Rabbit Recombinant Monoclonal Calsequestrin 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--western-blot-img61479.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)")
![Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--western-blot-img61146.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48705.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90887.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)")
![Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--flow-cytometry-intracellular-img16339.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Tested | Not recommended | Expected |
| Rat | Tested | Not recommended | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/50000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/10000 - 1/50000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed:28895244). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (PubMed:28895244). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (PubMed:27185316).
Alternative names
CASQ, CASQ1, Calsequestrin-1, Calmitine
Recommended products
Rabbit Recombinant Monoclonal Calsequestrin 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15226(B)
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is not suitable for cell line testing in western blot.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--western-blot-img61479.jpg "Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)")
Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)
All lanes: Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/10000 dilution
All lanes: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 44 kDa
![Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--western-blot-img61146.jpg "Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)")
Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)
All lanes: Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/10000 dilution
Lane 1: Rat muscle tissue lysate at 20 µg
Lane 2: Mouse muscle tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 44 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48705.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90887.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--flow-cytometry-intracellular-img16339.jpg "Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)")
Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)
Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Calsequestrin 1with ab185220 at 1/240 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
![Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--western-blot-img416195.jpg "Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)")
Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)
Blocking and diluting buffer and concentrartion: 5% NFDM/TBST.
Exposure time: Lane 1: 30 seconds; Lane 2: 180 seconds.
CASQ1 is the major isoform in adult fast-twitch skeletal muscles, while CASQ2 is the only isoform in cardiac muscle and a minor isoform in adult slow-twitch skeletal muscle (PMID: 33288873).
Lane 1: Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/10000 dilution
Lane 2: Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/2000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 63 kDa
![Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220), expandable thumbnail](https://content.abcam.com/products/images/calsequestrin-1-antibody-epr15226b-ab185220--western-blot-img416195.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220)")
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