AB92332

Anti-CaMKII alpha antibody [EPR1828]

RabMAb
Recombinant
What is this?

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(23 Publications)

Rabbit Recombinant Monoclonal KCC2A antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human, Mouse samples. Cited in 23 publications.

View Alternative Names

CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha

2 Images

Flow Cytometry (Intracellular) - Anti-CaMKII alpha antibody [EPR1828] (AB92332)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CaMKII alpha antibody [EPR1828] (AB92332)

Overlay histogram showing SH-SY5Y cells stained with ab92332 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92332, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Western blot - Anti-CaMKII alpha antibody [EPR1828] (AB92332)

All lanes:

Western blot - Anti-CaMKII alpha antibody [EPR1828] (ab92332)

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

SH SY5Y cell lysate at 10 µg

Lane 3:

Neuro 2a cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 54 kDa

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Carrier free

Anti-CaMKII alpha antibody [EPR1828] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1828

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The peptide immunogen shares a 100% homology with CaMKII beta, gamma and delta therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.

Biological function summary

CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.

Pathways

CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.

Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of PSAT1, inhibiting ferroptosis by promoting GPX4 hydroxylation and stability (PubMed : 40281343). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

See full target information CAMK2A

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 24:e14349 PubMed39380368

2024

CaMKIIα-TARPγ8 signaling mediates hippocampal synaptic impairment in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao JianHua,MingCan Li,Qilin Hu,Peter Donoghue,Sanwei Jiang,Junmei Li,Songji Li,Xinyi Ren,Ziyuan Zhang,Jingzhi Du,Yi Yu,Paul Chazot,Chengbiao Lu

Cell death & disease 15:429 PubMed38890273

2024

Tau disengages from the proteosome core complex and neurogranin coincident with enhanced neuronal network excitability.

Applications

Unspecified application

Species

Unspecified reactive species

Katriona L Hole,Bangfu Zhu,Laura Huggon,Jon T Brown,Jody M Mason,Robert J Williams

Oncology reports 51: PubMed38577936

2024

Butyrate increases methylglyoxal production through regulation of the JAK2/Stat3/Nrf2/Glo1 pathway in castration‑resistant prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Jan Hsia,Zhang-Min Lin,Taolan Zhang,Tz-Chong Chou

iScience 26:108306 PubMed38026209

2023

Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Zheng,Zhixian Liu,Ziyu He,Jia Xu,YaNan Wang,ChenZi Gong,Ruoying Zhang,Su-Chun Zhang,Hong Chen,Wei Wang

European journal of pain (London, England) 28:297-309 PubMed37668323

2023

Effect of spirocyclopiperazinium salt compound LXM-15 on spinal nerve injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Yu Wang,Ying Ying Liang,Qin Liu,Ding Wang,Qi Sun,Run Tao Li,Hua Yang,Yi Min Jiang,Jia Ye

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:2631-2652 PubMed36868861

2023

Chronic Neuronal Inactivity Utilizes the mTOR-TFEB Pathway to Drive Transcription-Dependent Autophagy for Homeostatic Up-Scaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Jingran Lin,Jiarui Li,Lu Yan,Wenwen Li,Xingzhi He,Huan Ma

Glia 70:2392-2408 PubMed35946355

2022

Astrocyte-specific loss of lactoferrin influences neuronal structure and function by interfering with cholesterol synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang-Feng Xu,Zhong-Qiu Pang,Yong-Gang Fan,Yan-Hui Zhang,Yu-Han Meng,Chen-Yang Bai,Meng-Yu Jia,Yan-Hong Chen,Zhan-You Wang,Chuang Guo

Molecular neurobiology 59:1649-1664 PubMed35001354

2022

Soybean Meal Extract Preserves Memory Ability by Increasing Presynaptic Function and Modulating Gut Microbiota in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Kun-Chieh Yeh,Chi-Feng Hung,Hui-Ling Lee,Ting-Yang Hsieh,Su-Jane Wang

Journal of neuroinflammation 18:303 PubMed34952606

2021

Interplay between Müller cells and microglia aggravates retinal inflammatory response in experimental glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Hu,Guo-Li Zhao,Meng-Xi Xu,Han Zhou,Fang Li,Yanying Miao,Bo Lei,Xiong-Li Yang,Zhongfeng Wang

EMBO molecular medicine 13:e12574 PubMed33656268

2021

Cooperation of LIM domain-binding 2 (LDB2) with EGR in the pathogenesis of schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuo Ohnishi,Yuji Kiyama,Fumiko Arima-Yoshida,Mitsutaka Kadota,Tomoe Ichikawa,Kazuyuki Yamada,Akiko Watanabe,Hisako Ohba,Kaori Tanaka,Akihiro Nakaya,Yasue Horiuchi,Yoshimi Iwayama,Manabu Toyoshima,Itone Ogawa,Chie Shimamoto-Mitsuyama,Motoko Maekawa,Shabeesh Balan,Makoto Arai,Mitsuhiro Miyashita,Kazuya Toriumi,Yayoi Nozaki,Rumi Kurokawa,Kazuhiro Suzuki,Akane Yoshikawa,Tomoko Toyota,Toshihiko Hosoya,Hiroyuki Okuno,Haruhiko Bito,Masanari Itokawa,Shigehiro Kuraku,Toshiya Manabe,Takeo Yoshikawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CaMKII alpha antibody [EPR1828]

Flow Cytometry (Intracellular) - Anti-CaMKII alpha antibody [EPR1828] (AB92332)

Western blot - Anti-CaMKII alpha antibody [EPR1828] (AB92332)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise