Anti-Calreticulin antibody - ER Marker

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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (AB2907)

Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1 : 75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1 : 250) for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (1 : 120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (AB2907)

Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1 : 75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1 : 250) for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (1 : 120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (AB2907)

Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1 : 75) for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-rabbit IgG secondary antibody (1 : 250) for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (1 : 120) and nuclei (red) were stained with DRAQ5 (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (AB2907)

Immunofluorescence analysis of HepG2 cells, staining Calreticulin with ab2907.

Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin and blocked with 10% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% saponin) for 1 hour at 20°C. An AlexaFluor®647-conjugated donkey anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (AB2907)

Immunocytochemsitry/Immunofluorescence analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X for 30 minutes at room temperature. Cells were incubated with ab2907 (1 : 50) for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (1 : 250) for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (1 : 300) in PBS and incubated for 30 minutes. Nuclei (blue) were stained with Hoechst 33342 dye (1μg/mL). Images were taken at 20X magnification.

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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (AB2907)

Immunocytochemistry/Immunofluorescence analysis of Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1 : 75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (1 : 250) for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (1 : 300) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (AB2907)

ab2907 used at a 1/1000 dilution staining Calreticulin in HeLa cells by Immunocytochemistry/ Immunofluorescence.

HeLa cells were transfected overnight with empty vector or plasmids encoding the indicated IFITM3 constructs. Immunofluorescence with a-HA antibodies allowed IFITM3 visualization, and a-calreticulin staining allowed visualization of the ER. TOPRO-3 was used to visualize nuclei. Scale bars indicate 10 µm. UbΔ indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine.

Image from Yount JS et al, J Biol Chem. 2012 Jun 1;287(23):19631-41. Epub 2012 Apr 17, Fig 3. DOI 10.1074/jbc.M112.362095 June 1, 2012 The Journal of Biological Chemistry, 287, 19631-19641.

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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (AB2907)

Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labelling Calreticulin using ab2907.