AB320638

Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37]

20ul selling size
Recombinant
RabMAb
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal KCC2B phospho T287 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Mouse, Rat, Synthetic peptide samples.

View Alternative Names

CAM2, CAMK2, CAMKB, CAMK2B, Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta, CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMKD, CAMK2D, Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II subunit delta, CaMK-II subunit delta, CAMK, CAMK-II, CAMKG, CAMK2G, Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CaMK-II subunit gamma

7 Images

Supplier Data

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : placenta, spleen (PMID : 1321343).

The molecular weight observed is consistent with what has been described in the literature (PMID : 29615706). This antibody can recognize CaMKII beta, CaMKII alpha, CaMKII delta and CaMKII gamma. They have many isofroms and isoforms have different molecular weight.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (ab320638) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50-60 kDa,36 kDa

false

Exposure time: 37s

Supplier Data

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-CaMKII beta antibody [EPR28076-54] - (ab314897) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (ab320638) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate (untreated membrane) at 20 µg

Lane 2:

Human cerebellum tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50-60 kDa,60 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-CaMKII beta antibody [EPR28076-54] - (ab314897) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (ab320638) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-60 kDa,60 kDa

false

Exposure time: 8s

Supplier Data

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-CaMKII beta antibody [EPR28076-54] - (ab314897) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (ab320638) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Rat brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-60 kDa,60 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : placenta, spleen (PMID : 1321343).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1, 5 : 26 seconds; Lanes 2-4, 6-7 : 125 seconds

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (ab320638) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse placenta tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat cerebellum tissue lysate at 20 µg

Lane 7:

Rat placenta tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-60 kDa,36 kDa

false

Supplier Data

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-CaMKII beta antibody [EPR28076-54] - (ab314897) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (ab320638) at 1/1000 dilution

Lane 1:

293T cells transfected with a human CaMKII beta (T287A) expression vector containing a myc-His-tag® whole cell lysate (untreated membrane) at 10 µg

Lane 2:

293T cells transfected with a human wild-type CaMKII beta expression vector containing a myc-His-tag® whole cell lysate (untreated membrane) at 10 µg

Lane 3:

293T cells transfected with a human wild-type CaMKII beta expression vector containing a myc-His-tag® whole cell lysate (alkaline phosphatase treated membrane) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50-60 kDa,80 kDa

false

Exposure time: 15s

Supplier Data

Dot Blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Dot blot analysis of CAMK2B/ 2G/ 2D (phospho T287) + CAMK2A (phospho T286) using ab320638 at 1 : 1000 (0.526 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (ab320638) at 1/1000 dilution

Lane 1:

CaMKII beta (phospho T287) peptide

Lane 2:

CaMKII beta non-phospho peptide

Lane 3:

CaMKII alpha (phospho T286) peptide, CaMKII delta (phospho T287) peptide

Lane 4:

CaMKII gamma (phospho T287) peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Carrier free

Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28075-37

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CaMKII (Ca2+/calmodulin-dependent protein kinase II) family consists of four isoforms: CaMKII alpha CaMKII beta CaMKII delta and CaMKII gamma also known respectively as CAMK2A CAMK2B CAMK2D and CAMK2G. These are serine/threonine-specific protein kinases with a molecular weight of around 50 to 60 kDa. They play a role in the phosphorylation of various downstream targets. These kinases are found in many tissues but CaMKII alpha is mostly found in brain tissue CaMKII beta in muscle tissue whereas CaMKII delta and gamma show more widespread expression across other tissues. The functional unit of CaMKII is a holoenzyme which forms a dodecamer consisting of 12 subunits.

Biological function summary

CaMKII isoforms participate in critical calcium signaling processes. They act to decode the frequency of calcium oscillations in cells and are involved in synaptic plasticity and memory formation. CaMKII alpha for example is highly active in the postsynaptic density where it can form complexes with both NMDA and AMPA receptors suggesting a role in the regulation of synaptic strength. While not part of a permanent complex CaMKII enzymes can transiently bind to other proteins during signaling events.

Pathways

CaMKII isoforms play significant roles in the calcium/calmodulin signal transduction pathway and the MAPK (mitogen-activated protein kinase) signaling cascade. In the calcium/calmodulin pathway CaMKII acts to mediate calcium-dependent phosphorylation events that affect neuronal and cardiac functions. It interacts with other pathway proteins such as calmodulin and CREB (cAMP response element-binding protein). In the MAPK pathway CaMKII modulates signals that control cell growth differentiation and response to cellular stress interacting with proteins like MEK and ERK.

CaMKII isoforms have connections to neurodegenerative diseases such as Alzheimer's disease and to cardiac conditions like heart failure. In Alzheimer's disease altered CaMKII activity can affect synaptic function linking to proteins like amyloid-beta peptides. In cardiac disorders abnormal CaMKII activation contributes to disrupted calcium homeostasis and arrhythmia where it interacts with proteins such as RyR2 (ryanodine receptor 2). Understanding these interactions highlights CaMKII's significance in both brain and heart health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle (PubMed : 16690701). In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed : 29100089). Participates in the modulation of skeletal muscle function in response to exercise (PubMed : 16690701). In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity). Phosphorylates reticulophagy regulator RETREG1 at 'Ser-151' under endoplasmic reticulum stress conditions which enhances RETREG1 oligomerization and its membrane scission and reticulophagy activity (PubMed : 31930741).

See full target information CAMK2B phospho T287

Additional targets

CAMK2A phospho T286,,CAMK2G phospho T287

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37]

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Dot Blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (AB320638)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise