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Rabbit Recombinant Monoclonal Calsequestrin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples.

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Images

Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819), expandable thumbnail
  • Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (AB250819), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Expected
Rat
Tested
Not recommended
Tested

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle (PubMed:28895244). Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca(2+) ions (PubMed:28895244). Regulates the release of lumenal Ca(2+) via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. Negatively regulates store-operated Ca(2+) entry (SOCE) activity (PubMed:27185316).

    Alternative names

    Calsequestrin-1, Calmitine, CASQ, CASQ1

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    Rabbit Recombinant Monoclonal Calsequestrin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples.

    Alternative names

    Calsequestrin-1, Calmitine, CASQ, CASQ1

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR15226(B)

    Purification technique

    Affinity purification Protein A

    Specificity

    This antibody is not suitable for cell line testing in western blot.

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Storage information

    Do Not Freeze

    Notes

    ab250819 is the carrier-free version of Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    6 product images

    • Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819), expandable thumbnail

      Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819)

      This data was developed using Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220, the same antibody clone in a different buffer formulation.

      All lanes: Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220) at 1/10000 dilution

      All lanes: Human skeletal muscle tissue lysate at 20 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 44 kDa

    • Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819), expandable thumbnail

      Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819)

      This data was developed using Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220, the same antibody clone in a different buffer formulation.

      All lanes: Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220) at 1/10000 dilution

      Lane 1: Rat muscle tissue lysate at 20 µg

      Lane 2: Mouse muscle tissue lysate at 20 µg

      Secondary

      All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 44 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819)

      This data was developed using Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Calsequestrin 1 with Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220 at 1/500 dilution. Counterstain: Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819)

      This data was developed using Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 1 with Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220 at 1/500 dilution. Counterstain: Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819), expandable thumbnail

      Flow Cytometry (Intracellular) - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819)

      This data was developed using Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220, the same antibody clone in a different buffer formulation.

      Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Calsequestrin 1 with Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220 at 1/240 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    • Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819), expandable thumbnail

      Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] - BSA and Azide free (ab250819)

      This data was developed using Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentrartion: 5% NFDM/TBST.

      Exposure time: Lane 1: 30 seconds; Lane 2: 180 seconds.

      CASQ1 is the major isoform in adult fast-twitch skeletal muscles, while CASQ2 is the only isoform in cardiac muscle and a minor isoform in adult slow-twitch skeletal muscle (PMID: 33288873).

      Lane 1: Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220) at 1/10000 dilution

      Lane 2: Western blot - Anti-Calsequestrin 1 antibody [EPR15226(B)] (Anti-Calsequestrin 1 antibody [EPR15226(B)] ab185220) at 1/2000 dilution

      Lane 1: Human skeletal muscle tissue lysate at 20 µg

      Lane 2: Human heart tissue lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Predicted band size: 44 kDa

      Observed band size: 63 kDa

      Blocking and diluting buffer and concentrartion: 5% NFDM/TBST.

      Exposure time: Lane 1: 30 seconds; Lane 2: 180 seconds.

      CASQ1 is the major isoform in adult fast-twitch skeletal muscles, while CASQ2 is the only isoform in cardiac muscle and a minor isoform in adult slow-twitch skeletal muscle (PMID: 33288873).

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com