JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB315210

Anti-Calreticulin antibody [6C6]

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal Calreticulin antibody. Suitable for WB, ICC/IF and reacts with Human, Rat, African green monkey, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CALR.

View Alternative Names

CRTC, CALR, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody [6C6] (AB315210)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody [6C6] (AB315210)

Immunofluorescent analysis of of SH-SY5Y cells stained with ab315210, dilution 1 : 500 in green and costained with chicken pAb to lamin A/C, CPCA-LaminAC, dilution 1 : 2,000 in red. The blue is DAPI staining of nuclear DNA. The ab315210 antibody reveals granular staining of cytoplasm, while the lamin A/C antibody stains the nuclear lamina and membrane.

Western blot - Anti-Calreticulin antibody [6C6] (AB315210)
  • WB

Supplier Data

Western blot - Anti-Calreticulin antibody [6C6] (AB315210)

Western blot analysis of lysates of different cell lines using ab315210, dilution 1 : 2,000 : [1] protein standard, [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6, [7] COS-1 cells. A strong single band at about 50kDa corresponds to the calreticulin protein.

All lanes:

Western blot - Anti-Calreticulin antibody [6C6] (ab315210) at 1/2000 dilution

Lane 2:

NIH-3T3 cell lysate

Lane 3:

HEK293 cell lysate

Lane 4:

HeLa cell lysate

Lane 5:

SH-SY5Y cell lysate

Lane 6:

COS1 cell lysate

Observed band size: 50 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6C6

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, African green monkey, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CALR.

P27797

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7 - 8 Preservative: 0.03% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49.97% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed : 7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed : 11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
See full target information CALR
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB284624

Mouse Calreticulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Calreticulin ELISA Kit (AB284624)

  • 0
  • 0
Primary Antibodies

AB18256

Anti-HMGB1 antibody

KO Validated

Western blot - Anti-HMGB1 antibody (AB18256)

  • 5
  • 92
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com