a smaller protein with a mass of approximately 37.5 kDa. Calponin 3 is expressed in various tissues including smooth muscle and certain non-muscle cells. This protein functions by interacting with actin filaments where it plays a role in stabilizing the cytoskeletal structure. CNN3 may also have similar functions to those of other members of the calponin family engaging in processes that influence cellular contractility and motility.

Biological function summary

The functions of calponin 3 extend to cellular processes such as migration and proliferation. It does not work alone but is a part of a network of proteins and may form complexes with other cytoskeletal components. Calponin 3 contributes to cellular activities by modulating actin filament interactions helping maintain cell shape and structural integrity under stress. As part of the calponin family its function resembles that of other family members which bind with actin and affect the contractile apparatus in cells.

Pathways

Calponin 3 integrates into the regulation of the actin cytoskeleton and cell migration pathways. It is associated with the Rho GTPase pathway influencing actin dynamics through these interactions. Another related pathway includes the calcium signaling pathway as calponin family members often show calcium-sensitive binding to actin. In these pathways calponin 3 interacts with proteins like tropomyosin and myosin which are critical to cytoskeletal organization and movement.

Calponin 3 has relevance to certain cancers and cardiovascular disorders. Changes in calponin 3 expression levels or function have been linked to tumor progression as its role in cell motility might influence cancer metastasis. The protein might also impact cardiovascular disease through its involvement in smooth muscle contraction regulation which could affect blood vessel function and contribute to hypertension. Interaction with myosin light chain kinase can further explain these effects as disruptions in this relationship can exacerbate disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

See full target information CNN3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112699 PubMed40520101

2025

KBG syndrome-associated protein ANKRD11 regulates SETD5 expression to modulate rRNA levels and translation.

Applications

Unspecified application

Species

Unspecified reactive species

Shoko Sashiyama,Tadashi Nakagawa,Makiko Nakagawa,Masaki Hosogane,Yasuaki Watanabe,Honoka Ashitomi,Kazusa Yamane,Norihiro Shibuya,Toshiro Moroishi,Keiko Nakayama,Toru Hosoi

Heliyon 10:e25234 PubMed38375306

2024

EPO regulates the differentiation and homing of bone marrow mesenchymal stem cells through Notch1/Jagged pathway to treat pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Chongyang Shen,Nianchi Wen,Yicen Han,Lu Guo

Cell reports 30:2820-2833.e3 PubMed32101754

2020

Sensing Actin Dynamics through Adherens Junctions.

Applications

Unspecified application

Species

Unspecified reactive species

Indrajyoti Indra,Regina B Troyanovsky,Lawrence Shapiro,Barry Honig,Sergey M Troyanovsky

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calponin 3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunocytochemistry/ Immunofluorescence - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (AB204365)

Western blot - Anti-Calponin 3 antibody - C-terminal (AB204365)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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