Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free (AB191588)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling CaMKII delta with Purified ab181052 at 1 : 100 dilution (1.82 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181052).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free (AB191588)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling CaMKII delta with Purified ab181052 at 1 : 100 dilution (1.82 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181052).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free (AB191588)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue sections labeling CaMKII delta using unpurified ab181052 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181052).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free (AB191588)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181052).

Lanes 1- 2 : Merged signal (red and green). Green - ab181052 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab181052 was shown to react with CaM-kinase II in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab267322 (knockout cell lysate ab257376) was used. Wild-type HEK-293T and CAMK2D knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab181052 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (<a href='/en-us/products/primary-antibodies/camkii-delta-antibody-epr13095-ab181052'>ab181052</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

CAMK2D knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human CAMK2D (CaMKII delta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-camk2d-camkii-delta-knockout-hek-293t-cell-line-ab267322'>ab267322</a>)

Predicted band size: 56 kDa

Observed band size: 50 kDa

false

• WB

Lab

Western blot - Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free (AB191588)

This WB data was generated using the same anti-CAKII delta antibody clone [EPR13095] in a different buffer formulation (cat# ab181052).

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CaMKII delta knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : A431 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (<a href='/en-us/products/primary-antibodies/camkii-delta-antibody-epr13095-ab181052'>ab181052</a>)

Predicted band size: 56 kDa

false

• WB

Lab

Western blot - Anti-CaMKII delta antibody [EPR13095] - BSA and Azide free (AB191588)

This data was developed using ab181052, the same antibody clone in a different buffer formulation.

ab181052 was shown to react with CAMK2D in wild-type HAP1 cells in Western blot with loss of signal observed in a CAMK2D knockout cell line. Wild-type HAP1 and CAMK2D knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab181052 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-CaMKII delta antibody [EPR13095] (<a href='/en-us/products/primary-antibodies/camkii-delta-antibody-epr13095-ab181052'>ab181052</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 40 µg

Lane 2:

CAMK2D knock-out HAP1 lysate at 40 µg

false