Rabbit Polyclonal Calretinin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CALB2 aa 100 to C-terminus.
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Publications
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- iScience 26:1060732023Cognitive impairments in a Down syndrome model with abnormal hippocampal and prefrontal dynamics and cytoarchitecture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhillip M Muza,Daniel Bush,Marta Pérez-González,Ines Zouhair,Karen Cleverley,Miriam L Sopena,Rifdat Aoidi,Steven J West,Mark Good,Victor L J Tybulewicz,Matthew C Walker,Elizabeth M C Fisher,Pishan ChangPubMed 36818290
- International journal of molecular sciences 23:2022Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca Overload during Ischemia by Protecting the Population of GABAergic Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena G Varlamova,Egor Y Plotnikov,Egor A TurovskyPubMed 36555318
- Translational psychiatry 12:242022Activation of the anterior cingulate cortex ameliorates anxiety in a preclinical model of fetal alcohol spectrum disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye M Hwang,Kazue Hashimoto-ToriiPubMed 35058425
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CALB2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P22676
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000.00000 - 1/10000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/5000.00000 - 1/10000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Alternative names
CAB29, CALB2, Calretinin, CR, 29 kDa calbindin
Recommended products
Rabbit Polyclonal Calretinin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CALB2 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CALB2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P22676
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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11 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded human stomach tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded mouse hippocampus tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody (ab244299)
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling Calretinin using ab244299 at 4 μg/ml (green) in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded human testis tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-Calretinin antibody (ab244299)
All lanes: Western blot - Anti-Calretinin antibody (ab244299) at 0.4 µg/mL
Lane 1: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2: NBT-II (Rat cell line) cell lysate
Predicted band size: 31 kDa
Western blot - Anti-Calretinin antibody (ab244299)
All lanes: Western blot - Anti-Calretinin antibody (ab244299) at 0.4 µg/mL
Lane 1: U-2 OS (Human bone osteosarcoma epithelial cell line) transfected with control siRNA
Lane 2: U-2 OS transfected with siRNA probe #1
Lane 3: U-2 OS transfected with siRNA probe #2
Predicted band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded mouse hypothalamus tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded mouse medulla tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded mouse hindbrain tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody (ab244299)
Formalin-fixed, paraffin-embedded mouse substantia nigra tissue stained for Calretinin with ab244299 at a 1/50 dilution in immunohistochemical analysis.
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