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AB201966

Anti-CamKII gamma antibody [8G10C1]

1

(1 Review)

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(3 Publications)

Mouse Monoclonal CamKII gamma antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CAMK2G aa 300-500.

View Alternative Names

CAMK, CAMK-II, CAMKG, CAMK2G, Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CaMK-II subunit gamma

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (AB201966)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (AB201966)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (AB201966)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (AB201966)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining.

Flow Cytometry - Anti-CamKII gamma antibody [8G10C1] (AB201966)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CamKII gamma antibody [8G10C1] (AB201966)

Flow cytometric analysis of Jurkat cells labeling CamKII gamma with ab201966 at 1/200 dilution (green). Negative control (red).

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)
  • WB

Unknown

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)

Lanes 1-3 : Merged signal (red and green). Green - ab201966 observed at 63 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab201966 Anti-CamKII gamma antibody [8G10C1] was shown to specifically react with CamKII gamma in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264712 (knockout cell lysate ab258340) was used. Wild-type and CamKII gamma knockout samples were subjected to SDS-PAGE. ab201966 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CAMK2G knockout HeLa cell lysate at 20 µg

Lane 3:

T-47D cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 63 kDa

false

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)
  • WB

Supplier Data

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)

All lanes:

Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

Lane 1:

PC12 cell lysate

Lane 2:

Jurkat cell lysate

Lane 3:

T47D cell lysate

Lane 4:

HepG2 cell lysate

Predicted band size: 63 kDa

false

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)
  • WB

Lab

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)

Lanes 1-3 : Merged signal (red and green). Green - ab201966 observed at 63 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab201966 Anti-CamKII gamma antibody [8G10C1] was shown to specifically react with CamKII gamma in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264712 (knockout cell lysate ab258340) was used. Wild-type and CamKII gamma knockout samples were subjected to SDS-PAGE. ab201966 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CAMK2G knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CAMK2G (CamKII gamma) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-camk2g-camkii-gamma-knockout-hela-cell-line-ab264712'>ab264712</a>)

Lane 3:

T-47D cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 63 kDa

false

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)
  • WB

Supplier Data

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)

(Expected MW is 44 kDa)

All lanes:

Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

All lanes:

Human recombinant CamKII gamma (aa322-481) protein

Predicted band size: 63 kDa

false

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)
  • WB

Supplier Data

Western blot - Anti-CamKII gamma antibody [8G10C1] (AB201966)

All lanes:

Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 63 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8G10C1

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Human

Applications

WB, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CAMK2G aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q13555

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

This product was changed from ascites to supernatant. Lot no's high than GR280299-5 are from Tissue Culture Supernatant
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity (PubMed : 16690701). In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin (PubMed : 16690701). In the central nervous system, it is involved in the regulation of neurite formation and arborization (PubMed : 30184290). It may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity).
See full target information CAMK2G
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1871 PubMed39984440

2025

Activation of CAMK2 by pseudokinase PEAK1 represents a targetable pathway in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yang,Xiuquan Ma,Tianyue Zhao,David R Croucher,Elizabeth V Nguyen,Kimberley C Clark,Changyuan Hu,Sharissa L Latham,Charles Bayly-Jones,Bao V Nguyen,Srikanth Budnar,Sung-Young Shin,Lan K Nguyen,Thomas R Cotton,Anderly C Chüeh,Terry C C Lim Kam Sian,Margaret M Stratton,Andrew M Ellisdon,Roger J Daly

Nucleic acids research 50:5263-5281 PubMed35489070

2022

Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Boudreault,Mathieu Durand,Carole-Anne Martineau,Jean-Pierre Perreault,Guy Lemay,Martin Bisaillon

Scientific reports 9:9998 PubMed31292492

2019

Characterisation of a human antibody that potentially links cytomegalovirus infection with systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ying Jacklyn Neo,Seng Yin Kelly Wee,Isabelle Bonne,Sen Hee Tay,Manfred Raida,Vojislav Jovanovic,Anna-Marie Fairhurst,Jinhua Lu,Brendon J Hanson,Paul A MacAry
View all publications
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Product promise

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