Anti-Calreticulin antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody (AB223614)

2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (yellow) using ab223614 at 1/100 dilution in ICC/IF. Secondary : Goat Anti-Rabbit R-PE at 1/200 dilution for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.

(A) DAPI nuclear stain (B) ab223614 (C) Merge.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody (AB223614)

2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (green) using ab223614 at 1/100 dilution in ICC/IF. Secondary : Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.

(A) DAPI nuclear stain (B) ab223614 (C) Merge.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calreticulin antibody (AB223614)

Bouin's-fixed mouse backskin stained for Calreticulin (green) using ab223614 at 1/100 dilution in immunohistochemical analysis. Secondary : Goat anti-Rabbit FITC at 1/50 dilution for 1 hour at RT.

• WB

Supplier Data

Western blot - Anti-Calreticulin antibody (AB223614)

Blocking buffer : 1.5% BSA.

Primary and secondary antibodies were incubated for 2 hours at RT.

All lanes:

Western blot - Anti-Calreticulin antibody (ab223614) at 1/5000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg

Lane 2:

A549 (human lung carcinoma cell line) cell lysate at 15 µg

Lane 3:

HCT 116 (human colorectal carcinoma cell line) cell lysate at 15 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

Lane 5:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg

Lane 6:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg

Lane 7:

HL-60 (human promyelocytic leukemia cell line) cell lysate at 15 µg

Lane 8:

HUVEC (human umbilical vein endothelial cell line) cell lysate at 15 µg

Lane 9:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

Lane 10:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg

Lane 11:

PC-3 (human prostate adenocarcinoma cell line) cell lysate at 15 µg

Lane 12:

T98G cell lysate at 15 µg

Lane 13:

Rat brain lysate at 15 µg

Secondary

All lanes:

Donkey Anti-Rabbit IgG HRP

Predicted band size: 48 kDa

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