Anti-Calsequestrin 1 antibody [EPR15227(B)]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15227(B)] (AB191564)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Calsequestrin 1 with ab191564 at a 1/250 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15227(B)] (AB191564)

Immunohistochemical analysis of paraffin-embedded mouse Skeletal muscle tissue labelling Calsequestrin 1 with ab191564 at 1/10000 dilution, followed LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse skeletal muscle. The section was incubated with ab191564 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15227(B)] (AB191564)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labelling Calsequestrin 1 with ab191564 at 1/10000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse colon. The section was incubated with ab191564 for 30 mins at room temperature. Counter stained with Hematoxylin

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 antibody [EPR15227(B)] (AB191564)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 1 with ab191564 at a 1/250 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Calsequestrin 1 antibody [EPR15227(B)] (AB191564)

All lanes:

Western blot - Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564) at 1/10000 dilution

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 63 kDa

true

• WB

Supplier Data

Western blot - Anti-Calsequestrin 1 antibody [EPR15227(B)] (AB191564)

All lanes:

Western blot - Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564) at 1/10000 dilution

Lane 1:

Mouse muscle tissue lysate at 10 µg

Lane 2:

Rat muscle tissue lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 63 kDa

true