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AB171095

Anti-CaMKII (phospho T286) antibody [22B1]

4

(2 Reviews)

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(39 Publications)

Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) is a mouse monoclonal antibody detecting CaMKII in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 20 publications

View Alternative Names

CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha

5 Images
Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)
  • WB

Supplier Data

Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)

Lanes 1 - 2:

Western blot - Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) at 1/2000 dilution

Lanes 3 - 4:

A non phosphorylated CaMKII antibody at 1/2000 dilution

Lane 1:

Unphosphorylated rat brain homogenate

Lanes 2 and 4:

Phosphorylated Rat brain homogenate

Lane 3:

Unphosphorylated Rat brain homogenate

Predicted band size: 54 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)

Immunofluorescence analysis of U251 (human) cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)

Immunofluorescence analysis of HeLa cells labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. Nuclei are stained (blue).

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)

Immunofluorescence analysis of C6 cells labeling phospohrylated CaMKII (green) with ab171095 at a 1/100 dilution. Nuclei are stained (blue).

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CaMKII (phospho T286) antibody [22B1] (AB171095)

Immunofluorescence analysis of Neuro-2 (mouse) cells (left image) or cells stimulated with Lambda-phosphatase (right image) labeling phosphorylated CaMKII (green) with ab171095 at a 1/50 dilution. F-actin (red) and nuclei (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

22B1

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha phospho T286 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P11275

Specificity

ab171095 detects the phosphorylated alpha subunit CaMKII only. It does not detect the non-phosphorylated alpha subunit CaMKII or the ~60 kDa beta subunit in either phosphorylation state.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of CaMKII?
Anti-CaMKII (phospho T286) [22B1] (ab171095) specifically detects a band for CaMKII (UniProt: P11275) at a molecular weight of 54kDa.

Trusted by the scientific community
Anti-CaMKII (phospho T286) [22B1] (ab171095) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII also known as calcium/calmodulin-dependent protein kinase II is an enzyme functioning critically in cellular signaling. It consists of multiple isoforms including alpha beta gamma and delta with the delta isoform Delta 1829 frequently highlighted for its specific roles. The molecular weight of CaMKII is approximately 50 to 60 kDa varying slightly between isoforms. CaMKII is widely expressed in the brain but is also present in the heart skeletal muscle and other tissues. Its structure includes a catalytic domain regulatory segment and association domain which allows auto-regulation and interaction with other molecules.
Biological function summary

CaMKII plays a vital role in cellular processes such as learning memory and muscle contraction. It can form a complex structure involving multiple subunits which allows for its unique ability to signal in response to calcium influx and calmodulin binding. This causes autophosphorylation of CaMKII allowing it to remain active even when calcium levels diminish. This property makes CaMKII essential for long-term potentiation (LTP) in neuron synapses influencing cognitive functions deeply.

Pathways

CaMKII is an important component of both the calcium signaling and synaptic plasticity pathways. In the calcium signaling pathway CaMKII interacts with calmodulin and other kinases to modulate calcium entry and signaling. In synaptic plasticity CaMKII works closely with proteins like NMDA receptors which regulate synaptic strength and modulation essential for encoding learning and memory. These interactions highlight its significance as a mediator between signaling cascades and cellular responses.

Abnormal regulation or expression of CaMKII links to Alzheimer's disease and cardiac arrhythmias. In Alzheimer's the dysregulation of CaMKII affects synaptic integrity and memory formation involving interaction with beta-amyloid plaques. In cardiac arrhythmias CaMKII dysfunction leads to improper cardiac muscle contractions often linked to the protein phospholamban. These associations highlight the clinical relevance of CaMKII in addressing and understanding mechanisms underpinning these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
See full target information CAMK2A phospho T286
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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1611226 PubMed40530334

2025

Induced neural stem cells ameliorate blood-brain barrier injury by modulating the calcium signaling pathway of astrocyte in cerebral ischemia-reperfusion rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyun Liang,Chuanshang Cao,Ningmei Liu,Dongmei Chen,Ting Liu,Haibin Ma,Jiaxin Liu,Taojuan Wu,Jianguo Niu

Frontiers in pharmacology 16:1557685 PubMed40206075

2025

Allicin ameliorates acute myocardial infarction in rats by modulating calcium homeostasis in cardiomyocytes through the induction of hydrogen sulfide production.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyu Liu,Shaojun Xu,Juan Wang,Xinxia Li,Ruiting Liu,Le Zhao,Yikui Li,Rongmei Shi,Jinyan Zhang

Journal of molecular and cellular cardiology plus 9:100084 PubMed39803591

2025

Cardiac growth patterns and metabolism before and after birth in swine: Role of miR in proliferation, hypertrophy and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine G Dimasi,Jack R T Darby,Stacey L Holman,Megan Quinn,Ashley S Meakin,Mike Seed,Michael D Wiese,Janna L Morrison

Journal of pharmaceutical analysis 14:101149 PubMed39720622

2024

Depression of Ca1.2 activation and expression in mast cells ameliorates allergic inflammation diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjing Zhang,Yingnan Zeng,Haoyun Bai,Wen Zhang,Zhuoyin Xue,Shiling Hu,Shemin Lu,Nan Wang

Aging cell 24:e14440 PubMed39610146

2024

Piezo1 exacerbates inflammation-induced cartilaginous endplate degeneration by activating mitochondrial fission via the Ca/CaMKII/Drp1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhidi Lin,Guangyu Xu,Xiao Lu,Hongli Wang,Feizhou Lu,Xinlei Xia,Jian Song,Jianyuan Jiang,Xiaosheng Ma,Fei Zou

Aging cell 24:e14349 PubMed39380368

2024

CaMKIIα-TARPγ8 signaling mediates hippocampal synaptic impairment in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao JianHua,MingCan Li,Qilin Hu,Peter Donoghue,Sanwei Jiang,Junmei Li,Songji Li,Xinyi Ren,Ziyuan Zhang,Jingzhi Du,Yi Yu,Paul Chazot,Chengbiao Lu

The journal of headache and pain 25:140 PubMed39192198

2024

Ventral posteromedial nucleus of the thalamus gates the spread of trigeminal neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Du,Shi-Da Lin,Xue-Qing Wu,Bao-Yu Xue,Yi-La Ding,Jia-Hang Zhang,Bei Tan,Guo-Dong Lou,Wei-Wei Hu,Zhong Chen,Shi-Hong Zhang

Renal failure 46:2387432 PubMed39177245

2024

Verapamil inhibits inflammation and promotes autophagy to alleviate ureteral scar by regulation of CaMK IIδ/STAT3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingqiang Zeng,Zhiwei Zhu,Wuxiong Yuan,Zhengyan Tang,Zhibiao Qing,Qiang Lu,Xuecheng Wu,Junhuan He,Yuanwei Li,Zhuo Li

Gene 929:148812 PubMed39116959

2024

Cav3.2 deletion attenuates nonalcoholic fatty liver disease in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Li,Chengyun Hu,Shanshan Luo,Feibiao Dai,Chuanyao Li,Wanjun Zhou,Jiawu Wang,Hao Chen,Zhen Wang,Tengfei Long,Lai Jiang,Chaoliang Tang

The Journal of physiology 602:4251-4269 PubMed39087821

2024

Maternal high fat-high energy diet alters metabolic factors in the non-human primate fetal heart.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie R Bertossa,Jack R T Darby,Stacey L Holman,Ashley S Meakin,Cun Li,Hillary F Huber,Michael D Wiese,Peter W Nathanielsz,Janna L Morrison
View all publications
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