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Mouse Monoclonal KCC2A phospho T286 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat, Human samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha phospho T286 aa 250-300.


Images

Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha phospho T286 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link P11275

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Expected
Mouse
Tested
Expected
Rat
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Species

Human

Dilution info

1/100

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/2000

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed:14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed:11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed:35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

Alternative names

Recommended products

Mouse Monoclonal KCC2A phospho T286 antibody. Suitable for ICC/IF, WB and reacts with Mouse, Rat, Human samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha phospho T286 aa 250-300.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha phospho T286 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link P11275
Clone number

22B1

Purification technique

Affinity purification Protein G

Specificity

ab171095 detects the phosphorylated alpha subunit CaMKII only. It does not detect the non-phosphorylated alpha subunit CaMKII or the ~60 kDa beta subunit in either phosphorylation state.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CaMKII also known as calcium/calmodulin-dependent protein kinase II is an enzyme functioning critically in cellular signaling. It consists of multiple isoforms including alpha beta gamma and delta with the delta isoform Delta 1829 frequently highlighted for its specific roles. The molecular weight of CaMKII is approximately 50 to 60 kDa varying slightly between isoforms. CaMKII is widely expressed in the brain but is also present in the heart skeletal muscle and other tissues. Its structure includes a catalytic domain regulatory segment and association domain which allows auto-regulation and interaction with other molecules.

Biological function summary

CaMKII plays a vital role in cellular processes such as learning memory and muscle contraction. It can form a complex structure involving multiple subunits which allows for its unique ability to signal in response to calcium influx and calmodulin binding. This causes autophosphorylation of CaMKII allowing it to remain active even when calcium levels diminish. This property makes CaMKII essential for long-term potentiation (LTP) in neuron synapses influencing cognitive functions deeply.

Pathways

CaMKII is an important component of both the calcium signaling and synaptic plasticity pathways. In the calcium signaling pathway CaMKII interacts with calmodulin and other kinases to modulate calcium entry and signaling. In synaptic plasticity CaMKII works closely with proteins like NMDA receptors which regulate synaptic strength and modulation essential for encoding learning and memory. These interactions highlight its significance as a mediator between signaling cascades and cellular responses.

Associated diseases and disorders

Abnormal regulation or expression of CaMKII links to Alzheimer's disease and cardiac arrhythmias. In Alzheimer's the dysregulation of CaMKII affects synaptic integrity and memory formation involving interaction with beta-amyloid plaques. In cardiac arrhythmias CaMKII dysfunction leads to improper cardiac muscle contractions often linked to the protein phospholamban. These associations highlight the clinical relevance of CaMKII in addressing and understanding mechanisms underpinning these diseases.

Product promise

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5 product images

  • Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095), expandable thumbnail

    Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Lanes 1 - 2: Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) at 1/2000 dilution

    Lanes 3 - 4: A non phosphorylated CaMKII antibody at 1/2000 dilution

    Lane 1: Unphosphorylated rat brain homogenate

    Lanes 2 and 4: Phosphorylated Rat brain homogenate

    Lane 3: Unphosphorylated Rat brain homogenate

    Predicted band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Immunofluorescence analysis of U251 (human) cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Immunofluorescence analysis of Neuro-2 (mouse) cells (left image) or cells stimulated with Lambda-phosphatase (right image) labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. F-actin (red) and nuclei (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Immunofluorescence analysis of HeLa cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. Nuclei are stained (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095)

    Immunofluorescence analysis of C6 cells labeling phospohrylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).

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Product protocols

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