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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal Calreticulin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CALR conjugated to Keyhole Limpet Haemocyanin.
IgG
Rabbit
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Expected | Tested |
Mouse | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60, CRTC, CALR
Rabbit Polyclonal Calreticulin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CALR conjugated to Keyhole Limpet Haemocyanin.
Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60, CRTC, CALR
IgG
Rabbit
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking buffer: 1.5% BSA.
Primary and secondary antibodies were incubated for 2 hours at RT.
All lanes: Western blot - Anti-Calreticulin antibody (AB223614) at 1/5000 dilution
Lane 1: A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg
Lane 2: A549 (human lung carcinoma cell line) cell lysate at 15 µg
Lane 3: HCT 116 (human colorectal carcinoma cell line) cell lysate at 15 µg
Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Lane 5: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg
Lane 6: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg
Lane 7: HL-60 (human promyelocytic leukemia cell line) cell lysate at 15 µg
Lane 8: HUVEC (human umbilical vein endothelial cell line) cell lysate at 15 µg
Lane 9: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg
Lane 10: MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg
Lane 11: PC-3 (human prostate adenocarcinoma cell line) cell lysate at 15 µg
Lane 12: T98G cell lysate at 15 µg
Lane 13: Rat brain lysate at 15 µg
All lanes: Donkey Anti-Rabbit IgG HRP
Predicted band size: 48 kDa
2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (yellow) using ab223614 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit R-PE at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.
(A) DAPI nuclear stain (B) ab223614 (C) Merge.
2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (green) using ab223614 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.
(A) DAPI nuclear stain (B) ab223614 (C) Merge.
Bouin's-fixed mouse backskin stained for Calreticulin (green) using ab223614 at 1/100 dilution in immunohistochemical analysis. Secondary: Goat anti-Rabbit FITC at 1/50 dilution for 1 hour at RT.
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