AB108289

Anti-Calsequestrin 2 antibody [EPR4227]

RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal Calsequestrin 2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

Calsequestrin-2, CASQ2

1 Images

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Western blot - Anti-Calsequestrin 2 antibody [EPR4227] (AB108289)

All lanes:

Western blot - Anti-Calsequestrin 2 antibody [EPR4227] (ab108289) at 1/1000 dilution

Lane 1:

Human heart cell lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Rat heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 55 kDa

false

Carrier free

Anti-Calsequestrin 2 antibody [EPR4227] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4227

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.

See full target information CASQ2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 13:834220 PubMed35360237

2022

Aldosterone-Induced Sarco/Endoplasmic Reticulum Ca Pump Upregulation Counterbalances Ca1.2-Mediated Ca Influx in Mesenteric Arteries.

Applications

Unspecified application

Species

Unspecified reactive species

Rogelio Salazar-Enciso,Agustín Guerrero-Hernández,Ana M Gómez,Jean-Pierre Benitah,Angélica Rueda

JCI insight 7: PubMed34990403

2022

The Purkinje-myocardial junction is the anatomic origin of ventricular arrhythmia in CPVT.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Blackwell,Michela Faggioni,Matthew J Wleklinski,Nieves Gomez-Hurtado,Raghav Venkataraman,Chelsea E Gibbs,Franz J Baudenbacher,Shiaoching Gong,Glenn I Fishman,Patrick M Boyle,Karl Pfeifer,Bjorn C Knollmann

Annals of the New York Academy of Sciences 1478:63-74 PubMed32713021

2020

Protein expression profiles in murine ventricles modeling catecholaminergic polymorphic ventricular tachycardia: effects of genotype and sex.

Applications

Unspecified application

Species

Unspecified reactive species

Khalil Saadeh,Zakaria Achercouk,Ibrahim T Fazmin,Nakulan Nantha Kumar,Samantha C Salvage,Charlotte E Edling,Christopher L-H Huang,Kamalan Jeevaratnam

Applied physiology, nutrition, and metabolism = Ph 45:1049-1053 PubMed32379978

2020

Dietary nitrate does not alter cardiac function, calcium handling proteins, or SERCA activity in the left ventricle of healthy rats.

Applications

Unspecified application

Species

Unspecified reactive species

Aleah J Kirsh,Emma S Juracic,Heather L Petrick,Cynthia M F Monaco,Pierre-Andre Barbeau,A Russell Tupling,Graham P Holloway

Physiological reports 8:e14408 PubMed32342642

2020

Resting metabolic rate and skeletal muscle SERCA and Na /K ATPase activities are not affected by fish oil supplementation in healthy older adults.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Jannas-Vela,Shannon L Klingel,Daniel T Cervone,Kate A Wickham,George J F Heigenhauser,David M Mutch,Graham P Holloway,Lawrence L Spriet

Cellular physiology and biochemistry : internation 47:1167-1180 PubMed29913448

2018

Rapid Electrical Stimulation Increased Cardiac Apoptosis Through Disturbance of Calcium Homeostasis and Mitochondrial Dysfunction in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Le Geng,Zidun Wang,Chang Cui,Yue Zhu,Jiaojiao Shi,Jiaxian Wang,Minglong Chen

PloS one 12:e0184724 PubMed28886186

2017

The cardiac calsequestrin gene transcription is modulated at the promoter by NFAT and MEF-2 transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Estrada-Avilés,Gabriela Rodríguez,Angel Zarain-Herzberg

Circulation research 115:68-78 PubMed24759929

2014

EHD3-dependent endosome pathway regulates cardiac membrane excitability and physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Jerry Curran,Michael A Makara,Sean C Little,Hassan Musa,Bin Liu,Xiangqiong Wu,Iuliia Polina,Joseph S Alecusan,Patrick Wright,Jingdong Li,George E Billman,Penelope A Boyden,Sandor Gyorke,Hamid Band,Thomas J Hund,Peter J Mohler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Calsequestrin 2 antibody [EPR4227]

Western blot - Anti-Calsequestrin 2 antibody [EPR4227] (AB108289)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

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