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Rabbit Polyclonal Calreticulin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CALR conjugated to Keyhole Limpet Haemocyanin.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human CALR conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P27797

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Expected
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).

Alternative names

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Rabbit Polyclonal Calreticulin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CALR conjugated to Keyhole Limpet Haemocyanin.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human CALR conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P27797
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Calreticulin antibody (ab223614), expandable thumbnail

    Western blot - Anti-Calreticulin antibody (ab223614)

    Blocking buffer: 1.5% BSA.

    Primary and secondary antibodies were incubated for 2 hours at RT.

    All lanes: Western blot - Anti-Calreticulin antibody (AB223614) at 1/5000 dilution

    Lane 1: A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg

    Lane 2: A549 (human lung carcinoma cell line) cell lysate at 15 µg

    Lane 3: HCT 116 (human colorectal carcinoma cell line) cell lysate at 15 µg

    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

    Lane 5: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg

    Lane 6: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg

    Lane 7: HL-60 (human promyelocytic leukemia cell line) cell lysate at 15 µg

    Lane 8: HUVEC (human umbilical vein endothelial cell line) cell lysate at 15 µg

    Lane 9: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

    Lane 10: MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg

    Lane 11: PC-3 (human prostate adenocarcinoma cell line) cell lysate at 15 µg

    Lane 12: T98G cell lysate at 15 µg

    Lane 13: Rat brain lysate at 15 µg

    Secondary

    All lanes: Donkey Anti-Rabbit IgG HRP

    Predicted band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody (ab223614), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody (ab223614)

    2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (yellow) using ab223614 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit R-PE at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.

    (A) DAPI nuclear stain (B) ab223614 (C) Merge.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody (ab223614), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody (ab223614)

    2% Formaldehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Calreticulin (green) using ab223614 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours RT. Heat shocked at 42°C for 1h.

    (A) DAPI nuclear stain (B) ab223614 (C) Merge.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calreticulin antibody (ab223614), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calreticulin antibody (ab223614)

    Bouin's-fixed mouse backskin stained for Calreticulin (green) using ab223614 at 1/100 dilution in immunohistochemical analysis. Secondary: Goat anti-Rabbit FITC at 1/50 dilution for 1 hour at RT.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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