AB185220

Anti-Calsequestrin 1 antibody [EPR15226(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Calsequestrin 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

CASQ, CASQ1, Calsequestrin-1, Calmitine

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Calsequestrin 1with ab185220 at 1/240 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

All lanes:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/10000 dilution

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

Lab

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Blocking and diluting buffer and concentrartion : 5% NFDM/TBST.

Exposure time : Lane 1 : 30 seconds; Lane 2 : 180 seconds.

CASQ1 is the major isoform in adult fast-twitch skeletal muscles, while CASQ2 is the only isoform in cardiac muscle and a minor isoform in adult slow-twitch skeletal muscle (PMID : 33288873).

Lane 1:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/10000 dilution

Lane 2:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/2000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 63 kDa

false

Supplier Data

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

All lanes:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/10000 dilution

Lane 1:

Rat muscle tissue lysate at 20 µg

Lane 2:

Mouse muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

Carrier free

Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15226(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not suitable for cell line testing in western blot.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/240", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed : 28895244). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (PubMed : 28895244). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (PubMed : 27185316).

See full target information CASQ1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 17:e1009539 PubMed33914734

2021

Mapping the degradation pathway of a disease-linked aspartoacylase variant.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Gersing,Yong Wang,Martin Grønbæk-Thygesen,Caroline Kampmeyer,Lene Clausen,Martin Willemoës,Claes Andréasson,Amelie Stein,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calsequestrin 1 antibody [EPR15226(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (AB185220)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise