Rabbit Recombinant Monoclonal KCC2A antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human, Mouse samples. Cited in 18 publications.
Images
![Flow Cytometry (Intracellular) - Anti-CaMKII alpha antibody [EPR1828] (AB92332), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-epr1828-ab92332--flow-cytometry-intracellular-img88973.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CaMKII alpha antibody [EPR1828] (AB92332)")
![Western blot - Anti-CaMKII alpha antibody [EPR1828] (AB92332), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-epr1828-ab92332--western-blot-img15803.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CaMKII alpha antibody [EPR1828] (AB92332)")
Publications
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- iScience 26:1083062023Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolong Zheng,Zhixian Liu,Ziyu He,Jia Xu,YaNan Wang,ChenZi Gong,Ruoying Zhang,Su-Chun Zhang,Hong Chen,Wei WangPubMed 38026209
- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:2631-26522023Chronic Neuronal Inactivity Utilizes the mTOR-TFEB Pathway to Drive Transcription-Dependent Autophagy for Homeostatic Up-Scaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Wang,Jingran Lin,Jiarui Li,Lu Yan,Wenwen Li,Xingzhi He,Huan MaPubMed 36868861
- Glia 70:2392-24082022Astrocyte-specific loss of lactoferrin influences neuronal structure and function by interfering with cholesterol synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang-Feng Xu,Zhong-Qiu Pang,Yong-Gang Fan,Yan-Hui Zhang,Yu-Han Meng,Chen-Yang Bai,Meng-Yu Jia,Yan-Hong Chen,Zhan-You Wang,Chuang GuoPubMed 35946355
- Molecular neurobiology 59:1649-16642022Soybean Meal Extract Preserves Memory Ability by Increasing Presynaptic Function and Modulating Gut Microbiota in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun-Chieh Yeh,Chi-Feng Hung,Hui-Ling Lee,Ting-Yang Hsieh,Su-Jane WangPubMed 35001354
- Journal of neuroinflammation 18:3032021Interplay between Müller cells and microglia aggravates retinal inflammatory response in experimental glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Hu,Guo-Li Zhao,Meng-Xi Xu,Han Zhou,Fang Li,Yanying Miao,Bo Lei,Xiong-Li Yang,Zhongfeng WangPubMed 34952606
- EMBO molecular medicine 13:e125742021Cooperation of LIM domain-binding 2 (LDB2) with EGR in the pathogenesis of schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTetsuo Ohnishi,Yuji Kiyama,Fumiko Arima-Yoshida,Mitsutaka Kadota,Tomoe Ichikawa,Kazuyuki Yamada,Akiko Watanabe,Hisako Ohba,Kaori Tanaka,Akihiro Nakaya,Yasue Horiuchi,Yoshimi Iwayama,Manabu Toyoshima,Itone Ogawa,Chie Shimamoto-Mitsuyama,Motoko Maekawa,Shabeesh Balan,Makoto Arai,Mitsuhiro Miyashita,Kazuya Toriumi,Yayoi Nozaki,Rumi Kurokawa,Kazuhiro Suzuki,Akane Yoshikawa,Tomoko Toyota,Toshihiko Hosoya,Hiroyuki Okuno,Haruhiko Bito,Masanari Itokawa,Shigehiro Kuraku,Toshiya Manabe,Takeo YoshikawaPubMed 33656268
- Cells 10:2021Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElaheh Soleimanpour et. al.PubMed 33530478
- Nature communications 10:4862019ATM phosphorylation of the actin-binding protein drebrin controls oxidation stress-resistance in mammalian neurons and C. elegans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatricia Kreis et. al.PubMed 30700723
- Neurobiology of disease 117:149-1602018Pharmaco-genetic therapeutics targeting parvalbumin neurons attenuate temporal lobe epilepsy.
- Molecules and cells 41:486-4942018Impaired Hippocampal Synaptic Plasticity and Enhanced Excitatory Transmission in a Novel Animal Model of Autism Spectrum Disorders with Telomerase Reverse Transcriptase Overexpression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeehae Rhee et. al.PubMed 29696935
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed:14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed:11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed:35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
Alternative names
CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha
Recommended products
Rabbit Recombinant Monoclonal KCC2A antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human, Mouse samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR1828
- Purification technique
- Affinity purification Protein A
- Specificity
The peptide immunogen shares a 100% homology with CaMKII beta, gamma and delta therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.
- Biological function summary
CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.
- Pathways
CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.
- Associated diseases and disorders
Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-CaMKII alpha antibody [EPR1828] (ab92332), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-epr1828-ab92332--flow-cytometry-intracellular-img88973.jpg "Flow Cytometry (Intracellular) - Anti-CaMKII alpha antibody [EPR1828] (ab92332)")
Flow Cytometry (Intracellular) - Anti-CaMKII alpha antibody [EPR1828] (ab92332)
Overlay histogram showing SH-SY5Y cells stained with ab92332 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92332, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Western blot - Anti-CaMKII alpha antibody [EPR1828] (ab92332), expandable thumbnail](https://content.abcam.com/products/images/camkii-alpha-antibody-epr1828-ab92332--western-blot-img15803.jpg "Western blot - Anti-CaMKII alpha antibody [EPR1828] (ab92332)")
Western blot - Anti-CaMKII alpha antibody [EPR1828] (ab92332)
All lanes: Western blot - Anti-CaMKII alpha antibody [EPR1828] (ab92332)
Lane 1: Rat brain lysate at 10 µg
Lane 2: SH SY5Y cell lysate at 10 µg
Lane 3: Neuro 2a cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
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