Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)

Rabbit Recombinant Monoclonal Calponin 3 antibody. Carrier free. Suitable for WB, IHC-P, IP, ICC/IF and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (AB314330), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended
Rat	Tested	Tested	Expected	Expected	Not recommended
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information CNN3

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Alternative names

Calponin-3, CNN3

Recommended products

Rabbit Recombinant Monoclonal Calponin 3 antibody. Carrier free. Suitable for WB, IHC-P, IP, ICC/IF and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28430-76
Purification technique: Affinity purification Protein A
Specificity: This antibody does not react with human Calponin 1 and Calponin 2.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab314330 is the carrier-free version of Anti-Calponin 3 antibody [EPR28430-76] ab314329.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Calponin 3 also known as CNN3 is a protein that regulates actin cytoskeleton dynamics. It is a smaller protein with a mass of approximately 37.5 kDa. Calponin 3 is expressed in various tissues including smooth muscle and certain non-muscle cells. This protein functions by interacting with actin filaments where it plays a role in stabilizing the cytoskeletal structure. CNN3 may also have similar functions to those of other members of the calponin family engaging in processes that influence cellular contractility and motility.

Biological function summary

The functions of calponin 3 extend to cellular processes such as migration and proliferation. It does not work alone but is a part of a network of proteins and may form complexes with other cytoskeletal components. Calponin 3 contributes to cellular activities by modulating actin filament interactions helping maintain cell shape and structural integrity under stress. As part of the calponin family its function resembles that of other family members which bind with actin and affect the contractile apparatus in cells.

Pathways

Calponin 3 integrates into the regulation of the actin cytoskeleton and cell migration pathways. It is associated with the Rho GTPase pathway influencing actin dynamics through these interactions. Another related pathway includes the calcium signaling pathway as calponin family members often show calcium-sensitive binding to actin. In these pathways calponin 3 interacts with proteins like tropomyosin and myosin which are critical to cytoskeletal organization and movement.

Associated diseases and disorders

Calponin 3 has relevance to certain cancers and cardiovascular disorders. Changes in calponin 3 expression levels or function have been linked to tumor progression as its role in cell motility might influence cancer metastasis. The protein might also impact cardiovascular disease through its involvement in smooth muscle contraction regulation which could affect blood vessel function and contribute to hypertension. Interaction with myosin light chain kinase can further explain these effects as disruptions in this relationship can exacerbate disease states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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13 product images

Immunocytochemistry/ Immunofluorescence - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Calponin 3 with Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Calponin 3 with Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. Negative control: MCF7 (PMID: 30911294).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Calponin 3 with Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/4000 (0.128 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human skeletal muscle. The section was incubated with Anti-Calponin 3 antibody [EPR28430-76] ab314329 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Calponin 3 with Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/4000 (0.128 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat colon. The section was incubated with Anti-Calponin 3 antibody [EPR28430-76] ab314329 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Calponin 3 with Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/4000 (0.128 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse colon. The section was incubated with Anti-Calponin 3 antibody [EPR28430-76] ab314329 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Calponin 3 with Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/4000 (0.128 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on chorionic villi of human placenta (PMID: 20861310). The section was incubated with Anti-Calponin 3 antibody [EPR28430-76] ab314329 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunoprecipitation - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Calponin 3 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2: Anti-Calponin 3 antibody [EPR28430-76] ab314329 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Calponin 3 antibody [EPR28430-76] ab314329 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
All lanes: Immunoprecipitation - Anti-Calponin 3 antibody [EPR28430-76] (Anti-Calponin 3 antibody [EPR28430-76] ab314329) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
Immunoprecipitation - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Calponin 3 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Calponin 3 antibody [EPR28430-76] ab314329 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-Calponin 3 antibody [EPR28430-76] ab314329 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Calponin 3 antibody [EPR28430-76] ab314329 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
All lanes: Immunoprecipitation - Anti-Calponin 3 antibody [EPR28430-76] (Anti-Calponin 3 antibody [EPR28430-76] ab314329) at 1/30 dilution
All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 24s
Western blot - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The recombinant proteins of Calponin 1, Calponin 2 and Calponin 3 were made in house.
This antibody does not react with human Calponin 1 and Calponin 2.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Calponin 3 antibody [EPR28430-76] (Anti-Calponin 3 antibody [EPR28430-76] ab314329) at 1/1000 dilution
Lane 1: His-tagged human Calponin 2 recombinant protein at 20 ng
Lane 2: His-tagged human Calponin 1 recombinant protein at 20 ng
Lane 3: His-tagged human Calponin 3 recombinant fragment at 20 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 17 kDa
Exposure time: 15s
Western blot - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Mouse skeletal muscle
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the bands around 25 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Calponin 3 antibody [EPR28430-76] (Anti-Calponin 3 antibody [EPR28430-76] ab314329) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 40 kDa
Exposure time: 26s
Western blot - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates in lane 2-5 were freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the bands around 25 kDa are unknown.
All lanes: Western blot - Anti-Calponin 3 antibody [EPR28430-76] (Anti-Calponin 3 antibody [EPR28430-76] ab314329) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat testis tissue lysate at 20 µg
Secondary
Lanes 1 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution
Lanes 1 - 5: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution
Observed band size: 40 kDa
Exposure time: 15s
Western blot - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In lanes 5-7, lysates were stored at -80℃ prior to Western Blotting. In lanes 1-4, lysate were freshly made and used for Western Blotting immediately to minimize protein degradation, showing a reduction in the intensity of bands between 15 kDa and 30 kDa.
All lanes: Western blot - Anti-Calponin 3 antibody [EPR28430-76] (Anti-Calponin 3 antibody [EPR28430-76] ab314329) at 1/1000 dilution
Lanes 1 and 6: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 2 and 5: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lanes 3 and 7: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 40 kDa
Exposure time: 10s
Western blot - Anti-Calponin 3 antibody [EPR28430-76] - BSA and Azide free (ab314330)
This data was developed using Anti-Calponin 3 antibody [EPR28430-76] ab314329, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: MCF7(PMID: 30911294).
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The identity of the bands around 25 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Calponin 3 antibody [EPR28430-76] (Anti-Calponin 3 antibody [EPR28430-76] ab314329) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 40 kDa
Exposure time: 15s