AB103840

Anti-CaMKII alpha antibody

BOND RX™ Validated
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(5 Publications)

Rabbit Polyclonal KCC2A antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples. Cited in 5 publications.

View Alternative Names

CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody (AB103840)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody (AB103840)

IHC image of CaMKII alpha staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103840, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-CaMKII alpha antibody (AB103840)

All lanes:

Western blot - Anti-CaMKII alpha antibody (ab103840) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Brain (Rat) Tissue Lysate at 10 µg

Lane 3:

Mouse cortex Mouse Tissue Lysate at 10 µg

Lane 4:

Rat Cortex Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 54 kDa

Observed band size: 50 kDa,54 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.

Biological function summary

CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.

Pathways

CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.

Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of PSAT1, inhibiting ferroptosis by promoting GPX4 hydroxylation and stability (PubMed : 40281343). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

See full target information CAMK2A

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70160 PubMed39817595

2025

CHOP-Mediated Disruption of Hippocampal Synaptic Plasticity and Neuronal Activity Contributes to Chronic Pain-Related Cognitive Deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsheng Meng,Songxue Su,Lei Lei,Yubing Zhang,Jiabin Duan,Xiuhua Ren,Yihang Song,Xiaoyu Hu,Shiyue Chen,Weidong Zang,Zhen Zhang,Jing Cao

BMC biology 21:113 PubMed37221592

2023

CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Woelfle,Dhruva Deshpande,Simone Feldengut,Heiko Braak,Kelly Del Tredici,Francesco Roselli,Karl Deisseroth,Jens Michaelis,Tobias M Boeckers,Michael Schön

Cell reports 35:108985 PubMed33852843

2021

Dietary spermidine improves cognitive function.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Schroeder,Sebastian J Hofer,Andreas Zimmermann,Raimund Pechlaner,Christopher Dammbrueck,Tobias Pendl,G Mark Marcello,Viktoria Pogatschnigg,Martina Bergmann,Melanie Müller,Verena Gschiel,Selena Ristic,Jelena Tadic,Keiko Iwata,Gesa Richter,Aitak Farzi,Muammer Üçal,Ute Schäfer,Michael Poglitsch,Philipp Royer,Ronald Mekis,Marlene Agreiter,Regine C Tölle,Péter Sótonyi,Johann Willeit,Barbara Mairhofer,Helga Niederkofler,Irmgard Pallhuber,Gregorio Rungger,Herbert Tilg,Michaela Defrancesco,Josef Marksteiner,Frank Sinner,Christoph Magnes,Thomas R Pieber,Peter Holzer,Guido Kroemer,Didac Carmona-Gutierrez,Luca Scorrano,Jörn Dengjel,Tobias Madl,Simon Sedej,Stephan J Sigrist,Bence Rácz,Stefan Kiechl,Tobias Eisenberg,Frank Madeo

Nature communications 11:2755 PubMed32488011

2020

FMRP(1-297)-tat restores ion channel and synaptic function in a model of Fragile X syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqin Zhan,Hadhimulya Asmara,Ning Cheng,Giriraj Sahu,Eduardo Sanchez,Fang-Xiong Zhang,Gerald W Zamponi,Jong M Rho,Ray W Turner

Physiological reports 7:e13972 PubMed30806037

2019

Exercise training reduces ventricular arrhythmias through restoring calcium handling and sympathetic tone in myocardial infarction mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rujie Qin,Nobuyuki Murakoshi,DongZhu Xu,Kazuko Tajiri,Duo Feng,Endin N Stujanna,Saori Yonebayashi,Yoshimi Nakagawa,Hitoshi Shimano,Akihiko Nogami,Akira Koike,Kazutaka Aonuma,Masaki Ieda

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CaMKII alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody (AB103840)

Western blot - Anti-CaMKII alpha antibody (AB103840)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise