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Rabbit Recombinant Monoclonal KCC2B phospho T287 antibody. Carrier free. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Mouse, Rat, Synthetic peptide samples.

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Images

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] - BSA and Azide free (AB320639), expandable thumbnail
  • Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] - BSA and Azide free (AB320639), expandable thumbnail
  • Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] - BSA and Azide free (AB320639), expandable thumbnail
  • Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] - BSA and Azide free (AB320639), expandable thumbnail
  • Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] - BSA and Azide free (AB320639), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotIHC-P
Human
Tested
Expected
Not recommended
Mouse
Tested
Expected
Not recommended
Rat
Tested
Expected
Not recommended
Synthetic peptide
Not recommended
Tested
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human, Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle (PubMed:16690701). In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise (PubMed:16690701). In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity). Phosphorylates reticulophagy regulator RETREG1 at 'Ser-151' under endoplasmic reticulum stress conditions which enhances RETREG1 oligomerization and its membrane scission and reticulophagy activity (PubMed:31930741).

Additional Targets

CAMK2A phospho T286, CAMK2D phospho T287, CAMK2G phospho T287

Alternative names

CAM2, CAMK2, CAMKB, CAMK2B, Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta

Recommended products

Rabbit Recombinant Monoclonal KCC2B phospho T287 antibody. Carrier free. Suitable for WB, Dot and reacts with Transfected cell lysate - Human, Human, Mouse, Rat, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28075-37
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab320639 is the carrier-free version of Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] ab320638.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The CaMKII (Ca2+/calmodulin-dependent protein kinase II) family consists of four isoforms: CaMKII alpha CaMKII beta CaMKII delta and CaMKII gamma also known respectively as CAMK2A CAMK2B CAMK2D and CAMK2G. These are serine/threonine-specific protein kinases with a molecular weight of around 50 to 60 kDa. They play a role in the phosphorylation of various downstream targets. These kinases are found in many tissues but CaMKII alpha is mostly found in brain tissue CaMKII beta in muscle tissue whereas CaMKII delta and gamma show more widespread expression across other tissues. The functional unit of CaMKII is a holoenzyme which forms a dodecamer consisting of 12 subunits.

Biological function summary

CaMKII isoforms participate in critical calcium signaling processes. They act to decode the frequency of calcium oscillations in cells and are involved in synaptic plasticity and memory formation. CaMKII alpha for example is highly active in the postsynaptic density where it can form complexes with both NMDA and AMPA receptors suggesting a role in the regulation of synaptic strength. While not part of a permanent complex CaMKII enzymes can transiently bind to other proteins during signaling events.

Pathways

CaMKII isoforms play significant roles in the calcium/calmodulin signal transduction pathway and the MAPK (mitogen-activated protein kinase) signaling cascade. In the calcium/calmodulin pathway CaMKII acts to mediate calcium-dependent phosphorylation events that affect neuronal and cardiac functions. It interacts with other pathway proteins such as calmodulin and CREB (cAMP response element-binding protein). In the MAPK pathway CaMKII modulates signals that control cell growth differentiation and response to cellular stress interacting with proteins like MEK and ERK.

Associated diseases and disorders

CaMKII isoforms have connections to neurodegenerative diseases such as Alzheimer's disease and to cardiac conditions like heart failure. In Alzheimer's disease altered CaMKII activity can affect synaptic function linking to proteins like amyloid-beta peptides. In cardiac disorders abnormal CaMKII activation contributes to disrupted calcium homeostasis and arrhythmia where it interacts with proteins such as RyR2 (ryanodine receptor 2). Understanding these interactions highlights CaMKII's significance in both brain and heart health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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