Rabbit Polyclonal CAMKK2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CAMKK2 aa 1-50.
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Publications
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- Computational and mathematical methods in medicine 2022:71879402022CaMKK2 Promotes the Progression of Ovarian Carcinoma through the PI3K/PDK1/Akt Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Chen,Xingxing Sun,Zhiyan Xia,Jihong Wang,Nan Guo,Yi ZhangPubMed 35309839
- Folia histochemica et cytobiologica 60:74-882022Insulin-like growth factor-1 inhibits apoptosis of rat gastric smooth muscle cells under high glucose condition via adenosine monophosphate-activated protein kinase (AMPK) pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang-Zi Zhang,Yan Sun,Mo-Han Zhang,Zheng JinPubMed 35156189
- Folia histochemica et cytobiologica 59:57-652021miR-378a-5p regulates CAMKK2/AMPK pathway to contribute to cerebral ischemia/reperfusion injury-induced neuronal apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Zhang,Peilan Zhang,Chunying DengPubMed 33651374
- Molecular cell 77:951-969.e92020AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal Damage via a Novel Galectin-Directed Ubiquitin Signal Transduction System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyue Jia et. al.PubMed 31995728
- JPEN. Journal of parenteral and enteral nutrition 44:481-4902019A Comparison of Gastric and Jejunal Feeding in Hypercatabolism Associated With Hypothalamic AMPK-Autophagy-POMC in Endotoxemic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun Cao,Xiaohua Li,Xiaodong Yang,Fengchan Xi,Tao Gao,Chungen Xing,Wenkui YuPubMed 31148205
- Autophagy 13:82-982016Store-operated calcium entry-activated autophagy protects EPC proliferation via the CAMKK2-MTOR pathway in ox-LDL exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Yang,Jie Yu,Dongdong Li,Sanjiu Yu,Jingbin Ke,Lianyou Wang,Yanwei Wang,Youzhu Qiu,Xubin Gao,Jihang Zhang,Lan HuangPubMed 27791458
- CNS neuroscience & therapeutics 22:518-242016CAMKK2, Regulated by Promoter Methylation, is a Prognostic Marker in Diffuse Gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDa-Ming Liu et. al.PubMed 27012733
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CAMKK2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q96RR4
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
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Target data
Function
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
Alternative names
CAMKKB, KIAA0787, CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, CaM-KK 2, CaM-kinase kinase 2, CaMKK 2, Calcium/calmodulin-dependent protein kinase kinase beta, CaM-KK beta, CaM-kinase kinase beta, CaMKK beta
Recommended products
Rabbit Polyclonal CAMKK2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CAMKK2 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CAMKK2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q96RR4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) also known as CAMK or CAMKK-beta is an important protein kinase. It has a mass of approximately 66 kDa and is mainly expressed in the brain but also found in other tissues such as the liver and pancreas. CAMKK2 functions as an upstream kinase activating downstream kinases including CAMK1 and CAMK4. Through these interactions CAMKK2 plays an integral role in various cellular processes such as calcium signaling.
- Biological function summary
Calcium/calmodulin-dependent protein kinase kinase 2 influences neuronal function through its regulation of calcium signaling pathways. CAMKK2 participates in the formation of a complex with calmodulin when calcium levels rise. This interaction modulates neurotransmitter release neuronal growth and synaptic plasticity. Beyond the nervous system CAMKK2 contributes to metabolic processes in the liver and insulin secretion in the pancreas thereby affecting whole-body energy homeostasis.
- Pathways
Calcium/calmodulin-dependent protein kinase kinase 2 is involved in the calcium signaling and AMP-activated protein kinase (AMPK) pathways. In these pathways CAMKK2 interacts with CAMK1 CAMK4 and AMPK influencing cellular activities like energy balance and apoptosis. The activation of AMPK by CAMKK2 plays a significant role in maintaining cellular energy levels by regulating glucose and lipid metabolism linking it to diverse physiological functions.
- Associated diseases and disorders
Dysregulation of calcium/calmodulin-dependent protein kinase kinase 2 has connections to metabolic diseases and neurodegenerative disorders such as type 2 diabetes and Alzheimer's disease. Aberrant CAMKK2 activity can alter insulin signaling and energy homeostasis contributing to the development of type 2 diabetes. Additionally its interaction with calmodulin and downstream kinases may influence tau phosphorylation an important process in Alzheimer's pathology further illustrating the importance of CAMKK2 in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CAMKK2 antibody (ab96531)
All lanes: Western blot - Anti-CAMKK2 antibody (ab96531) at 1/500 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 65 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CAMKK2 antibody (ab96531)
Immunofluorescence analysis of paraformaldehyde fixed HeLa using ab96531 at 1/200 dilution: (1) without DNA probe; (2) merged with DNA probe.
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