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AB244299

Anti-Calretinin antibody

5

(1 Review)

|

(6 Publications)

Rabbit Polyclonal Calretinin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CALB2 aa 100 to C-terminus.

View Alternative Names

CAB29, CALB2, Calretinin, CR, 29 kDa calbindin

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)

Formalin-fixed, paraffin-embedded human testis tissue stained for Calretinin with ab244299 in immunohistochemical analysis shows strong cytoplasmic and nuclear positivity in Leydig cells..

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Calretinin with ab244299 in immunohistochemical analysis shows strong cytoplasmic and nuclear positivity in cells in granular layer.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Calretinin with ab244299 in immunohistochemical analysis shows strong cytoplasmic positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody (AB244299)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody (AB244299)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling Calretinin using ab244299 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)

Formalin-fixed, paraffin-embedded mouse hippocampus tissue stained for Calretinin with ab244299 in immunohistochemical analysis shows selective immunoreactivity in the dentate gyrus neurons.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)

Formalin-fixed, paraffin-embedded mouse hindbrain tissue stained for Calretinin with ab244299 in immunohistochemical analysis cytoplasmic immunoreactivity in neurons of the pontine nucleus.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)

Formalin-fixed, paraffin-embedded mouse substantia nigra tissue stained for Calretinin with ab244299 in immunohistochemical analysis shows neuronal cell bodies and processes in pars compacta.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)

Formalin-fixed, paraffin-embedded mouse medulla tissue stained for Calretinin with ab244299 in immunohistochemical analysis shows positivity in the gigantocellular reticular nucleus neurons.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (AB244299)

Formalin-fixed, paraffin-embedded mouse hypothalamus tissue stained for Calretinin with ab244299 in immunohistochemical analysis shows immunoreactivity in neurons of the paraventricular nucleus.

Western blot - Anti-Calretinin antibody (AB244299)
  • WB

Supplier Data

Western blot - Anti-Calretinin antibody (AB244299)

All lanes:

Western blot - Anti-Calretinin antibody (ab244299) at 0.4 µg/mL

Lane 1:

U-2 OS (Human bone osteosarcoma epithelial cell line) transfected with control siRNA

Lane 2:

U-2 OS transfected with siRNA probe #1

Lane 3:

U-2 OS transfected with siRNA probe #2

Predicted band size: 31 kDa

false

Western blot - Anti-Calretinin antibody (AB244299)
  • WB

Supplier Data

Western blot - Anti-Calretinin antibody (AB244299)

All lanes:

Western blot - Anti-Calretinin antibody (ab244299) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CALB2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P22676

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-binding protein involved in calcium homeostasis and signal transduction. It plays a critical role in buffering intracellular calcium levels and modulating calcium-dependent signaling pathways (PubMed : 2001709). Predominantly expressed in specific neuronal populations, influences synaptic plasticity and neuronal excitability, contributing to learning and memory (By similarity). During embryonic development, it facilitates neuronal differentiation and maturation (By similarity).
See full target information CALB2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature 634:1150-1159 PubMed39358505

2024

CRISPR-Cas9 screens reveal regulators of ageing in neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tyson J Ruetz,Angela N Pogson,Chloe M Kashiwagi,Stephanie D Gagnon,Bhek Morton,Eric D Sun,Jeeyoon Na,Robin W Yeo,Dena S Leeman,David W Morgens,C Kimberly Tsui,Amy Li,Michael C Bassik,Anne Brunet

Life science alliance 7: PubMed38167610

2024

2',3' cyclic nucleotide 3' phosphodiesterase 1 functional isoform antagonizes HIV-1 particle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Shuntao Liang,Qin Zhang,Fang Wang,Shiwei Wang,Guoli Li,Dong Jiang,Hui Zeng

BMC research notes 16:246 PubMed37777793

2023

Affordable optical clearing and immunolabelling in mouse brain slices.

Applications

Unspecified application

Species

Unspecified reactive species

Phillip M Muza,Marta Pérez,Suzanna Noy,Miyu Kurosawa,Loukia Katsouri,Victor L J Tybulewicz,Elizabeth M C Fisher,Steven J West

iScience 26:106073 PubMed36818290

2023

Cognitive impairments in a Down syndrome model with abnormal hippocampal and prefrontal dynamics and cytoarchitecture.

Applications

Unspecified application

Species

Unspecified reactive species

Phillip M Muza,Daniel Bush,Marta Pérez-González,Ines Zouhair,Karen Cleverley,Miriam L Sopena,Rifdat Aoidi,Steven J West,Mark Good,Victor L J Tybulewicz,Matthew C Walker,Elizabeth M C Fisher,Pishan Chang

International journal of molecular sciences 23: PubMed36555318

2022

Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca Overload during Ischemia by Protecting the Population of GABAergic Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Elena G Varlamova,Egor Y Plotnikov,Egor A Turovsky

Translational psychiatry 12:24 PubMed35058425

2022

Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Hye M Hwang,Kazue Hashimoto-Torii
View all publications
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Product promise

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