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AB32678

Anti-CaMKII (phospho T286) antibody

5

(28 Reviews)

|

(88 Publications)

Anti-CaMKII (phospho T286) antibody (ab32678) is a rabbit polyclonal antibody detecting CaMKII in Western Blot. Suitable for Rat.

- Over 80 publications
- Trusted since 2006

View Alternative Names

Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, Camk2a

1 Images
Western blot - Anti-CaMKII (phospho T286) antibody (AB32678)
  • WB

Unknown

Western blot - Anti-CaMKII (phospho T286) antibody (AB32678)

Predicted molecular weight : ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
These images show the phosphospecificity of this antibody.

All lanes:

Western blot - Anti-CaMKII (phospho T286) antibody (ab32678) at 1/1000 dilution

Lane 1:

Rat brain cortex lysate. at 50 µg

Lane 2:

Rat brain cortex lysate preincubated with lambda-phosphatase. at 50 µg

Predicted band size: 54 kDa

Observed band size: 50 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Rat Camk2a phospho T286. The exact immunogen used to generate this antibody is proprietary information.

P11275

Specificity

This antibody is specific for the ~50 kDa alpha CaM Kinase II subunit and the ~60 kDa beta CaM Kinase II subunit phosphorylated at Thr286 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

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Reactivity data

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Have you thought about this alternative?

AB134041

Anti-CaMKII antibody [EPR6686(2)]

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Product details

What is this antibody validated in?
Anti-CaMKII (phospho T286) antibody (ab32678) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Rat samples.

What is the molecular weight of CaMKII?
Anti-CaMKII (phospho T286) (ab32678) specifically detects a band for CaMKII (UniProt: Q13554) at a molecular weight of 50kDa.

Trusted by the scientific community
Anti-CaMKII (phospho T286) (ab32678) was first used in a scientific publication in 2006 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-CaMKII (phospho T286) (ab32678) has over 25 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CaMKII also known as calcium/calmodulin-dependent protein kinase II is an enzyme functioning critically in cellular signaling. It consists of multiple isoforms including alpha beta gamma and delta with the delta isoform Delta 1829 frequently highlighted for its specific roles. The molecular weight of CaMKII is approximately 50 to 60 kDa varying slightly between isoforms. CaMKII is widely expressed in the brain but is also present in the heart skeletal muscle and other tissues. Its structure includes a catalytic domain regulatory segment and association domain which allows auto-regulation and interaction with other molecules.
Biological function summary

CaMKII plays a vital role in cellular processes such as learning memory and muscle contraction. It can form a complex structure involving multiple subunits which allows for its unique ability to signal in response to calcium influx and calmodulin binding. This causes autophosphorylation of CaMKII allowing it to remain active even when calcium levels diminish. This property makes CaMKII essential for long-term potentiation (LTP) in neuron synapses influencing cognitive functions deeply.

Pathways

CaMKII is an important component of both the calcium signaling and synaptic plasticity pathways. In the calcium signaling pathway CaMKII interacts with calmodulin and other kinases to modulate calcium entry and signaling. In synaptic plasticity CaMKII works closely with proteins like NMDA receptors which regulate synaptic strength and modulation essential for encoding learning and memory. These interactions highlight its significance as a mediator between signaling cascades and cellular responses.

Abnormal regulation or expression of CaMKII links to Alzheimer's disease and cardiac arrhythmias. In Alzheimer's the dysregulation of CaMKII affects synaptic integrity and memory formation involving interaction with beta-amyloid plaques. In cardiac arrhythmias CaMKII dysfunction leads to improper cardiac muscle contractions often linked to the protein phospholamban. These associations highlight the clinical relevance of CaMKII in addressing and understanding mechanisms underpinning these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (PubMed : 15312654). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (By similarity). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (By similarity). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
See full target information Camk2a phospho T286
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Publications (88)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7000 PubMed39143095

2024

Lamin A/C deficiency-mediated ROS elevation contributes to pathogenic phenotypes of dilated cardiomyopathy in iPSC model.

Applications

WB

Species

Human

Hangyuan Qiu,Yaxun Sun,Xiaochen Wang,Tingyu Gong,Jun Su,Jiaxi Shen,Jingjun Zhou,Jiafeng Xia,Hao Wang,Xiangfu Meng,Guosheng Fu,Donghui Zhang,Chenyang Jiang,Ping Liang

Biological research 56:65 PubMed38041203

2023

Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyi Ding,Long Li,Sinuo Wang,Yajun Cao,Minguang Yang,Yaling Dai,Huawei Lin,Jianhong Li,Yulu Liu,Zhifu Wang,Weilin Liu,Jing Tao

International journal of molecular sciences 24: PubMed38003332

2023

Ketogenic Diet Regulates Cardiac Remodeling and Calcium Homeostasis in Diabetic Rat Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-I Lee,Nguyen Ngoc Trang,Ting-Wei Lee,Satoshi Higa,Yu-Hsun Kao,Yao-Chang Chen,Yi-Jen Chen

Diabetology & metabolic syndrome 15:217 PubMed37891701

2023

Glucose fluctuations aggravate myocardial fibrosis via activating the CaMKII/Stat3 signaling in type 2 diabtetes.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Huan-Huan Liu,Fan Yang,Zhi-Yuan Zhang,Zhen-Ye Zhang,Xiao-Xi Zhao,Ling-Ling Qian,Shi-Peng Dang,Ru-Xing Wang

Scientific reports 13:13927 PubMed37626185

2023

PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Duk-Shin Lee,Tae-Hyun Kim,Hana Park,Ji-Eun Kim

International journal of biological sciences 19:2934-2956 PubMed37324937

2023

Colchicine attenuates the electrical remodeling of post-operative atrial fibrillation through inhibited expression of immune-related hub genes and stabilization of microtubules.

Applications

Unspecified application

Species

Unspecified reactive species

Hangying Ying,Wenpu Guo,Xiaomei Tang,Jun Pan,Pengcheng Yu,Hangping Fan,Xiaochen Wang,Ruhong Jiang,Chenyang Jiang,Ping Liang

Frontiers in molecular neuroscience 16:1107355 PubMed36846565

2023

Neural mechanism underlies CYLD modulation of morphology and synaptic function of medium spiny neurons in dorsolateral striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Yi Tan,Jin-Xiang Jiang,Hui-Xian Huang,Xiu-Ping Mo,Jing-Ru Feng,Yu Chen,Li Yang,Cheng Long

Journal of molecular and cellular cardiology 175:49-61 PubMed36528076

2022

CaMKII and reactive oxygen species contribute to early reperfusion arrhythmias, but oxidation of CaMKIIδ at methionines 281/282 is not a determining factor.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Haugsten Hansen,Mani Sadredini,Almira Hasic,Mark E Anderson,Ivar Sjaastad,Mathis Korseberg Stokke

Frontiers in molecular neuroscience 15:868583 PubMed36147210

2022

Behavioral and Molecular Responses to Exogenous Cannabinoids During Pentylenetetrazol-Induced Convulsions in Male and Female Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Zirotti Rosenberg,Maxs Méndez-Ruette,Mario Gorziglia,Benjamín Alzerreca,Javiera Cabello,Sofía Kaufmann,Lukas Rambousek,Andrés Iturriaga Jofré,Ursula Wyneken,Carlos A Lafourcade

Experimental & molecular medicine 54:1109-1124 PubMed35922532

2022

Suppression of exaggerated NMDAR activity by memantine treatment ameliorates neurological and behavioral deficits in aminopeptidase P1-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Soo Bae,Sang Ho Yoon,Young Sook Kim,Sung Pyo Oh,Woo Seok Song,Jin Hee Cha,Myoung-Hwan Kim
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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