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AB250819

Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Calsequestrin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication.

View Alternative Names

CASQ, CASQ1, Calsequestrin-1, Calmitine

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Calsequestrin 1 with ab185220 at 1/240 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)
  • WB

Supplier Data

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (<a href='/en-us/products/primary-antibodies/calsequestrin-1-antibody-epr15226b-ab185220'>ab185220</a>) at 1/10000 dilution

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)
  • WB

Lab

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentrartion : 5% NFDM/TBST. Exposure time : Lane 1 : 30 seconds; Lane 2 : 180 seconds. CASQ1 is the major isoform in adult fast-twitch skeletal muscles, while CASQ2 is the only isoform in cardiac muscle and a minor isoform in adult slow-twitch skeletal muscle (PMID : 33288873).

Lane 1:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (<a href='/en-us/products/primary-antibodies/calsequestrin-1-antibody-epr15226b-ab185220'>ab185220</a>) at 1/10000 dilution

Lane 2:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (<a href='/en-us/products/primary-antibodies/calsequestrin-1-antibody-epr15226b-ab185220'>ab185220</a>) at 1/2000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 63 kDa

false

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)
  • WB

Supplier Data

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819)

This data was developed using ab185220, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (<a href='/en-us/products/primary-antibodies/calsequestrin-1-antibody-epr15226b-ab185220'>ab185220</a>) at 1/10000 dilution

Lane 1:

Rat muscle tissue lysate at 20 µg

Lane 2:

Mouse muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15226(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is not suitable for cell line testing in western blot.

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Complementary Products

Primary Antibodies

AB108289

Anti-Calsequestrin 2 antibody [EPR4227]

RabMAb|Recombinant

Western blot - Anti-Calsequestrin 2 antibody [EPR4227] (AB108289)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB2869

Anti-NCX1 antibody [C2C12]

BOND RX™ Validated

Immunohistochemistry (Frozen sections) - Anti-NCX1 antibody [C2C12] (AB2869)

  • 5
  • 4
Primary Antibodies

AB219626

Anti-Phospholamban antibody [EPR21897]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholamban antibody [EPR21897] (AB219626)

  • 4
  • 1
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Reactivity data

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Product details

ab250819 is the carrier-free version of ab185220.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed : 28895244). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (PubMed : 28895244). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (PubMed : 27185316).
See full target information CASQ1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com