Anti-CaMKII alpha antibody [6G9] (ab22609) is a mouse monoclonal antibody detecting CaMKII alpha in Western Blot, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.
- Over 90 publications
- Trusted since 2005
Images
![Immunohistochemistry (Frozen sections) - Anti-CaMKII alpha antibody [6G9] (AB22609), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-6g9-ab22609--immunohistochemistry-frozen-sections-img439635.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CaMKII alpha antibody [6G9] (AB22609)")
![Western blot - Anti-CaMKII alpha antibody [6G9] (AB22609), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-6g9-ab22609--western-blot-img93667.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CaMKII alpha antibody [6G9] (AB22609)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody [6G9] (AB22609), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-6g9-ab22609--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13521.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody [6G9] (AB22609)")
Publications
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- Cellular and molecular life sciences : CMLS 80:2892023Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunjian Dai,Taofeng Wei,Yuwen Huang,Yun Bei,Haoran Lin,Zexu Shen,Lingyan Yu,Mingdong Yang,Huimin Xu,Wei He,Zheng Lin,Haibin DaiPubMed 37690046
- eLife 12:2023Epigenetically distinct synaptic architecture in clonal compartments in the teleostean dorsal pallium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuko Isoe,Ryohei Nakamura,Shigenori Nonaka,Yasuhiro Kamei,Teruhiro Okuyama,Naoyuki Yamamoto,Hideaki Takeuchi,Hiroyuki TakedaPubMed 37489039
- Frontiers in molecular neuroscience 16:11511622023Glycolysis mediates neuron specific histone acetylation in valproic acid-induced human excitatory neuron differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndi Chen,Mengmeng Wang,Chao Xu,Youyi Zhao,Panpan Xian,Yuqian Li,Weian Zheng,Xuyang Yi,Shengxi Wu,Yazhou WangPubMed 37089691
- Science advances 9:eade53972023GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiromichi Inaba,Haiyan Li,Ayako Kawatake-Kuno,Ken-Ichi Dewa,Jun Nagai,Naoya Oishi,Toshiya Murai,Shusaku UchidaPubMed 37018397
- Science advances 9:eabo56272023Coordinated activity of a central pathway drives associative opioid analgesic tolerance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiwen Hou,Guichang Zou,Xianglian Wang,Hui Guo,Xiao Ma,Xingyu Cheng,Zhiyong Xie,Xin Zuo,Jing Xia,Huanhuan Mao,Man Yuan,Qi Chen,Peng Cao,Yupeng Yang,Li Zhang,Wei XiongPubMed 36753548
- iScience 26:1058302023Serotonergic neurons control cortical neuronal intracellular energy dynamics by modulating astrocyte-neuron lactate shuttle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkiyo Natsubori,Shinobu Hirai,Soojin Kwon,Daisuke Ono,Fei Deng,Jinxia Wan,Momoka Miyazawa,Takashi Kojima,Haruo Okado,Akihiro Karashima,Yulong Li,Kenji F Tanaka,Makoto HondaPubMed 36713262
- EMBO reports 23:e545432022eEF2 in the prefrontal cortex promotes excitatory synaptic transmission and social novelty behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuanyue Ma,Liuren Li,Ziming Li,Zhengyi Huang,Yaorong Yang,Peng Liu,Daji Guo,Yueyao Li,Tianying Wu,Ruixiang Luo,Junyu Xu,Wen-Cai Ye,Bin Jiang,Lei ShiPubMed 35993189
- Journal of thoracic disease 14:2187-22002022Moricizine prevents atrial fibrillation by late sodium current inhibition in atrial myocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Zou,Qingxing Chen,Chaofeng Chen,Guijian Liu,Yunlong Ling,Yang Pang,Ye Xu,Kuan Cheng,Wenqing Zhu,Ru-Xing Wang,Ling-Ling Qian,Junbo GePubMed 35813708
- Cell reports 39:1108822022A cholinergic medial septum input to medial habenula mediates generalization formation and extinction of visual aversion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRonghao Mu,Susu Tang,Xiaomeng Han,Hao Wang,Danhua Yuan,Jiajia Zhao,Yan Long,Hao HongPubMed 35649349
- Signal transduction and targeted therapy 7:1702022BOD1 regulates the cerebellar IV/V lobe-fastigial nucleus circuit associated with motor coordination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiu-Xiu Liu,Xing-Hui Chen,Zhi-Wei Zheng,Qin Jiang,Chen Li,Lin Yang,Xiang Chen,Xing-Feng Mao,Hao-Yang Yuan,Li-Li Feng,Quan Jiang,Wei-Xing Shi,Takuya Sasaki,Kohji Fukunaga,Zhong Chen,Feng Han,Ying-Mei LuPubMed 35641478
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Rat | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Version
7 products for Alternative Product
Target data
Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed:14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed:11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed:35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
Alternative names
CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha
Recommended products
Anti-CaMKII alpha antibody [6G9] (ab22609) is a mouse monoclonal antibody detecting CaMKII alpha in Western Blot, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.
- Over 90 publications
- Trusted since 2005
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 6G9
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CaMKII alpha antibody [6G9] (ab22609) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
What is the molecular weight of CaMKII alpha?
Anti-CaMKII alpha [6G9] (ab22609) specifically detects a band for CaMKII alpha (UniProt: Q9UQM7) at a molecular weight of 56kDa.
Trusted by the scientific community
Anti-CaMKII alpha [6G9] (ab22609) was first used in a scientific publication in 2005 and has been cited over 90 times in peer-reviewed journals.
Reviewed by scientists
Anti-CaMKII alpha [6G9] (ab22609) has over 5 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [6G9] also available for your convenience:
ab22609, Carrier free - Anti-CaMKII alpha antibody [6G9] - BSA and Azide free ab187488, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-CaMKII alpha antibody [6G9] ab197497, HRP - HRP Anti-CaMKII alpha antibody [6G9] ab197500
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.
- Biological function summary
CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.
- Pathways
CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.
- Associated diseases and disorders
Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
![Immunohistochemistry (Frozen sections) - Anti-CaMKII alpha antibody [6G9] (ab22609), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-6g9-ab22609--immunohistochemistry-frozen-sections-img439635.jpg "Immunohistochemistry (Frozen sections) - Anti-CaMKII alpha antibody [6G9] (ab22609)")
Immunohistochemistry (Frozen sections) - Anti-CaMKII alpha antibody [6G9] (ab22609)
IHC image of CaMKII alpha staining in a section of frozen normal rat brain performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab22609, 1 µg/mL, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Western blot - Anti-CaMKII alpha antibody [6G9] (ab22609), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-6g9-ab22609--western-blot-img93667.jpg "Western blot - Anti-CaMKII alpha antibody [6G9] (ab22609)")
Western blot - Anti-CaMKII alpha antibody [6G9] (ab22609)
All lanes: Western blot - Anti-CaMKII alpha antibody [6G9] (ab22609) at 10 µg/mL
Lane 1: Brain (Rat) Tissue Lysate at 20 µg
Lane 2: Brain (Mouse) Tissue Lysate at 20 µg
Lane 3: Brain (Human) Tissue Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 100 kDa, 50 kDa, 65 kDa, 70 kDa
Exposure time: 5min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody [6G9] (ab22609), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-6g9-ab22609--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13521.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody [6G9] (ab22609)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody [6G9] (ab22609)
IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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