AB68218

Anti-CAMKIV antibody [EP2565AY]

Name: Anti-CAMKIV antibody [EP2565AY] (ab68218) | Abcam
Brand: Abcam Limited
SKU: AB68218
Price: 620 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal CAMKIV antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CAMK, CAMK-GR, CAMKIV, CAMK4, Calcium/calmodulin-dependent protein kinase type IV, CaMK IV, CaM kinase-GR

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (AB68218)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (AB68218)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling CAMKIV with ab68218 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab68218 overnight at 4° C. Nucleus were visualized using DAPI.

Lab

Western blot - Anti-CAMKIV antibody [EP2565AY] (AB68218)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1, 5 seconds; Lane 2, 20 seconds.

All lanes:

Western blot - Anti-CAMKIV antibody [EP2565AY] (ab68218) at 1/10000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

Human brain lysate at 15 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 2:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Carrier free

Anti-CAMKIV antibody [EP2565AY] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CAMKIV antibody [EP2565AY]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2565AY

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

This product has switched from a hybridoma to recombinant production method on 29th August 2024.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as CaMKIV (Calcium/Calmodulin-dependent Protein Kinase IV) plays a role in various cellular processes. Mechanically CaMKIV is a serine/threonine protein kinase with a molecular weight of approximately 52 kDa. It depends on calcium ions and calmodulin for activation. The protein is expressed in several tissues including the brain thymus and testis. It is known for its involvement in phosphorylating substrates which then activate transcription factors.

Biological function summary

CaMKIV takes part in the regulation of gene expression and is involved in neural signaling cell cycle control and immune responses. This kinase forms part of a larger signaling complex that facilitates specific cellular events. Its activity affects processes such as long-term potentiation and T-cell maturation indicating its significance in both the nervous and immune systems.

Pathways

CaMKIV integrates into several signaling pathways including the calcium signaling pathway and CREB (cAMP response element-binding protein) pathway. Through these pathways CaMKIV activates CREB via phosphorylation linking it to gene transcription processes essential for memory formation. CaMKIV acts alongside proteins like other CaM kinases such as CaMKI and CaMKII to ensure efficient signal transduction.

CaMKIV's function impacts conditions like neurodegenerative diseases and autoimmune disorders. Dysregulation of CaMKIV may relate to Alzheimer's disease where altered calcium homeostasis contributes to pathogenesis. The protein also connects to autoimmune diseases through its interaction with NFAT (nuclear factor of activated T-cells) which influences immune responses and might lead to disorders when dysregulated.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.

See full target information CAMK4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:276 PubMed39465429

2024

Fenebrutinib, a Bruton's tyrosine kinase inhibitor, blocks distinct human microglial signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Langlois,Simona Lange,Martin Ebeling,Will Macnair,Roland Schmucki,Cenxiao Li,Jonathan DeGeer,Tania J J Sudharshan,V Wee Yong,Yun-An Shen,Christopher Harp,Ludovic Collin,James Keaney

Nature communications 13:4255 PubMed35869084

2022

Calcium/calmodulin-dependent protein kinase IV promotes imiquimod-induced psoriatic inflammation via macrophages and keratinocytes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Yong,Yafen Yu,Bao Li,Huiyao Ge,Qi Zhen,Yiwen Mao,Yanxia Yu,Lu Cao,Ruixue Zhang,Zhuo Li,Yirui Wang,Wencheng Fan,Chang Zhang,Daiyue Wang,Sihan Luo,Yuanming Bai,Shirui Chen,Weiwei Chen,Miao Liu,Jijia Shen,Liangdan Sun

Journal of biomedical materials research. Part B, Applied biomaterials 110:1594-1603 PubMed35106922

2022

Magnesium promotes osteogenesis via increasing OPN expression and activating CaM/CaMKIV/CREB1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Hou,Yu Sun,Weichao Yang,Hongliu Wu,Luyuan Sun,Xinjie Xiu,Chaoyang Xiu,Xiaonong Zhang,Wen Zhang

Molecular neurobiology 57:1622-1635 PubMed31808063

2019

Kir6.1 Heterozygous Mice Exhibit Aberrant Amygdala-Dependent Cued Fear Memory.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Inagaki,Shigeki Moriguchi,Kohji Fukunaga

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAMKIV antibody [EP2565AY]

Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (AB68218)

Western blot - Anti-CAMKIV antibody [EP2565AY] (AB68218)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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