JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB265598

Anti-Calreticulin antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal Calreticulin antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CALR aa 350 to C-terminus.

View Alternative Names

CRTC, CALR, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60

2 Images
Immunoprecipitation - Anti-Calreticulin antibody (AB265598)
  • IP

Supplier Data

Immunoprecipitation - Anti-Calreticulin antibody (AB265598)

Calreticulin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265598 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab265598 at 0.1 μg/ml.

Lane 1 : ab265598 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 1 sec.

All lanes:

Immunoprecipitation - Anti-Calreticulin antibody (ab265598)

Predicted band size: 48 kDa

false

Western blot - Anti-Calreticulin antibody (AB265598)
  • WB

Supplier Data

Western blot - Anti-Calreticulin antibody (AB265598)

All lanes:

Western blot - Anti-Calreticulin antibody (ab265598) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 48 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CALR aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P27797

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed : 7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed : 11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
See full target information CALR
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB196158

Alexa Fluor® 488 Anti-Calreticulin antibody [EPR3924] - ER Marker

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB196158)

  • 3
  • 2
Primary Antibodies

AB196159

Alexa Fluor® 647 Anti-Calreticulin antibody [EPR3924] - ER Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB196159)

  • 5
  • 2
Primary Antibodies

AB92516

Anti-Calreticulin antibody [EPR3924] - ER Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calreticulin antibody [EPR3924] - ER Marker (AB92516)

  • 4
  • 6
Primary Antibodies

AB211962

Anti-Calreticulin antibody [EPR3924] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody [EPR3924] - Low endotoxin, Azide free (AB211962)

  • 0
  • 0
Primary Antibodies

AB210431

Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB210431)

  • 0
  • 0
Primary Antibodies

AB209577

PE Anti-Calreticulin antibody [EPR3924] - ER Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - PE Anti-Calreticulin antibody [EPR3924] - ER Marker (AB209577)

  • 5
  • 1

Complementary Products

Assay Kits

AB284624

Mouse Calreticulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Calreticulin ELISA Kit (AB284624)

  • 0
  • 0
Primary Antibodies

AB18256

Anti-HMGB1 antibody

KO Validated

Western blot - Anti-HMGB1 antibody (AB18256)

  • 5
  • 92
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB217344

Anti-CD8 alpha antibody [EPR21769]

RabMAb|Recombinant

Flow Cytometry - Anti-CD8 alpha antibody [EPR21769] (AB217344)

  • 5
  • 6
Primary Antibodies

AB154191

Anti-ERp57 antibody [EPR10678(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (AB154191)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com