Rabbit Recombinant Monoclonal CaMKI antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859), expandable thumbnail](https://content.abcam.com/products/images/camki-antibody-epr2217y-bsa-and-azide-free-ab239859--western-blot-img165680.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859), expandable thumbnail](https://content.abcam.com/products/images/camki-antibody-epr2217y-bsa-and-azide-free-ab239859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img173078.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859), expandable thumbnail](https://content.abcam.com/products/images/camki-antibody-epr2217y-bsa-and-azide-free-ab239859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119566.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected |
| Mouse | Predicted | Not recommended | Not recommended | Expected |
| Rat | Predicted | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
Alternative names
Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaM-KI, CaMKI-alpha, CAMK1
Recommended products
Rabbit Recombinant Monoclonal CaMKI antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2217Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239859 is the carrier-free version of Anti-CaMKI antibody [EPR2217Y] ab68234.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CaMKI also known as Calcium/Calmodulin-dependent protein kinase I is a serine/threonine-specific protein kinase with an approximate molecular mass of 42 to 58 kDa depending on the isoform. It plays an important mechanical role in converting calcium signals to cellular responses through phosphorylation of various substrates. This protein is ubiquitously expressed across different tissues including brain muscle and liver where it participates in diverse cellular processes.
- Biological function summary
CaMKI functions as an essential mediator within the calcium/calmodulin-dependent protein kinase family. It modifies cellular activities by phosphorylating a range of downstream targets. Although CaMKI acts separately it associates with other family members like CaMKII sharing similar biochemical responses. In neurons it influences processes like synaptic plasticity and neurotransmitter release which are vital for learning and memory.
- Pathways
It interacts within the calcium-signaling pathway and the MAPK/ERK pathway. Through calcium signaling CaMKI modulates gene transcription and apoptosis often working alongside other kinases like CaMKIV. In the MAPK/ERK pathway it plays a role in regulating cellular proliferation and differentiation ensuring proper cellular responses to external stimuli.
- Associated diseases and disorders
Alterations in CaMKI activity have links to neurological and cardiovascular conditions. Dysregulation of CaMKI especially in its interaction with proteins such as synapsin I is connected to cognitive impairments like those seen in neurodegenerative diseases. Moreover its involvement in heart tissue relates it to cardiac hypertrophy where inappropriate signaling can impact heart function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859), expandable thumbnail](https://content.abcam.com/products/images/camki-antibody-epr2217y-bsa-and-azide-free-ab239859--western-blot-img165680.jpg "Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859)")
Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859)
Exposure time: Lane 1 & 3, 4, 5: 5 seconds. Lane 2 & 6：20 secondsBlocking and diluting buffer: 5% NFDM /TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CaMKI antibody [EPR2217Y] ab68234).
All lanes: Western blot - Anti-CaMKI antibody [EPR2217Y] (Anti-CaMKI antibody [EPR2217Y] ab68234) at 1/1000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 40 µg
Lane 2: Human brain tissue lysate at 40 µg
Lane 3: Mouse brain tissue lysate at 40 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast) at 40 µg
Lane 5: Rat brain tissue lysate at 40 µg
Lane 6: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859), expandable thumbnail](https://content.abcam.com/products/images/camki-antibody-epr2217y-bsa-and-azide-free-ab239859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img173078.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859)
Immunohistochemical analysis of Paraffin-embedded Human adrenal gland tissue labelling CaMKI with Anti-CaMKI antibody [EPR2217Y] ab68234 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the zona glomerulosa of human adrenal glands. The section was incubated with Anti-CaMKI antibody [EPR2217Y] ab68234 at +4°C overnight. Counter stained with Hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859), expandable thumbnail](https://content.abcam.com/products/images/camki-antibody-epr2217y-bsa-and-azide-free-ab239859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119566.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (ab239859)
Anti-CaMKI antibody [EPR2217Y] ab68234 at 1/100-1/250 dilution staining CaMKI in human kidney tissue sections.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CaMKI antibody [EPR2217Y] ab68234).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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