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AB247652

Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free

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Rabbit Recombinant Monoclonal Calsequestrin 2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

Calsequestrin-2, CASQ2

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Calsequestrin 2 with Purified ab108381 at 1 : 250 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscles using unpurified ab108381 at a dilution of 1/100.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscles using unpurified ab108381 at a dilution of 1/100.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Unpurified ab108381 showing negative staining in Normal colon tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Unpurified ab108381 showing negative staining in Normal breast tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • WB

Lab

Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Calsequestrin 2 antibody [EPR4228] (<a href='/en-us/products/primary-antibodies/calsequestrin-2-antibody-epr4228-ab108381'>ab108381</a>) at 1/10000 dilution

Lane 1:

Human fetal heart lysates at 20 µg

Lane 2:

Human skeletal muscle lysates at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 55 kDa

false

Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • WB

Unknown

Western blot - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Calsequestrin 2 antibody [EPR4228] (<a href='/en-us/products/primary-antibodies/calsequestrin-2-antibody-epr4228-ab108381'>ab108381</a>) at 1/1000 dilution

Lane 1:

Human heart lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Predicted band size: 198 kDa,28 kDa,39 kDa,41 kDa,46 kDa,98 kDa

Observed band size: 55 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Unpurified ab108381 showing negative staining in Normal tonsil tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Unpurified ab108381 showing negative staining in Normal brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (AB247652)

This data was developed using ab108381, the same antibody clone in a different buffer formulation.

Unpurified ab108381 showing negative staining in Normal kidney tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4228

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247652 is the carrier-free version of ab108381.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.
See full target information CASQ2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com