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AB239859

Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CaMKI antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CaM-KI, CaMKI-alpha, CAMK1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

Immunohistochemical analysis of Paraffin-embedded Human adrenal gland tissue labelling CaMKI with ab68234 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the zona glomerulosa of human adrenal glands. The section was incubated with ab68234 at +4°C overnight. Counter stained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

ab68234 at 1/100-1/250 dilution staining CaMKI in human kidney tissue sections.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68234).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)
  • WB

Lab

Western blot - Anti-CaMKI antibody [EPR2217Y] - BSA and Azide free (AB239859)

Exposure time : Lane 1 & 3, 4, 5 : 5 seconds. Lane 2 & 6：20 seconds

Blocking and diluting buffer : 5% NFDM /TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab68234).

All lanes:

Western blot - Anti-CaMKI antibody [EPR2217Y] (<a href='/en-us/products/primary-antibodies/camki-antibody-epr2217y-ab68234'>ab68234</a>) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 2:

Human brain tissue lysate at 40 µg

Lane 3:

Mouse brain tissue lysate at 40 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) at 40 µg

Lane 5:

Rat brain tissue lysate at 40 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2217Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB62215

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

ab239859 is the carrier-free version of ab68234.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKI also known as Calcium/Calmodulin-dependent protein kinase I is a serine/threonine-specific protein kinase with an approximate molecular mass of 42 to 58 kDa depending on the isoform. It plays an important mechanical role in converting calcium signals to cellular responses through phosphorylation of various substrates. This protein is ubiquitously expressed across different tissues including brain muscle and liver where it participates in diverse cellular processes.
Biological function summary

CaMKI functions as an essential mediator within the calcium/calmodulin-dependent protein kinase family. It modifies cellular activities by phosphorylating a range of downstream targets. Although CaMKI acts separately it associates with other family members like CaMKII sharing similar biochemical responses. In neurons it influences processes like synaptic plasticity and neurotransmitter release which are vital for learning and memory.

Pathways

It interacts within the calcium-signaling pathway and the MAPK/ERK pathway. Through calcium signaling CaMKI modulates gene transcription and apoptosis often working alongside other kinases like CaMKIV. In the MAPK/ERK pathway it plays a role in regulating cellular proliferation and differentiation ensuring proper cellular responses to external stimuli.

Alterations in CaMKI activity have links to neurological and cardiovascular conditions. Dysregulation of CaMKI especially in its interaction with proteins such as synapsin I is connected to cognitive impairments like those seen in neurodegenerative diseases. Moreover its involvement in heart tissue relates it to cardiac hypertrophy where inappropriate signaling can impact heart function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
See full target information CAMK1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International archives of allergy and immunology 182:1245-1254 PubMed34428765

2021

Celastrol Exerts Cardioprotective Effect in Rheumatoid Arthritis by Inhibiting TLR2/HMGB1 Signaling Pathway-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Lu,Sha Gong,Xiaojun Wang,Nan Hu,Dan Pu,Jing Zhang,Yanhua Wang,Jing Luo,Qi An,Bomiao Ju,Lan He
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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