AB52476

Anti-CaMKII antibody [EP1829Y]

Name: Anti-CaMKII antibody [EP1829Y] (ab52476) | Abcam
Brand: Abcam Limited
SKU: AB52476
Price: 245 USD
Availability: InStock
Rating: 4 (7 reviews)

RabMAb
Recombinant
What is this?

(7 Reviews)

(154 Publications)

Anti-CaMKII antibody [EP1829Y] (ab52476) is a rabbit monoclonal antibody detecting CaMKII in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 100 publications
- Trusted since 2007

View Alternative Names

CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (AB52476)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (AB52476)

Immunocytochemistry/Immunofluorescence analysis of U87-MG cells labelling CaMKII with purified ab52476 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% aTriton X-100. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control : primary antibody (1/250) and secondary antibody ab150113 an Alexa Fluor^® 488-conjugated goat anti-mouse IgG (1/500).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [EP1829Y] (AB52476)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [EP1829Y] (AB52476)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CaMKII with purified ab52476 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (AB52476)

ICC/IF image of unpurified ab52476 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52476 1μg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Lab

Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/5000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 45 kDa,70 kDa

false

Lab

Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 45 kDa,70 kDa

false

Unknown

Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476)

All lanes:

Western blot - Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/20000 dilution

All lanes:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 54 kDa

Observed band size: 45 kDa,70 kDa

false

Carrier free

Anti-CaMKII antibody [EP1829Y] - BSA and Azide free
Carrier free

Anti-CaMKII antibody [EP1829Y] - Low endotoxin, Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CaMKII antibody [EP1829Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CaMKII antibody [EP1829Y]
HRP

HRP Anti-CaMKII antibody [EP1829Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1829Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The peptide immunogen is highly conserved between CaMKII alpha, beta, gamma, and delta.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/80", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "For unpurified use at 1/20000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/80", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "For unpurified use at 1/20000.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/80", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "For unpurified use at 1/20000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-CaMKII antibody [EP1829Y] (ab52476) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of CaMKII?
Anti-CaMKII [EP1829Y] (ab52476) specifically detects a band for CaMKII (UniProt: Q13554) at a molecular weight of 54kDa.

Trusted by the scientific community
Anti-CaMKII [EP1829Y] (ab52476) was first used in a scientific publication in 2007 and has been cited over 100 times in peer-reviewed journals.

Reviewed by scientists
Anti-CaMKII [EP1829Y] (ab52476) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP1829Y] also available for your convenience: ab52476, HRP - ab195518, Alexa Fluor® 647 - ab196165, Alexa Fluor® 488 - ab196378, Alexa Fluor® 555 - ab206883, Carrier free - ab219365, Carrier free - ab271841

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII also known as calcium/calmodulin-dependent protein kinase II is an enzyme functioning critically in cellular signaling. It consists of multiple isoforms including alpha beta gamma and delta with the delta isoform Delta 1829 frequently highlighted for its specific roles. The molecular weight of CaMKII is approximately 50 to 60 kDa varying slightly between isoforms. CaMKII is widely expressed in the brain but is also present in the heart skeletal muscle and other tissues. Its structure includes a catalytic domain regulatory segment and association domain which allows auto-regulation and interaction with other molecules.

Biological function summary

CaMKII plays a vital role in cellular processes such as learning memory and muscle contraction. It can form a complex structure involving multiple subunits which allows for its unique ability to signal in response to calcium influx and calmodulin binding. This causes autophosphorylation of CaMKII allowing it to remain active even when calcium levels diminish. This property makes CaMKII essential for long-term potentiation (LTP) in neuron synapses influencing cognitive functions deeply.

Pathways

CaMKII is an important component of both the calcium signaling and synaptic plasticity pathways. In the calcium signaling pathway CaMKII interacts with calmodulin and other kinases to modulate calcium entry and signaling. In synaptic plasticity CaMKII works closely with proteins like NMDA receptors which regulate synaptic strength and modulation essential for encoding learning and memory. These interactions highlight its significance as a mediator between signaling cascades and cellular responses.

Abnormal regulation or expression of CaMKII links to Alzheimer's disease and cardiac arrhythmias. In Alzheimer's the dysregulation of CaMKII affects synaptic integrity and memory formation involving interaction with beta-amyloid plaques. In cardiac arrhythmias CaMKII dysfunction leads to improper cardiac muscle contractions often linked to the protein phospholamban. These associations highlight the clinical relevance of CaMKII in addressing and understanding mechanisms underpinning these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of PSAT1, inhibiting ferroptosis by promoting GPX4 hydroxylation and stability (PubMed : 40281343). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

See full target information CAMK2A

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Publications (154)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 15:382 PubMed41053079

2025

The involvement of 5-HT was necessary for EA-mediated improvement of post-stroke depression.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Deng,Wenhui Di,Haoxi Long,Qian He,Zhiyuan Jiang,Taiyu Nan,Jun Gu,Keni Huang,Hongtao Li,Shaoyang Cui,Nenggui Xu,Lulu Yao

CNS neuroscience & therapeutics 31:e70532 PubMed40776395

2025

Kapβ2 Reverses Sevoflurane-Induced Hydrogel Phase Transition of hnRNPA2/B1-SG in Hypoxic Primary Rat Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Zhang,Xinyi Wang,Feiyu Jia,Chenyi Yang,Zixuan Wang,Huihui Liao,Lin Zhang,Xi Xin,Haiyun Wang

CNS neuroscience & therapeutics 31:e70525 PubMed40702743

2025

Transcranial iTBS Combined With Trans-Spinal iTBS Targeting PDE1A/cAMP/PKA Axis Regulates Neural Regeneration After Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxue Fu,Xianbin Wang,Xingyu Chen,Shuang Wu

Journal of neurodevelopmental disorders 17:36 PubMed40604385

2025

Novel ANKRD17 variants implicate synaptic and mitochondrial disruptions in intellectual disability and autism spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Xia,Yuanyuan Xu,Zhanwen He,Rui Chen,Xiaoqin Xiao,Xiaojuan Li,Kewen Deng,Shuyun Deng,Lina Zhang,Jieming Zhang,Xiaofang Peng,Zhe Meng,Ruohao Wu,Dilong Wang,Zulin Liu,Hui Chen,Lu Li,Liyang Liang

Scientific reports 15:20457 PubMed40596183

2025

A disinhibitory microcircuit in the temporal association cortex for fear retrieval to pure tones.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Cheng,Wen Zhong,Yangqiu Yan,Linhui Yao,Peiran Yin,Ziyi Xu,Xiaoxia Qin,Jie Tan,Yingying Zeng,Jinhua Liu,Zhongju Xiao

Cellular and molecular life sciences : CMLS 82:259 PubMed40579608

2025

Piezo1 disrupts blood-brain barrier via CaMKII/Nrf2 in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Fu,Yang Yu,Shangyuan Wang,Peixian Xu,Yuting Sun,Jiaqi Li,Xiaoli Ge,Shuming Pan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e01182 PubMed40387368

2025

Locus Coeruleus Noradrenergic-Spinal Projections Contribute to Electroacupuncture-Mediated Antinociception in Postoperative Pain in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Guang Chu,Ru Zhang,Hai-Tao Li,Ying-Chun Li,Hui Ding,Zhen-Zhen Li,Wen-Juan Han,Fei Wang,Xing-Xing Zheng,Hong-Hui Mao,Hua Yuan,Sheng-Xi Wu,Rou-Gang Xie,Ceng Luo

Frontiers in cell and developmental biology 13:1552282 PubMed40241796

2025

Heat shock affects the Ca/calmodulin-dependent protein kinase II dynamic during bovine sperm capacitation and acrosome reaction.

Applications

Unspecified application

Species

Unspecified reactive species

Thais de Sousa Santos,Isabelle Scarpini Contrim,Daniela Franco da Silva,Mayra Elena Ortiz D'Avila Assumpção,Fabiola Freitas de Paula-Lopes,Weber Beringui Feitosa

Cell death discovery 11:166 PubMed40216750

2025

MGP regulates the adipogenic differentiation of mesenchymal stem cells in osteoporosis via the Ca2+/CaMKII/RIP140/FABP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglang Wang,Yunhui Zhang,Chenhao Xu,Quanfeng Li,Pengfei Ji,Huatao Liu,Yibin Zhang,Jiahao Jin,Zihao Yuan,Miao Yuan,Pei Feng,Yanfeng Wu,Wenjie Liu,Huiyong Shen,Peng Wang

Nature communications 16:2941 PubMed40140405

2025

Evolutionary divergence in CTCF-mediated chromatin topology drives transcriptional innovation in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Wu,Dan Xiong,Rong Liu,Xingqiang Lai,Yuhan Tian,Ziying Xie,Li Chen,Lanqi Hu,Jingjing Duan,Xinyu Gao,Xian Zeng,Wei Dong,Ting Xu,Fang Fu,Xin Yang,Xinlai Cheng,Dariusz Plewczynski,Minji Kim,Wenjun Xin,Tianyun Wang,Andy Peng Xiang,Zhonghui Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CaMKII antibody [EP1829Y]

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (AB52476)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [EP1829Y] (AB52476)

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII antibody [EP1829Y] (AB52476)

Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476)

Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476)

Western blot - Anti-CaMKII antibody [EP1829Y] (AB52476)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (154)

Product promise