AB111890

Anti-CaMKII alpha antibody

Name: Anti-CaMKII alpha antibody (ab111890) | Abcam
Brand: Abcam Limited
SKU: AB111890

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(2 Publications)

Goat Polyclonal KCC2A antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CAMK2A.

View Alternative Names

CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody (AB111890)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody (AB111890)

ab111890, at 3.75 µg/ml, staining CaMKII alpha in formalin fixed, paraffin embedded Human brain cortex by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody (AB111890)

ab111890, at 3.75 µg/ml, staining CaMKII alpha in formalin fixed, paraffin embedded Human adrenal by Immunohistochemistry.

Unknown

Western blot - Anti-CaMKII alpha antibody (AB111890)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-CaMKII alpha antibody (ab111890) at 0.1 µg/mL

All lanes:

Mouse brain lysate in RIPA buffer at 35 µg

Predicted band size: 54 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CAMK2A. The exact immunogen used to generate this antibody is proprietary information.

Q9UQM7

Specificity

ab111890 is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1). This antibody does cross-react with CAMKII beta protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store undiluted

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.

Biological function summary

CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.

Pathways

CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.

Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

See full target information CAMK2A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 4:138 PubMed33514783

2021

Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Takaaki Furihata,Shingo Takada,Naoya Kakutani,Satoshi Maekawa,Masaya Tsuda,Junichi Matsumoto,Wataru Mizushima,Arata Fukushima,Takashi Yokota,Nobuyuki Enzan,Shouji Matsushima,Haruka Handa,Yoshizuki Fumoto,Junko Nio-Kobayashi,Toshihiko Iwanaga,Shinya Tanaka,Hiroyuki Tsutsui,Hisataka Sabe,Shintaro Kinugawa

Frontiers in behavioral neuroscience 13:227 PubMed31636548

2019

Glutamate Afferents From the Medial Prefrontal Cortex Mediate Nucleus Accumbens Activation by Female Sexual Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsey M Moore,Wyatt L Oelberg,M Rose Glass,Matthew D Johnson,Laura E Been,Robert L Meisel

View all publications

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