Mouse Monoclonal Calretinin antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CALB2.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [1F5H1] (AB204990), expandable thumbnail](https://content.abcam.com/products/images/calretinin-antibody-1f5h1-ab204990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131533.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [1F5H1] (AB204990)")
![Western blot - Anti-Calretinin antibody [1F5H1] (AB204990), expandable thumbnail](https://content.abcam.com/products/images/calretinin-antibody-1f5h1-ab204990--western-blot-img54155.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calretinin antibody [1F5H1] (AB204990)")
![Flow Cytometry - Anti-Calretinin antibody [1F5H1] (AB204990), expandable thumbnail](https://content.abcam.com/products/images/calretinin-antibody-1f5h1-ab204990--flow-cytometry-img121675.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Calretinin antibody [1F5H1] (AB204990)")
![Western blot - Anti-Calretinin antibody [1F5H1] (AB204990), expandable thumbnail](https://content.abcam.com/products/images/calretinin-antibody-1f5h1-ab204990--western-blot-img151034.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calretinin antibody [1F5H1] (AB204990)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CALB2. Database link P22676
Consider this alternative
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/400 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Alternative names
CAB29, CALB2, Calretinin, CR, 29 kDa calbindin
Recommended products
Mouse Monoclonal Calretinin antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CALB2.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CALB2. Database link P22676
- Clone number
- 1F5H1
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [1F5H1] (ab204990), expandable thumbnail](https://content.abcam.com/products/images/calretinin-antibody-1f5h1-ab204990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131533.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [1F5H1] (ab204990)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [1F5H1] (ab204990)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Calretinin with ab204990 at 1/200 dilution with DAB staining.
![Western blot - Anti-Calretinin antibody [1F5H1] (ab204990), expandable thumbnail](https://content.abcam.com/products/images/calretinin-antibody-1f5h1-ab204990--western-blot-img54155.jpg "Western blot - Anti-Calretinin antibody [1F5H1] (ab204990)")
Western blot - Anti-Calretinin antibody [1F5H1] (ab204990)
Expected MWt is 57.5 kDa.
All lanes: Western blot - Anti-Calretinin antibody [1F5H1] (ab204990) at 1/500 dilution
All lanes: Human Calretinin (aa1-271) recombinant protein
Predicted band size: 31 kDa
![Flow Cytometry - Anti-Calretinin antibody [1F5H1] (ab204990), expandable thumbnail](https://content.abcam.com/products/images/calretinin-antibody-1f5h1-ab204990--flow-cytometry-img121675.jpg "Flow Cytometry - Anti-Calretinin antibody [1F5H1] (ab204990)")
Flow Cytometry - Anti-Calretinin antibody [1F5H1] (ab204990)
Flow cytometric analysis of A549 cells labeling Calretinin with ab204990 at 1/200 dilution (green), compared to a negative control (red).
![Western blot - Anti-Calretinin antibody [1F5H1] (ab204990), expandable thumbnail](https://content.abcam.com/products/images/calretinin-antibody-1f5h1-ab204990--western-blot-img151034.jpg "Western blot - Anti-Calretinin antibody [1F5H1] (ab204990)")
Western blot - Anti-Calretinin antibody [1F5H1] (ab204990)
All lanes: Western blot - Anti-Calretinin antibody [1F5H1] (ab204990) at 1/500 dilution
Lane 1: HEK293 cell lysate
Lane 2: Calretinin (aa1-271)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 31 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com