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Anti-CaMKII antibody [EP1829Y] ab52476 is a rabbit monoclonal antibody that is used in CaMKII western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1829Y has been tried and trusted by researchers since 2007 and is cited in >100 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [EP1829Y] (AB52476), expandable thumbnail
  • Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476), expandable thumbnail
  • Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (AB52476), expandable thumbnail
  • Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Not recommended
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Tested
Expected
Expected
Rat
Not recommended
Not recommended
Tested
Tested
Expected

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

For unpurified use at 1/20000.

Species

Rat

Dilution info

1/1000 - 1/5000

Notes

For unpurified use at 1/20000.

Species

Human

Dilution info

1/1000 - 1/5000

Notes

For unpurified use at 1/20000.

Tested
Tested

Species

Rat

Dilution info

1/250

Notes

-

Species

Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

14 products for Alternative Product

1 product for Alternative Version

Target data

Function

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

Alternative names

Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, KIAA0968, CAMKA, CAMK2A

Recommended products

Anti-CaMKII antibody [EP1829Y] ab52476 is a rabbit monoclonal antibody that is used in CaMKII western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1829Y has been tried and trusted by researchers since 2007 and is cited in >100 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1829Y

Purification technique

Affinity purification Protein A

Specificity

The peptide immunogen is highly conserved between CaMKII alpha, beta, gamma, and delta.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CaMKII also known as calcium/calmodulin-dependent protein kinase II is an enzyme functioning critically in cellular signaling. It consists of multiple isoforms including alpha beta gamma and delta with the delta isoform Delta 1829 frequently highlighted for its specific roles. The molecular weight of CaMKII is approximately 50 to 60 kDa varying slightly between isoforms. CaMKII is widely expressed in the brain but is also present in the heart skeletal muscle and other tissues. Its structure includes a catalytic domain regulatory segment and association domain which allows auto-regulation and interaction with other molecules.

Biological function summary

CaMKII plays a vital role in cellular processes such as learning memory and muscle contraction. It can form a complex structure involving multiple subunits which allows for its unique ability to signal in response to calcium influx and calmodulin binding. This causes autophosphorylation of CaMKII allowing it to remain active even when calcium levels diminish. This property makes CaMKII essential for long-term potentiation (LTP) in neuron synapses influencing cognitive functions deeply.

Pathways

CaMKII is an important component of both the calcium signaling and synaptic plasticity pathways. In the calcium signaling pathway CaMKII interacts with calmodulin and other kinases to modulate calcium entry and signaling. In synaptic plasticity CaMKII works closely with proteins like NMDA receptors which regulate synaptic strength and modulation essential for encoding learning and memory. These interactions highlight its significance as a mediator between signaling cascades and cellular responses.

Associated diseases and disorders

Abnormal regulation or expression of CaMKII links to Alzheimer's disease and cardiac arrhythmias. In Alzheimer's the dysregulation of CaMKII affects synaptic integrity and memory formation involving interaction with beta-amyloid plaques. In cardiac arrhythmias CaMKII dysfunction leads to improper cardiac muscle contractions often linked to the protein phospholamban. These associations highlight the clinical relevance of CaMKII in addressing and understanding mechanisms underpinning these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [EP1829Y] (ab52476), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [EP1829Y] (ab52476)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CaMKII with purified ab52476 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476), expandable thumbnail

    Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/5000 dilution

    All lanes: Human fetal brain tissue lysate at 20 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa

    Observed band size: 45 kDa, 70 kDa

  • Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476), expandable thumbnail

    Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa

    Observed band size: 45 kDa, 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (ab52476), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (ab52476)

    Immunocytochemistry/Immunofluorescence analysis of U87-MG cells labelling CaMKII with purified ab52476 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% aTriton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476), expandable thumbnail

    Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476)

    All lanes: Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/20000 dilution

    All lanes: Mouse brain lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 54 kDa

    Observed band size: 45 kDa, 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (ab52476), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (ab52476)

    ICC/IF image of unpurified ab52476 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52476, 1μg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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