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AB131468

Anti-CaMKII antibody

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(9 Publications)

Rabbit Polyclonal KCC2A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human CAMK2A.

View Alternative Names

CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody (AB131468)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody (AB131468)

Immunofluorescence staining of Hela cells, using CaMKII (ab131468) antibody at 1/100 dilution.

Western blot - Anti-CaMKII antibody (AB131468)
  • WB

Supplier Data

Western blot - Anti-CaMKII antibody (AB131468)

All lanes:

Western blot - Anti-CaMKII antibody (ab131468) at 1/1000 dilution

Lane 1:

Hela whole cell lysates at 20 µg

Lane 2:

A431 (epidermoid carcinoma cell line) whole cell lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

Rat brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Observed band size: 60 kDa,45 kDa

false

Exposure time: 5s

Western blot - Anti-CaMKII antibody (AB131468)
  • WB

Unknown

Western blot - Anti-CaMKII antibody (AB131468)

All lanes:

Western blot - Anti-CaMKII antibody (ab131468) at 1/500 dilution

Lane 1:

Extracts from mouse brain tissue

Lane 2:

Extracts from mouse brain tissue with blocking peptide

Predicted band size: 10 kDa,45 kDa,49 kDa,54 kDa,56 kDa,61 kDa,66 kDa,75 kDa

Observed band size: 35 kDa,49 kDa,50 kDa,70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CAMK2A. The exact immunogen used to generate this antibody is proprietary information.

Q9UQM7

Specificity

This antibody does cross-react with CAMKII beta protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab131468 was purified by affinity-chromatography using epitope-specific peptide.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.
Biological function summary

CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.

Pathways

CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.

Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
See full target information CAMK2A
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Neurobiology of stress 33:100680 PubMed39502835

2024

Cholecystokinin-expressing interneurons mediated inhibitory transmission and plasticity in basolateral amygdala modulate stress-induced anxiety-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Fang,Xi Chen,Jufang He

CNS neuroscience & therapeutics 30:e14764 PubMed38828629

2024

SIRT1 mediates the excitability of spinal CaMKIIα-positive neurons and participates in neuropathic pain by controlling Nav1.3.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanzeng Wang,Yidan Zhang,Nan Ma,Wen Zhao,Xiuhua Ren,Yanyan Sun,Weidong Zang,Jing Cao

Nature communications 14:7476 PubMed37978295

2023

GDF11 slows excitatory neuronal senescence and brain ageing by repressing p21.

Applications

Unspecified application

Species

Unspecified reactive species

Di-Xian Wang,Zhao-Jun Dong,Sui-Xin Deng,Ying-Ming Tian,Yu-Jie Xiao,Xinran Li,Xiao-Ru Ma,Liang Li,Pengxiao Li,Hui-Zhong Chang,Longqi Liu,Fan Wang,Yang Wu,Xiang Gao,Shuang-Shuang Zheng,Hui-Min Gu,Ya-Nan Zhang,Jian-Bin Wu,Fan Wu,Yonglin Peng,Xiao-Wen Zhang,Ren-Ya Zhan,Li-Xia Gao,Qiming Sun,Xing Guo,Xiao-Dong Zhao,Jian-Hong Luo,Ruhong Zhou,Lei Han,Yousheng Shu,Jing-Wei Zhao

Hippocampus 33:730-744 PubMed36971428

2023

Mechanisms of mGluR-dependent plasticity in hippocampal area CA2.

Applications

Unspecified application

Species

Unspecified reactive species

Mahsa Samadi,Claire A Hales,Daniel J Lustberg,Shannon Farris,Madeleine R Ross,Meilan Zhao,John R Hepler,Nicholas H Harbin,Emma S J Robinson,Paul J Banks,Zafar I Bashir,Serena M Dudek

Frontiers in cellular neuroscience 16:821885 PubMed35250487

2022

Mild Traumatic Brain Injury-Induced Disruption of the Blood-Brain Barrier Triggers an Atypical Neuronal Response.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Munoz-Ballester,Dzenis Mahmutovic,Yusuf Rafiqzad,Alia Korot,Stefanie Robel

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 41:530-545 PubMed32248729

2020

Rbfox-1 contributes to CaMKIIα expression and intracerebral hemorrhage-induced secondary brain injury via blocking micro-RNA-124.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Shen,Xiang Xu,Zhengquan Yu,Haiying Li,Haitao Shen,Xiang Li,Meifen Shen,Gang Chen

eLife 7: PubMed30387713

2018

CA2 neuronal activity controls hippocampal low gamma and ripple oscillations.

Applications

Unspecified application

Species

Unspecified reactive species

Georgia M Alexander,Logan Y Brown,Shannon Farris,Daniel Lustberg,Caroline Pantazis,Bernd Gloss,Nicholas W Plummer,Patricia Jensen,Serena M Dudek

Journal of radiation research 58:809-815 PubMed29106600

2017

Chronic irradiation with low-dose-rate 137Cs-γ rays inhibits NGF-induced neurite extension of PC12 cells via Ca2+/calmodulin-dependent kinase II activation.

Applications

Unspecified application

Species

Unspecified reactive species

Katoh Shinsuke,Kobayashi Junya,Umeda Tomonobu,Kobayashi Yoshiko,Nobuo Izumo,Suzuki Takahiko

PloS one 12:e0178098 PubMed28542400

2017

Intracellular Ca2+ homeostasis and JAK1/STAT3 pathway are involved in the protective effect of propofol on BV2 microglia against hypoxia-induced inflammation and apoptosis.

Applications

WB

Species

Unspecified reactive species

Yan Lu,Yuechao Gu,Xiaowei Ding,Jiaqiang Wang,Jiawei Chen,Changhong Miao
View all publications
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