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AB137019

Anti-Calumenin antibody [EPR9075]

3

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal Calumenin antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Calumenin, Crocalbin, IEF SSP 9302, CALU

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (AB137019)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (AB137019)

Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue labelling Calumenin with ab137019 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (AB137019)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calumenin antibody [EPR9075] (AB137019)

Immunohistochemical analysis of paraffin-embedded Human placanta tissue labelling Calumenin with ab137019 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Calumenin antibody [EPR9075] (AB137019)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Calumenin antibody [EPR9075] (AB137019)

Overlay histogram showing JEG3 cells stained with ab137019 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137019, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-Calumenin antibody [EPR9075] (AB137019)
  • WB

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Western blot - Anti-Calumenin antibody [EPR9075] (AB137019)

All lanes:

Western blot - Anti-Calumenin antibody [EPR9075] (ab137019) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

K562 lysate at 10 µg

Lane 5:

HACAT lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9075

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Primary Antibodies

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Primary Antibodies

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Anti-14-3-3 gamma/YWHAG antibody [EPR9655(B)] - BSA and Azide free

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity (By similarity).
See full target information Calumenin
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 17:6743-6764 PubMed39345892

2024

Pan-Cancer Screening and Validation of CALU's Role in EMT Regulation and Tumor Microenvironment in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Liang Chen,Dan Hu,Tian-Zhu Chen,Si-Yu Shen,Chen-Fei Zhao,Cong Wang,Shi-Yuan Tong,Zhao Liu,Shao-Hua Lin,Li-Xia Jin,Yi-Bo He,Zhe-Zhong Zhang

Frontiers in oncology 11:683951 PubMed34150647

2021

The Epithelial to Mesenchymal Transition Related Gene Calumenin Is an Adverse Prognostic Factor of Bladder Cancer Correlated With Tumor Microenvironment Remodeling, Gene Mutation, and Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

YiHeng Du,WenHao Miao,Xiang Jiang,Jin Cao,Bo Wang,Yi Wang,Jiang Yu,XiZhi Wang,HaiTao Liu

Frontiers in oncology 11:620674 PubMed33747932

2021

Identification and Validation of a Stromal EMT Related LncRNA Signature as a Potential Marker to Predict Bladder Cancer Outcome.

Applications

Unspecified application

Species

Unspecified reactive species

YiHeng Du,Bo Wang,Xiang Jiang,Jin Cao,Jiang Yu,Yi Wang,XiZhi Wang,HaiTao Liu

Scientific reports 8:8838 PubMed29892003

2018

MicroRNA-21 in cancer-associated fibroblasts supports lung adenocarcinoma progression.

Applications

IHC-P

Species

Human

Akiko Kunita,Shigeki Morita,Tomoko U Irisa,Akiteru Goto,Toshiro Niki,Daiya Takai,Jun Nakajima,Masashi Fukayama

Oncotarget 6:25252-65 PubMed26259235

2015

PERK/CHOP contributes to the CGK733-induced vesicular calcium sequestration which is accompanied by non-apoptotic cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Wang,Yasuhiro Kuramitsu,Byron Baron,Takao Kitagawa,Junko Akada,Kazuhiro Tokuda,Dan Cui,Kazuyuki Nakamura
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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