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AB3557

Anti-CAMKIV antibody

4

(4 Reviews)

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(14 Publications)

Rabbit Polyclonal CAMKIV antibody. Suitable for ICC, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Rat, Primates, Mouse, Plants, Xenopus laevis, Turtle, Dog samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Mouse Camk4 aa 100-150.

View Alternative Names

Calcium/calmodulin-dependent protein kinase type IV, CaMK IV, CaM kinase-GR

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody (AB3557)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody (AB3557)

Immunofluorescence analysis of CaM Kinase IV in SH-SY5Y cells using ab3557. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab3557 at 2 μg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by Alexa Fluor® 488 conjugate Goat anti-Rabbit IgG (H+L) at a 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody (AB3557)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody (AB3557)

Immunohistochemistry analysis of CaM Kinase IV using ab3557 at 1/100 dilution for 1 hour at 37°C showing staining in the cytoplasm and nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left) followed by an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody (AB3557)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody (AB3557)

Immunohistochemistry analysis of CaM Kinase IV using ab3557 at 1/20 dilution for 1 hour at 37°C showing staining in the cytoplasm and nucleus of paraffin-embedded rat brain tissue (right) compared to a negative control without primary antibody (left) followed by an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature.

Western blot - Anti-CAMKIV antibody (AB3557)
  • WB

Supplier Data

Western blot - Anti-CAMKIV antibody (AB3557)

A ~ 59 kDa band corresponding to CaM Kinase IV was observed across cell lines and tissues tested. Additionally ~25 kDa band was observed in PC12 cell line.

Blocking buffer : 5 % skimmed milk

All lanes:

Western blot - Anti-CAMKIV antibody (ab3557) at 1 µg/mL

Lane 1:

PC12 at 30 µg

Lane 2:

Rat Brain at 30 µg

Lane 3:

Mouse Brain at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

Predicted band size: 52 kDa

false

Western blot - Anti-CAMKIV antibody (AB3557)
  • WB

Supplier Data

Western blot - Anti-CAMKIV antibody (AB3557)

A 59 kDa band corresponding to CaMKIV was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be CaMKIV negative.

All lanes:

Western blot - Anti-CAMKIV antibody (ab3557) at 1 µg/mL

Lane 1:

Mouse Brain tissue extract at 30 µg

Lane 2:

Rat Brain tissue extract at 30 µg

Lane 3:

Rat Liver tissue extract at 30 µg

Lane 4:

Rat Kidney tissue extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution

Predicted band size: 52 kDa

Observed band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Plants, Primates, Dog, Turtle, Xenopus laevis

Applications

WB, ICC, ICC/IF, IHC-P, IHC-Fr

applications

Immunogen

Synthetic Peptide within Mouse Camk4 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P08414

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Reactivity data

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1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p>This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Plants": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Primates": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "0.5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p>This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Turtle": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Xenopus laevis": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as CaMKIV (Calcium/Calmodulin-dependent Protein Kinase IV) plays a role in various cellular processes. Mechanically CaMKIV is a serine/threonine protein kinase with a molecular weight of approximately 52 kDa. It depends on calcium ions and calmodulin for activation. The protein is expressed in several tissues including the brain thymus and testis. It is known for its involvement in phosphorylating substrates which then activate transcription factors.
Biological function summary

CaMKIV takes part in the regulation of gene expression and is involved in neural signaling cell cycle control and immune responses. This kinase forms part of a larger signaling complex that facilitates specific cellular events. Its activity affects processes such as long-term potentiation and T-cell maturation indicating its significance in both the nervous and immune systems.

Pathways

CaMKIV integrates into several signaling pathways including the calcium signaling pathway and CREB (cAMP response element-binding protein) pathway. Through these pathways CaMKIV activates CREB via phosphorylation linking it to gene transcription processes essential for memory formation. CaMKIV acts alongside proteins like other CaM kinases such as CaMKI and CaMKII to ensure efficient signal transduction.

CaMKIV's function impacts conditions like neurodegenerative diseases and autoimmune disorders. Dysregulation of CaMKIV may relate to Alzheimer's disease where altered calcium homeostasis contributes to pathogenesis. The protein also connects to autoimmune diseases through its interaction with NFAT (nuclear factor of activated T-cells) which influences immune responses and might lead to disorders when dysregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18 (By similarity). May be involved in spermatogenesis.
See full target information Camk4
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 22:1107-1117 PubMed34385713

2021

Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Pengchong Li,Mengdi Jiang,Ketian Li,Hao Li,Yangzhong Zhou,Xinyue Xiao,Yue Xu,Suzanne Krishfield,Peter E Lipsky,George C Tsokos,Xuan Zhang

Clinical immunology (Orlando, Fla.) 229:108795 PubMed34252574

2021

Complement activation and increased expression of Syk, mucin-1 and CaMK4 in kidneys of patients with COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Simin Jamaly,Maria G Tsokos,Rhea Bhargava,Olga R Brook,Jonathan L Hecht,Reza Abdi,Vaishali R Moulton,Abhigyan Satyam,George C Tsokos

Frontiers in cell and developmental biology 8:734 PubMed32850846

2020

Wnt Signaling Pathway Dysregulation in the Aging Brain: Lessons From the .

Applications

Unspecified application

Species

Unspecified reactive species

Nibaldo C Inestrosa,Cheril Tapia-Rojas,Carolina B Lindsay,Juan Manuel Zolezzi

Bioscience reports 39: PubMed31769476

2019

Mechanism of AMPK-mediated apoptosis of rat gastric smooth muscle cells under high glucose condition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Zi Zhang,Mo-Han Zhang,Xue-Sen Fang,Xiang-Shu Cui,Zheng Jin

Molecular neurobiology 56:1870-1882 PubMed29967987

2018

Wnt-7a Stimulates Dendritic Spine Morphogenesis and PSD-95 Expression Through Canonical Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Ramos-Fernández,Cheril Tapia-Rojas,Valerie T Ramírez,Nibaldo C Inestrosa

Molecular brain 11:10 PubMed29463281

2018

Soluble Aβ increases the heterogeneity in synaptic vesicle pool size among synapses by suppressing intersynaptic vesicle sharing.

Applications

Unspecified application

Species

Unspecified reactive species

Daehun Park,Sunghoe Chang

Proceedings of the National Academy of Sciences of 114:E8741-E8749 PubMed28973908

2017

Substance P induces plasticity and synaptic tagging/capture in rat hippocampal area CA2.

Applications

Unspecified application

Species

Unspecified reactive species

Ananya Dasgupta,Nimmi Baby,Kumar Krishna,Muhammad Hakim,Yuk Peng Wong,Thomas Behnisch,Tuck Wah Soong,Sreedharan Sajikumar

Proceedings of the National Academy of Sciences of 114:E619-E628 PubMed28069946

2017

Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Ming Chen,Lauren L Orefice,Shu-Ling Chiu,Tara A LeGates,Samer Hattar,Richard L Huganir,Haiqing Zhao,Baoji Xu,Rejji Kuruvilla

Nature cell biology 17:665-77 PubMed25915125

2015

The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development.

Applications

WB

Species

Unspecified reactive species

Xunlei Kang,Zhigang Lu,Changhao Cui,Mi Deng,Yuqi Fan,Baijun Dong,Xin Han,Fuchun Xie,Jeffrey W Tyner,John E Coligan,Robert H Collins,Xiangshu Xiao,M James You,Cheng Cheng Zhang

Molecular brain 3:27 PubMed20846411

2010

Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice.

Applications

IHC

Species

Unspecified reactive species

Hiroki Toyoda,Ming-Gao Zhao,Valentina Mercaldo,Tao Chen,Giannina Descalzi,Satoshi Kida,Min Zhuo
View all publications
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