Anti-CaMKII beta antibody [EPR28076-54]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling CaMKII beta with ab314897 at 1/500 (1.05 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human astrocytoma (PMID : 30816530). The section was incubated with ab314897 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling CaMKII beta with ab314897 at 1/500 (1.05 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with ab314897 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CaMKII beta with ab314897 at 1/500 (1.05 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human liver. The section was incubated with ab314897 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling CaMKII beta with ab314897 at 1/5000 (0.105 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum (PMID : 29784938). The section was incubated with ab314897 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CaMKII beta with ab314897 at 1/5000 (0.105 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse liver. The section was incubated with ab314897 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells labelling CaMKII beta with ab314897 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse small intestine tissue staining CaMKII beta with ab314897 at a 1/5000 dilution, ab315150 anti-Serotonin used at 1/5000 dilution and ab238123 anti-MPTX used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-CaMKII beta (green; Opal^™690), anti-Serotonin (magenta; Opal^™520) and anti-MPTX (grey; Opal^™570) on mouse small intestine.
Panel B : anti-CaMKII beta staining myenteric nerve plexus and endocrine cells in mouse small intestine.
Panel C : anti-Serotonin staining enterochromaffin cells in mouse small intestine.
Panel D : anti-MPTX staining membrane of Paneth cells in mouse small intestine.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining : in the order of ab314897, ab315150 and ab238123 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CaMKII beta with ab314897 at 1/5000 (0.105 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat liver. The section was incubated with ab314897 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CaMKII beta with ab314897 at 1/5000 (0.105 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum (PMID : 21752990; PMID : 31064859). The section was incubated with ab314897 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse stomach tissue staining CaMKII beta with ab314897 at 1/5000 dilution, ab315150 anti-Serotonin used at 1/5000 dilution and ab287960 anti-Aurora B used at a 1/300 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CaMKII beta (magenta; Opal^™690), anti-Serotonin (green; Opal^™520) and anti-Aurora B (gray; Opal^™570) on mouse stomach.
Panel B : anti-CaMKII beta staining endocrine cells in mouse stomach.
Panel C : anti-Serotonin staining enterochromaffin cells in mouse stomach.
Panel D : anti-Aurora B staining proliferating cells in mouse stomach.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314897, ab315150 and ab287960 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse small intestine tissue staining Eph receptor B2 with ab252935 at 1/500 dilution, ab314897 anti-CaMKII beta used at 1/5000 dilution and ab238123 anti-MPTX used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-Eph receptor B2 (green; Opal™520), anti-CaMKII beta (magenta; Opal™690) and anti-MPTX (gray; Opal™570) on mouse small intestine.
Panel B : anti-Eph receptor B2 staining membrane of stem cells in mouse small intestine.
Panel C : anti-CaMKII beta staining endocrine cells in mouse small intestine.
Panel D : anti-MPTX staining membrane of Paneth cells in mouse small intestine.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab259935, ab314897 and ab238123 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse stomach tissue staining CaMKII beta with ab314897 at 1/5000 dilution, ab315150 anti-Serotonin used at 1/5000 dilution and ab209790 anti-Ghrelin used at a 1/40000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-CaMKII beta (magenta; Opal^™690), anti-Serotonin (green; Opal^™520) and anti-Ghrelin (gray; Opal^™570) on mouse stomach.
Panel B : anti-CaMKII beta staining endocrine cells in mouse stomach.
Panel C : anti-Serotonin staining enterochromaffin cells in mouse stomach.
Panel D : anti-Ghrelin staining ghrelin in mouse stomach.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314897, ab315150 and ab209790 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• WB

Supplier Data

Western blot - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-CaMKII beta antibody [EPR28076-54] - (ab314897) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (<a href='/en-us/products/primary-antibodies/camk2b-2d-2g-phospho-t287-camk2a-phospho-t286-antibody-epr28075-37-ab320638'>ab320638</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate (untreated membrane) at 20 µg

Lane 2:

Human cerebellum tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50-60 kDa,60 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : spleen (PMID : 1321343), liver(PMID : 1321343).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CaMKII beta antibody [EPR28076-54] (ab314897) at 1/1000 dilution

Lane 1:

Human hippocampus tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 29615706). This antibody can recognize CaMKII beta, CaMKII alpha, CaMKII delta and CaMKII gamma. They have many isofroms and isoforms have different molecular weight.

In Western blot, Anti-CaMKII beta antibody [EPR28076-54] - (ab314897) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (<a href='/en-us/products/primary-antibodies/camk2b-2d-2g-phospho-t287-camk2a-phospho-t286-antibody-epr28075-37-ab320638'>ab320638</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Mouse brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-60 kDa,60 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : spleen (PMID : 1321343), liver(PMID : 1321343).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CaMKII beta antibody [EPR28076-54] (ab314897) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat hippocampus tissue lysate at 20 µg

Lane 7:

Rat liver tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 29615706). This antibody can recognize CaMKII beta, CaMKII alpha, CaMKII delta and CaMKII gamma. They have many isofroms and isoforms have different molecular weight.

In Western blot, Anti-CaMKII beta antibody [EPR28076-54] - (ab314897) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (<a href='/en-us/products/primary-antibodies/camk2b-2d-2g-phospho-t287-camk2a-phospho-t286-antibody-epr28075-37-ab320638'>ab320638</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Rat brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-60 kDa,60 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-CaMKII beta antibody [EPR28076-54] - (ab314897) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-CAMK2B/2D/2G (phospho T287) + CAMK2A (phospho T286) antibody [EPR28075-37] (<a href='/en-us/products/primary-antibodies/camk2b-2d-2g-phospho-t287-camk2a-phospho-t286-antibody-epr28075-37-ab320638'>ab320638</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a human CaMKII beta (T287A) expression vector containing a myc-His-tag® whole cell lysate (untreated membrane) at 10 µg

Lane 2:

293T cells transfected with a human wild-type CaMKII beta expression vector containing a myc-His-tag® whole cell lysate (untreated membrane) at 10 µg

Lane 3:

293T cells transfected with a human wild-type CaMKII beta expression vector containing a myc-His-tag® whole cell lysate (alkaline phosphatase treated membrane) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50-60 kDa,80 kDa

false

Exposure time: 15s

• Dot

Supplier Data

Dot Blot - Anti-CaMKII beta antibody [EPR28076-54] (AB314897)

Dot blot analysis of CaMKII beta using ab314897 at 1 : 1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 180 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST