Rabbit Recombinant Monoclonal Calumenin antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-Calumenin antibody [EPR9075] (AB137019), expandable thumbnail](https://content.abcam.com/products/images/calumenin-antibody-epr9075-ab137019--western-blot-img107364.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Calumenin antibody [EPR9075] (AB137019)")
![Flow Cytometry (Intracellular) - Anti-Calumenin antibody [EPR9075] (AB137019), expandable thumbnail](https://content.abcam.com/products/images/calumenin-antibody-epr9075-ab137019--flow-cytometry-intracellular-img139501.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Calumenin antibody [EPR9075] (AB137019)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (AB137019), expandable thumbnail](https://content.abcam.com/products/images/calumenin-antibody-epr9075-ab137019--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92282.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (AB137019)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (AB137019), expandable thumbnail](https://content.abcam.com/products/images/calumenin-antibody-epr9075-ab137019--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41553.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (AB137019)")
Publications
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- Frontiers in oncology 11:6839512021The Epithelial to Mesenchymal Transition Related Gene Calumenin Is an Adverse Prognostic Factor of Bladder Cancer Correlated With Tumor Microenvironment Remodeling, Gene Mutation, and Ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiHeng Du,WenHao Miao,Xiang Jiang,Jin Cao,Bo Wang,Yi Wang,Jiang Yu,XiZhi Wang,HaiTao LiuPubMed 34150647
- Frontiers in oncology 11:6206742021Identification and Validation of a Stromal EMT Related LncRNA Signature as a Potential Marker to Predict Bladder Cancer Outcome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiHeng Du,Bo Wang,Xiang Jiang,Jin Cao,Jiang Yu,Yi Wang,XiZhi Wang,HaiTao LiuPubMed 33747932
- Scientific reports 8:88382018MicroRNA-21 in cancer-associated fibroblasts supports lung adenocarcinoma progression.
- Oncotarget 6:25252-652015PERK/CHOP contributes to the CGK733-induced vesicular calcium sequestration which is accompanied by non-apoptotic cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufeng Wang,Yasuhiro Kuramitsu,Byron Baron,Takao Kitagawa,Junko Akada,Kazuhiro Tokuda,Dan Cui,Kazuyuki NakamuraPubMed 26259235
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity (By similarity).
Alternative names
Calumenin, Crocalbin, IEF SSP 9302, CALU
Recommended products
Rabbit Recombinant Monoclonal Calumenin antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR9075
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Calumenin antibody [EPR9075] (ab137019), expandable thumbnail](https://content.abcam.com/products/images/calumenin-antibody-epr9075-ab137019--western-blot-img107364.jpg "Western blot - Anti-Calumenin antibody [EPR9075] (ab137019)")
Western blot - Anti-Calumenin antibody [EPR9075] (ab137019)
All lanes: Western blot - Anti-Calumenin antibody [EPR9075] (ab137019) at 1/1000 dilution
Lane 1: Human placenta lysate at 10 µg
Lane 2: Jurkat lysate at 10 µg
Lane 3: 293T lysate at 10 µg
Lane 4: K562 lysate at 10 µg
Lane 5: HACAT lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
![Flow Cytometry (Intracellular) - Anti-Calumenin antibody [EPR9075] (ab137019), expandable thumbnail](https://content.abcam.com/products/images/calumenin-antibody-epr9075-ab137019--flow-cytometry-intracellular-img139501.jpg "Flow Cytometry (Intracellular) - Anti-Calumenin antibody [EPR9075] (ab137019)")
Flow Cytometry (Intracellular) - Anti-Calumenin antibody [EPR9075] (ab137019)
Overlay histogram showing JEG3 cells stained with ab137019 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137019, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (ab137019), expandable thumbnail](https://content.abcam.com/products/images/calumenin-antibody-epr9075-ab137019--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92282.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (ab137019)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (ab137019)
Immunohistochemical analysis of paraffin-embedded Human placanta tissue labelling Calumenin with ab137019 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (ab137019), expandable thumbnail](https://content.abcam.com/products/images/calumenin-antibody-epr9075-ab137019--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41553.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (ab137019)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (ab137019)
Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue labelling Calumenin with ab137019 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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