Anti-CAMK1D antibody [EPR3536(2)]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CAMK1D antibody [EPR3536(2)] (AB172618)

Immunofluorescent analysis of 293T cells labeling CAMK1D with ab172618 at 1/50 dilution (red). DAPI nuclear staining (blue).

• WB

Supplier Data

Western blot - Anti-CAMK1D antibody [EPR3536(2)] (AB172618)

All lanes:

Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618) at 1/10000 dilution

Lane 1:

Human fetal forebrain tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Predicted band size: 43 kDa

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• WB

Lab

Western blot - Anti-CAMK1D antibody [EPR3536(2)] (AB172618)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CAMK1D knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HEK293 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab172618 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab172618 was shown to specifically react with CAMK1D when CAMK1D knockout samples were used. Wild-type and CAMK1D knockout samples were subjected to SDS-PAGE. ab172618 and ab18058 (loading control to Vinculin) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)

Predicted band size: 43 kDa

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• WB

Lab

Western blot - Anti-CAMK1D antibody [EPR3536(2)] (AB172618)

Western blot : Rabbit Monoclonal[EPR3536(2)] to CAMK1D ab172618 staining at 1/10000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 42 kDa in Wild-type A549 cell lysates with no signal observed at this size in CAMK1D knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618) at 1/10000 dilution

Lane 1:

Wild-type A549 at 10 µg

Lane 2:

CAMK1D knockout A549 at 10 µg

Lane 3:

HeLa at 10 µg

Lane 4:

K562 at 10 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa,76 kDa

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