AB96531

Anti-CAMKK2 antibody

Name: Anti-CAMKK2 antibody (ab96531) | Abcam
Brand: Abcam Limited
SKU: AB96531
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal CAMKK2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human CAMKK2 aa 1-50.

View Alternative Names

CAMKKB, KIAA0787, CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, CaM-KK 2, CaM-kinase kinase 2, CaMKK 2, Calcium/calmodulin-dependent protein kinase kinase beta, CaM-KK beta, CaM-kinase kinase beta, CaMKK beta

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKK2 antibody (AB96531)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKK2 antibody (AB96531)

Immunohistochemical analysis of paraffin-embedded human endometrial cancer, using ab96531 at 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-CAMKK2 antibody (AB96531)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-CAMKK2 antibody (ab96531) at 1/1000 dilution

Lane 1:

IMR-32 whole cell lysates at 30 µg

Lane 2:

SK-N-SH whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CAMKK2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q96RR4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) also known as CAMK or CAMKK-beta is an important protein kinase. It has a mass of approximately 66 kDa and is mainly expressed in the brain but also found in other tissues such as the liver and pancreas. CAMKK2 functions as an upstream kinase activating downstream kinases including CAMK1 and CAMK4. Through these interactions CAMKK2 plays an integral role in various cellular processes such as calcium signaling.

Biological function summary

Calcium/calmodulin-dependent protein kinase kinase 2 influences neuronal function through its regulation of calcium signaling pathways. CAMKK2 participates in the formation of a complex with calmodulin when calcium levels rise. This interaction modulates neurotransmitter release neuronal growth and synaptic plasticity. Beyond the nervous system CAMKK2 contributes to metabolic processes in the liver and insulin secretion in the pancreas thereby affecting whole-body energy homeostasis.

Pathways

Calcium/calmodulin-dependent protein kinase kinase 2 is involved in the calcium signaling and AMP-activated protein kinase (AMPK) pathways. In these pathways CAMKK2 interacts with CAMK1 CAMK4 and AMPK influencing cellular activities like energy balance and apoptosis. The activation of AMPK by CAMKK2 plays a significant role in maintaining cellular energy levels by regulating glucose and lipid metabolism linking it to diverse physiological functions.

Dysregulation of calcium/calmodulin-dependent protein kinase kinase 2 has connections to metabolic diseases and neurodegenerative disorders such as type 2 diabetes and Alzheimer's disease. Aberrant CAMKK2 activity can alter insulin signaling and energy homeostasis contributing to the development of type 2 diabetes. Additionally its interaction with calmodulin and downstream kinases may influence tau phosphorylation an important process in Alzheimer's pathology further illustrating the importance of CAMKK2 in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.

See full target information CAMKK2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 12:1557509 PubMed40171409

2025

AMPK and CAMKK activation participate in early events of -triggered NET formation in bovine polymorphonuclear neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Iván Conejeros,Zahady D Velásquez,Gabriel Espinosa,Lisbeth Rojas-Baron,Magdalena Grabbe,Carlos Hermosilla,Anja Taubert

International journal of molecular sciences 25: PubMed39126009

2024

The CAMKK/AMPK Pathway Contributes to -Induced NETosis in Bovine Polymorphonuclear Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Iván Conejeros,Zahady D Velásquez,Lisbeth Rojas-Barón,Gabriel Espinosa,Carlos Hermosilla,Anja Taubert

Computational and mathematical methods in medicine 2022:7187940 PubMed35309839

2022

CaMKK2 Promotes the Progression of Ovarian Carcinoma through the PI3K/PDK1/Akt Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Chen,Xingxing Sun,Zhiyan Xia,Jihong Wang,Nan Guo,Yi Zhang

Folia histochemica et cytobiologica 60:74-88 PubMed35156189

2022

Insulin-like growth factor-1 inhibits apoptosis of rat gastric smooth muscle cells under high glucose condition via adenosine monophosphate-activated protein kinase (AMPK) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Zi Zhang,Yan Sun,Mo-Han Zhang,Zheng Jin

Folia histochemica et cytobiologica 59:57-65 PubMed33651374

2021

miR-378a-5p regulates CAMKK2/AMPK pathway to contribute to cerebral ischemia/reperfusion injury-induced neuronal apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhang,Peilan Zhang,Chunying Deng

Molecular cell 77:951-969.e9 PubMed31995728

2020

AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal Damage via a Novel Galectin-Directed Ubiquitin Signal Transduction System.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyue Jia,Bhawana Bissa,Lukas Brecht,Lee Allers,Seong Won Choi,Yuexi Gu,Mark Zbinden,Mark R Burge,Graham Timmins,Kenneth Hallows,Christian Behrends,Vojo Deretic

JPEN. Journal of parenteral and enteral nutrition 44:481-490 PubMed31148205

2019

A Comparison of Gastric and Jejunal Feeding in Hypercatabolism Associated With Hypothalamic AMPK-Autophagy-POMC in Endotoxemic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Cao,Xiaohua Li,Xiaodong Yang,Fengchan Xi,Tao Gao,Chungen Xing,Wenkui Yu

Autophagy 13:82-98 PubMed27791458

2016

Store-operated calcium entry-activated autophagy protects EPC proliferation via the CAMKK2-MTOR pathway in ox-LDL exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yang,Jie Yu,Dongdong Li,Sanjiu Yu,Jingbin Ke,Lianyou Wang,Yanwei Wang,Youzhu Qiu,Xubin Gao,Jihang Zhang,Lan Huang

CNS neuroscience & therapeutics 22:518-24 PubMed27012733

2016

CAMKK2, Regulated by Promoter Methylation, is a Prognostic Marker in Diffuse Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Da-Ming Liu,Hong-Jun Wang,Bo Han,Xiang-Qi Meng,Ming-Hui Chen,Dong-Bo Yang,Ying Sun,Yong-Li Li,Chuan-Lu Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAMKK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKK2 antibody (AB96531)

Western blot - Anti-CAMKK2 antibody (AB96531)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise