Name: Anti-Calretinin antibody [EP1798]
SKU: ab92341
Rating: 4 (5 reviews)

Anti-Calretinin antibody [EP1798] (ab92341) is a rabbit monoclonal antibody that is used to detect Calretinin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications

-Over 20 publications

Related conjugates and formulations

ab210633
Conjugated
PE Anti-Calretinin antibody [EP1798]
ab232462
Carrier free
Anti-Calretinin antibody [EP1798] - BSA and Azide free
ab313189
Conjugated
Alexa Fluor® 555 Anti-Calretinin antibody [EP1798]
ab311707
Conjugated
Alexa Fluor® 594 Anti-Calretinin antibody [EP1798]
ab310883
Conjugated
APC Anti-Calretinin antibody [EP1798]
ab310975
Conjugated
Alexa Fluor® 488 Anti-Calretinin antibody [EP1798]
ab312982
Conjugated
Alexa Fluor® 568 Anti-Calretinin antibody [EP1798]
ab214244
Conjugated
Alexa Fluor® 647 Anti-Calretinin antibody [EP1798]
ab321750
Conjugated
Alexa Fluor® 750 Anti-Calretinin antibody [EP1798]

Images

Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody [EP1798] (AB92341), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Expected	Expected
Rat	Tested	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/4000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/4000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/4000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

See full target information CALB2

Function

Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Alternative names

CAB29, CALB2, Calretinin, CR, 29 kDa calbindin

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1798
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Calretinin antibody [EP1798] (ab92341) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Calretinin?

Anti-Calretinin [EP1798] (ab92341) specifically detects a band for Calretinin (UniProt: P22676) at a molecular weight of 29kDa.

Trusted by the scientific community

Anti-Calretinin [EP1798] (ab92341) was first used in a scientific publication in 2010 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EP1798] also available for your convenience:
ab92341, PE - ab210633, Alexa Fluor® 647 - ab214244, Carrier free - ab232462, APC - ab310883, Alexa Fluor® 488 - ab310975, Alexa Fluor® 594 - ab311707, Alexa Fluor® 568 - ab312982, Alexa Fluor® 555 - ab313189, Alexa Fluor® 750 - ab321750

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Immunocytochemistry/ Immunofluorescence - Anti-Calretinin antibody [EP1798] (ab92341)
Immunofluorecent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilsed SH-SY5Y (human neuroblastoma epithelial cell line) cells labelling Calretinin with ab92341 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line is observed. Counterstained with DAPI.
Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
All lanes: Western blot - Anti-Calretinin antibody [EP1798] (ab92341) at 1/1000 dilution
Lane 1: Mouse brain lysate at 20 µg
Lane 2: Rat brain lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Flow Cytometry (Intracellular) - Anti-Calretinin antibody [EP1798] (ab92341)
Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Calretinin with purified ab92341 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Western blot - Anti-Calretinin antibody [EP1798] (ab92341)
All lanes: Western blot - Anti-Calretinin antibody [EP1798] (ab92341) at 1/1000 dilution
All lanes: Human brain lysate at 15 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [EP1798] (ab92341)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human mesothelioma tissue sections labeling Calretinin with purified ab92341 at 1:4000 (0.034 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.