Anti-CaMKII alpha antibody [6G9] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody [6G9] - BSA and Azide free (AB187488)

This data was developed using ab22609, the same antibody clone in a different buffer formulation.

IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CaMKII alpha antibody [6G9] - BSA and Azide free (AB187488)

This data was developed using ab22609, the same antibody clone in a different buffer formulation.

IHC image of CaMKII alpha staining in a section of frozen normal rat brain performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab22609, 1 µg/mL, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Lab

Western blot - Anti-CaMKII alpha antibody [6G9] - BSA and Azide free (AB187488)

This data was developed using ab22609, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CaMKII alpha antibody [6G9] (<a href='/en-us/products/primary-antibodies/camkii-alpha-antibody-6g9-ab22609'>ab22609</a>) at 10 µg/mL

Lane 1:

Brain (Rat) Tissue Lysate at 20 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 20 µg

Lane 3:

Brain (Human) Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 54 kDa

Observed band size: 100 kDa,50 kDa,65 kDa,70 kDa

false

Exposure time: 5min