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Rabbit Recombinant Monoclonal CAMKIV antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.


Images

Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (AB68218), expandable thumbnail
  • Western blot - Anti-CAMKIV antibody [EP2565AY] (AB68218), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.

Alternative names

CAMK, CAMK-GR, CAMKIV, CAMK4, Calcium/calmodulin-dependent protein kinase type IV, CaMK IV, CaM kinase-GR

Recommended products

Rabbit Recombinant Monoclonal CAMKIV antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP2565AY
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched from a hybridoma to recombinant production method on 29th August 2024.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein known as CaMKIV (Calcium/Calmodulin-dependent Protein Kinase IV) plays a role in various cellular processes. Mechanically CaMKIV is a serine/threonine protein kinase with a molecular weight of approximately 52 kDa. It depends on calcium ions and calmodulin for activation. The protein is expressed in several tissues including the brain thymus and testis. It is known for its involvement in phosphorylating substrates which then activate transcription factors.

Biological function summary

CaMKIV takes part in the regulation of gene expression and is involved in neural signaling cell cycle control and immune responses. This kinase forms part of a larger signaling complex that facilitates specific cellular events. Its activity affects processes such as long-term potentiation and T-cell maturation indicating its significance in both the nervous and immune systems.

Pathways

CaMKIV integrates into several signaling pathways including the calcium signaling pathway and CREB (cAMP response element-binding protein) pathway. Through these pathways CaMKIV activates CREB via phosphorylation linking it to gene transcription processes essential for memory formation. CaMKIV acts alongside proteins like other CaM kinases such as CaMKI and CaMKII to ensure efficient signal transduction.

Associated diseases and disorders

CaMKIV's function impacts conditions like neurodegenerative diseases and autoimmune disorders. Dysregulation of CaMKIV may relate to Alzheimer's disease where altered calcium homeostasis contributes to pathogenesis. The protein also connects to autoimmune diseases through its interaction with NFAT (nuclear factor of activated T-cells) which influences immune responses and might lead to disorders when dysregulated.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (ab68218), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (ab68218)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling CAMKIV with ab68218 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution, was co-incubated with ab68218 overnight at 4° C. Nucleus were visualized using DAPI.

  • Western blot - Anti-CAMKIV antibody [EP2565AY] (ab68218), expandable thumbnail

    Western blot - Anti-CAMKIV antibody [EP2565AY] (ab68218)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1, 5 seconds; Lane 2, 20 seconds.

    All lanes: Western blot - Anti-CAMKIV antibody [EP2565AY] (ab68218) at 1/10000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

    Lane 2: Human brain lysate at 15 µg

    Secondary

    Lane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Lane 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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