Goat Polyclonal KCC2A antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CAMK2A.
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- Frontiers in behavioral neuroscience 13:2272019Glutamate Afferents From the Medial Prefrontal Cortex Mediate Nucleus Accumbens Activation by Female Sexual Behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelsey M Moore et. al.PubMed 31636548
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CAMK2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQM7
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Cow, Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.1-0.3 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Cow, Dog | Dilution info - | Notes - |
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Target data
Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed:14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed:11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed:35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
Alternative names
CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha
Recommended products
Goat Polyclonal KCC2A antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CAMK2A.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CAMK2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQM7
- Purification technique
- Affinity purification Immunogen
- Specificity
ab111890 is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
This antibody does cross-react with CAMKII beta protein- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Store undiluted
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.
- Biological function summary
CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.
- Pathways
CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.
- Associated diseases and disorders
Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-CaMKII alpha antibody (ab111890)
Primary incubation was 1 hour. Detected by chemiluminescence.
All lanes: Western blot - Anti-CaMKII alpha antibody (ab111890) at 0.1 µg/mL
All lanes: Mouse brain lysate in RIPA buffer at 35 µg
Predicted band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody (ab111890)
ab111890, at 3.75 µg/ml, staining CaMKII alpha in formalin fixed, paraffin embedded Human adrenal by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII alpha antibody (ab111890)
ab111890, at 3.75 µg/ml, staining CaMKII alpha in formalin fixed, paraffin embedded Human brain cortex by Immunohistochemistry.
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